UV Absorbance of Aqueous DNA
European Journal of Biophysics
Volume 3, Issue 3, June 2015, Pages: 19-22
Received: May 7, 2015; Accepted: May 13, 2015; Published: Jun. 1, 2015
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Author
Pivovarenko Yuri Vadimovich, Research and Training Center ‘Physical and Chemical Materials Science’ under Kyiv Taras Shevchenko University and NAS of Ukraine, Kiev, Ukraine
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Abstract
The UV absorption spectra of water with different electric charge (potential) were obtained. It was shown that UV absorption spectra of water with negative electric charge (potential) have the sharp peaks with maximum in the range 190 – 200 nm and UV absorption spectra of water with positive electric charge (potential) have the wide peaks with maximum in the range 200 – 220 nm. It was asked to explain this absorption. It was also established that UV absorption spectra of water solutions of surface inactive substances have sharp peaks with a maximum in the range 190 – 200 nm and UV absorption spectra of solutions of surface active substances have the wide peaks with a maximum in the range 200 – 220 nm. The UV absorption spectra of DNA solutions, which were prepared on the water with different electric charge (potential), were obtained. It was shown that these spectra are dependent on the electric charge (potential) of water used. It was proposed that UV absorption spectra of aqueous DNA reflect mostly the spectral properties of charged water or charged cuvette.
Keywords
UV Absorption Spectra, Electric Charge, Electric Potential, DNA
To cite this article
Pivovarenko Yuri Vadimovich, UV Absorbance of Aqueous DNA, European Journal of Biophysics. Vol. 3, No. 3, 2015, pp. 19-22. doi: 10.11648/j.ejb.20150303.11
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