Studies on the Effect of Gamma, Laser Irradiation and Progesterone Treatments on Gerbera Leaves
European Journal of Biophysics
Volume 3, Issue 6, December 2015, Pages: 43-50
Received: Nov. 3, 2015; Accepted: Nov. 17, 2015; Published: Dec. 10, 2015
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Sami Ali Metwally, Ornamental Plants and Woody Trees Department, National Research Centre, Dokki, Egypt
Abd-El Rhaman Elariean Awad, Horticulture Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt
Bedour Helmy Abou-Leila, Water Relations and Field Irrigation Department, National Research Centre, Dokki, Egypt
Tarek Abd Allah El Tayeb, Photochemistry and Photobiology, National Institute of Laser Enhanced Sciences, Cairo University, Dokki, Egypt
Ibrahem El Abasery Habba, Ornamental Plants and Woody Trees Department, National Research Centre, Dokki, Egypt
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The present study, we aimed to evaluate the effect of progesterone (prog.) concentrations (10, 20 and 30 ppm), gamma rays (γ) doses (100,150 and 250 rad), helium neon laser rays (He-Ne) exposure time (1.0, 5.5 and 11.5 min.) and argon laser rays (Ar) exposure time (1.0, 7.5 and 15.5 min.) and some interaction treatments between progesterone and irradiation treatments on biochemical constituents i.e. growth regulators and some enzymes activity in gerbera leaves. For this reason, at the greenhouse of the National Research Centre, Dokki, Giza, Egypt. Pot experiments were carried out two successive seasons of 2006 and 2007, and growth regulators (IAA, GA3, Cytok. and ABA), some enzymes activity in gerbera leaves (POD, CAT, Alk. phosph and Acid. phosph) and protein content were determined. The results showed that, low conc. of progesterone hormone, low exposure time of argon and He-Ne laser has a great effect on increasing POD activities and Cytokine content. Gamma rays at 150 rad increased ABA, POD, CAT and Alkaline and acid phosphatase, while 250 rad increased IAA and GA-like substances. The least value of alkaline phosphatase activity was recorded by progesterone treatments.
Gerbera, Chemical Constituents, Gamma Radiation, Laser Rays, Progesterone, Enzymes Activity, Growth Hormones
To cite this article
Sami Ali Metwally, Abd-El Rhaman Elariean Awad, Bedour Helmy Abou-Leila, Tarek Abd Allah El Tayeb, Ibrahem El Abasery Habba, Studies on the Effect of Gamma, Laser Irradiation and Progesterone Treatments on Gerbera Leaves, European Journal of Biophysics. Vol. 3, No. 6, 2015, pp. 43-50. doi: 10.11648/j.ejb.20150306.11
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Lebouef, M.; Cave, A. and Goutarel, R. (1964): Alkaloides steroidiques presence de la progesterone dans les feuilles de le Holarrhena floribunda (G. Don) Dur. et Schinz. Compt. Rend. Acad. Sci. Paris 259: 3401-3403. (c.f. Phytochemistry 68 (2007).
Gawienowski, A. M. and Gibbs, C. C. (1968): Identification of cholesterol and progesterone in apple seeds. Steroids, 12: 440-545.
Lino, M.; Nomura, T.; Tamaki, Y.; Yamada, Y.; Yoneyama, K.; Takeuchi, Y.; Mori, M.; Asami, T.; Nakano, T. and Yokota, T.( 2007): Progesterone: its occurrence in plants and involvement in plant growth. Phytochemistry, 68 (12): 1664-1673.
Janeczko, A. and Filek, W. (2002): Stimulation of generative development in partly vernalized winter wheat by animal sex hormones. Acta Physiol. Plant, 24: 291-295.
Janeczko, A.W.; FilekBiesaga K. J.; Marcinska, I. and Janeczko, Z. (2003): The influence of animal sex hormones on the induction of flowering in Arabidopsis thaliana comparison with the effect of 24 epibrassinolide. Plant Cell, Tissue and Organ Culture 72: 147-151.
Mucip, G.; Hulya, T. and Serkan, E.( 2013): Exogenous progesterone application protects chickpea seedlings against chilling-induced oxidative stress. Acta Physiol Plant, 35: 241–251.
Kim,J.H.; Kim,M.H.; BeakChung, B.Y.; Wi, S.G. and Kim, J.S.(2004):Alterations in the photosynsetic pigments and antioxidant machineries of red peper (Capsicum annuum L.) seedlings from gamma irradiated seeds. J.plant Biol.47: 314-321.
Kovacs and Kereszies (2002): Effect of gamma and UV-B/C radiation on plant cell. Micron, 33: 199-210.
Wi, S. G; Chung, B. Y.; Kim, J. H.; Beak, M. H.; Yang, D. H.; Lee, J. W and Kim, J. S. (2005): Ultrastuctural changes of cell organelles in Arabidopsis stem after gamma irradiation, J. Biol., 48 (2): 195-200.
Berns, M.W.; Nelson, T. S. and Wright, W. H. (1992): Laser physics and laser Tissue Interactions. Laser in Plastic Surgery and Dermatology (B.M. Achouer, V. Mender and M. W. Bernseds). 2nd ed. 1-10, New York.
Pick, R. H. (1993): Using laser in clinical dental practice JADA, 124: 37-47. (c.f. M.Sc.Thesis, National Institute of laser. Cairo Univ.)
Maamoun, M.K.M.; El-Mahrouk, M.E.; Dewir, Y.H. and Omran, S.A. (2014): Effect of radiation and chemical mutagens on seeds germination of black cumin (Nigella sativa L). Journal of Agricultural Technology, 10(5): 1183- 1199.
Glenn, T.L.; Kuo, C.C.; Durly, R.C. and pharis, R.P. (1972): Use of insoluble polyvinyl pyrrolidone for purification of plant extracts and chromatography of plant hormones. Phytochemistry, 11: 345-351.
Van Staden, J. (1973): Changes in endogenous cytokinine levels during abscission and senescence of streptocarpus leaves. J. of Exp. Bot. 21: 667-673.
Vitoria, A. P.; Lea, P. J. and Azevado, R. A. (2001): Antioxidant enzymes responses to cadmium in radish tissues. Phytochemistry, 57: 701-710.
Chance, B. and Moehly, A.C. (1955): Assay of Catalase and Peroxidase. Methods in Enzymology, 2: 762-755.
Amako, A.; Chen, K. and Asada, K. (1994): Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosilic isoenzymes of ascorbate peroxidase in plants. Plant Cell Physiology, 35: 497-504.
Layne, E. (1957): Spectrophotometric and Turbidimetric Methods for Measuring Proteins. Methods in Enzymology. 10: 447-455.
Sebanek, J.; Kralik. J.; Hudeova, M.; Klicova; Slaby, K.; Psota, V.; Vitkova, H.; Polisenka, M.; Kudova, D.; Sterba, S. and Vancura, J. (1989): Growth and hormonal effects of laser on germination and rhizogenesis in plants. Providovedna Prace Ustavu Ceskoslovenske Akademie Ved V Brne., 23(9): 49-69.
Batov, I. and Kitin, P. (1993): Results of laser treatment of ripe cuttings of Robinia Pseudoacacia. Nauka Za Gorata, 30(3): 10-21.
Awad, A. R.E and El Bahr, K. M. (1986): Growth regulators and gamma irradiation treatments affecting the sprouting and endogenous hormones in gladiolus. Acta Horticulture, No.177, vol.II, 612.
Gaber, A.M; Mostafa, H.A.M. and Ramadan, A.A. (2000): Effect of gamma irradiation of faba bean (Viciafaba) plant on its chemical composition, favism causative agents and hormonal levels. Egyptian Journal of Physiological Sciences, 24 (1): 1-16.
Guo, J.; Liu, Y.; Tang, J. and Liu, C. (1983): Mutations in roses induced by gamma rays. Journal of Nanjing Technological College of Forest Products, No.2: 68-74.
Ebtihal, M. AE. (2008): Physiological effects of some phytoregulators on growth, productivity and yield of wheat plant cultivated in new reclaimed soil. Ph.D. Thesis. Collage of Women for Arts, Science and Education. Ain Shams Univ.
Frylink, L. Dubery, I. A and Schabor, J.C. T. (1987): Biochemical changes involved in stress response and ripening behavior of gamma irradiated mango fruit. Photochemistry, 26 (3): 681-686.
Kamel, H.A. (2009): Effects of seed irradiation on c14 fixation and antioxidant activity of vitamin C and total phenols of canola leaves. Isotope and RAD. RES. 404 (suppl.1), 1353-1361.
Dae, Y. K.; Min, J. H.; Cheong, S. P. and Yong, W. S. (2015): The effects of chronic radiation of gamma ray on protein expression and oxidative stress in Brachypodium distachyon. International Journal of Radiation Biology, 91(5): 407-419.
Jain, J. and Khanna, V.K. (1986): Effect of gamma radiation on peroxidase activity in developing grains of triticale. Indian Journal of Plant Physiology, 29 (4): 308-311.
Nigmanov, V.A.; Pak, V.M. and Kurnetsova, O.N. (1989): The radio protective effect of magnesium chloride on radix enzyme activity and chlorophyll contents in cotton plants. Uzbekiston Biolagija Zurnali, 2: 3-5.
Liu Qinglin; Wu DiXin; Tian YanTing; Liu QL; Wu DX; Tian, YT (2000): Preliminary report on induced mutation of Iris somaclonals. Acta Horticulturae, No.404: 91-94.
Ahmed, S. O. I. (2004): Biochemical changes of potatoes during growth and storage as affected by gamma radiation Ph.D. Thesis, Department of Biochemistry. Faculty of Agric. Cairo, Univ.
Moussa, H.R. (2008): Gamma irradiation affects on antioxidant enzymes and G6PDH activities in Vicia faba plants. Journal of New Seeds, 9(1): 89-99.
Zong BoQiu; Xiaoliu; Xiang Jun; Tian; and Ming Yue. (2008): Effect of Co2 laser pretreatment on drought stress resistance in wheat. Journal of Photochemistry and Photobiology, 90: 17-25.
Grisyuk, S. N. (1985): Effect of laser light on rooting of rose cuttings. Introduktsiya AKK limatizatsiya Rastenii, No.3: 51-52.
Beltagi, M.S.; Ismail, M.A and Mohamed, F.H.(2006):Induced salt tolerance in common bean (Phaseolus vulgaris L.) by gamma irradiation. Pakistan Journal of Biological Sciences, 9(6): 1143-1148.
Wessam, M. S. E. A. (2005): Effect of laser on the growth and on the active constituents of sage plant. M.Sc. Thesis, National Institute of laser. Cairo Univ.
Kalinich, J.F.; Mandava, N.B. and Todhunter, J.A. (1985): Relationship of nucleic acid metabolism to brassinolide induced responses in beans. J. Plant Physiol, 120: 207-14.
Blushan, N. (1988): Plant growth promoting brassinosteroids. Plant Physiol., 39: 23-52.
Braun, P. and Wild, A.(1984a):The influence of brassinosteroid on growth promoting steroidal lactone on development and carbon dioxide fixation capacity of intact wheat and mustard seedlings. In Advances in Photosynthesis Research, Proc. 6th Cong. photosynthesis, cd. Sybesna, 3: 461-464.
Yasutomo Takeuchi; Yumi Omigawa; Masaru; Ogasawara; Koichi Yoneyama; Makoto Konnai and Douglas Worsham, A. (1995): Effects of brassinosteroids on conditioning and germination of clover broomrape Orobanche minor seeds. Plant Growth Regulation, 16: 153-160.
Krizek, D.T. and Mandova, N.B.(1983):Influence of spectral quality on the growth response of intact bean plants to brassinosteroid, a growth promoting steroidal lactone,7 stem elongation and morphyegenesis. Physical Plant, 57: 317-323.
Mandava, N.B. (1988): Plant growth promoting brassinosteroids. Ann. Rev. Plant Mol, 39: 23-52.
Cohen, J.D. and Meudt, W.J. (1983): Investigations on the mechanism of brassinosteroid response. I. Indol 3 acetic acid metabolism and transport. Plant Physiol., 72: 691-694.
Ray, S. and Choudhuri, M. A. (1981): Effect of growth regulators on grain and yield of rice Oryza Sativa L. Ann. Bol. (London) 47, 755.
Innes, P.; Black Well, R.D. and Quarrie, S.A. (1984): Some effects of genetic variation in drought induced abscisic acid accumulation on the yield and water use of spring wheat. J. Agric. Sci. 102, 341.
Dewdeny, S.J. and Mcwha, J.A. (1979): Abscisic acid and the movement of photosynthetic assimulates towards developing wheat (Triticum aestivium) grains. Z. Phamzen, Physiol. 92: 183.
Chanda, E. and Singh, H. (1997): Changes in peroxidase and IAA oxidase activities during wheat grain development. Plant Physiol. Biochem, 35: 245-250.
Espelie, K. E.; Francesci, V.R. and Kolattukudy, P.E. (1986): Immuno cytochemical localization and time course of appearance of an anionic peroxidase associated with suberization in wound healing potato tuber tissue. Plant Physiol, 81: 487-492.
Lagrimini, L.M.; Burkhat, W. and Moyer, M. (1987): Molecular cloning of complementary DNA encoding the lignin forming peroxidase from tobacco molecular analyses and tissue specific expression, Proc. Natl. Acad. Sci. USA 84: 7542-7546.
Kamiya, Y.L. Jose and Martinez, G. (1999): Regulation of gibberellin biosynthesis by light. Current Openion in Plant Biology, 2: 398-403.
Van and Overbeek, J. (1966): Plant hormones and regulators science. U.S.A. 152: 721-731. W.H. Freeman and Company, San Francisco. (c.f. M.Sc. Thesis, National Institute of laser. Cairo Univ.)
Kuraishi, S. and Muir, K.M. (1963): Mode of action of growth retarding. Plant Physiol., 38: 19-24.
Katsumi, M. and Sano, H. (1968): Changes in indole acetic oxidase levels in leaves of Begonia evansiana as they ages. Plant Cell Physiol, 15: 869.
Macleod, A. M. and Millar, A. S. (1962): Effect of gibberellic acid on basley endosperm. J. Inst. Brewing 66: 322-332. W.H. Freeman and Company, San Francisco. U.S.A. (c.f. M.Sc. Thesis, National Institute of laser. Cairo Univ.)
Arun Dev Sharma, Neha, S. and Jagjeet. K. K. (2005): Short term water legging induced changes in phosphatase activities in shoots and roots of sorghum seedlings role of phosphatases during water logging in relation to phosphatases. Plant Physiology, 3(1-2): 71-79.
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