International Journal of Genetics and Genomics

| Peer-Reviewed |

Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan

Received: 03 April 2019    Accepted: 17 May 2019    Published: 23 September 2019
Views:       Downloads:

Share This Article

Abstract

Pakistan is blessed with rich sheep genetic resources and being a source of milk, meat and wool sheep breeds are reared across the country. Despite its great economic importance very limited work has been done in Pakistan on sheep genetic exploration. Leptin hormone plays important role in milk yield, body weight, energy balance, feed intake, immune function and fertility performance in animals. The current study was carried out to investigate genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP analysis revealed that all the three studied animals breeds were polymorphic for i>LEP gene (having all the three genotypes AA, AB and BB) with frequency of 60%, 33% and 7% in Nili-ravi buffalo, 55%, 41% and 4% in Achai cattle and 52%, 45% and 3% in Sahiwal cattle breed. The genotype BB is highly desirable in cattle and buffalo breeds but its frequency is very low in our indigenous population. Studying genetic polymorphism of milk protein is important due to its application in dairy industry and these in formations could further be utilized for future breeding programs particularly for the enrichment of the rare genotype.

DOI 10.11648/j.ijgg.20190703.17
Published in International Journal of Genetics and Genomics (Volume 7, Issue 3, September 2019)
Page(s) 75-79
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Leptin, Sahiwal, Achai, Nili-Ravi, Polymorphism, PCR-RFLP

References
[1] Shah, S. K. 1991. Buffaloes of Pakistan. Pakistan Agriculture Council, Islamabad, pp: 64-82.
[2] Ghafoor A. Muhammad N. R. Aamer B. Z. Nasir A. Muhammad Y. Abdullah S. Rafaqat I. and Muhammad S. 2014. Κ-CN gene polymorphism in Nili-ravi buffalo, Achai and Sahiwal cattle of Pakistan. Inter. J. Dairy tech, 67; 1-6.
[3] Kemenes, P. A. L. C. A. Regitano, A. J. M. Rosa, I. U. Packer, A. G. R alook, L. A. Figueirede, N. A. Silva, M. Antonia, L. Etchegarary and L. L. Coutinho, 1999. Kappa-casein, beta-lactoglobulin and growth hormonr allele frequencies and genetic distances in Nelore, Gry,, Guzera, Caracu, Charolais, Canchin and Santa Gertrudiscattle. Gene and Mol Biol. 22 (4); 539-541.
[4] Fernando Medrano J and Aquilar-Cordova E. 1990. Genotyping of bovine kappa-casein loci following DNA sequence amplification. Biotechnology 8: 144-146.
[5] Shah, A. Abdul A. Abdul, G. Ammir. B. Z. Muhmmad, Y. Rafaqat, I. Nasir. A. and Muhmmad, S. 2018. Molecular Analysis of CallipygeGene Mutation (C.267A>G) in Kajli, Lohi and Thalli Sheep Breeds of Pakistan. Animal Genetics: 54: 848-852.
[6] Block, S. S. W. R. Butler, R. A. Ehrhardt, A. W. Bell, M. E. Van Amburgh, and Y. R. Boisclair. 2001. Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J. Endcrinol. 171: 339–348.
[7] Liefers, C. S. Pas, W. T. Veerkamp, R. F. &Lendet, T. V. 2002. Associations between Leptin Gene Polymorphisms and Production, Live Weight, Energy Balance, Feed Intake, and Fertility in Holstein Heifers. Journal of Dairy Sciences 85: 1633-1638.
[8] Madeja, Z. Adamowicz, T. Chmurynska, A. Jankowski, T. Melonek, J. Switonski, M. &Strabel, T. 2004. Effect of Leptin gene polymorphisms on breeding value for milk production traits. Journal of Dairy Science 87: 3925-3927.
[9] Stone, R. T. Kappes, M. S. & Beattie, W. C. 1996. The bovine homolog of the obese gene maps to chromosome 4. Mammalian Genome7: 399-400.
[10] Green E. D, M. Maffei, V. V Braden, RPorenca, U De Silva, Y Zhang, SC Chua, Rleibel, J Weissanbach and JM Friedman, 1995. The human obsess (OB) gene: RNA expression pattern and mapping on the physical, cytogenic and genetic maps of chromosome 7. Genome Res, 5: 5-12.
[11] Buchanan, F. C. Kessel, A. G. Waldner, C. Christensen, D. A. Laarveld, B. & Schmutz, S. M. 2003. Hot Topic: An Association between a Leptin Single Nucleotide Polymorphism and Milk and Protein Yield. Journal of Dairy Science 86: 3164–3166.
[12] Javanmard, A. Asadzadeh, N. Banabazi, M. H. & Tavakolian, J. 2005. The allele and genotype frequencies of bovine pituitary specific transcription factor and leptin genes in Iranian cattle and buffalo populations using PCR-RFLP. Iranian Journal of Biotechnology 3: 104-108.
[13] Konfortov, B. A. Licence, V. E. & Miller, J. R. 1999. Re-sequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon. Mammalian Genome 10: 1142-1145.
[14] Lagonigro, R. Wiener, P. Pilla, F. Woolliams, A. J. & Williams, L. J. 2003. A new mutation in the coding region of the bovine leptin gene associated with feed intake. Animal Genetics 34: 371-374.
[15] Liefers, S. C. Veerkamp, R. F. Tepas, M. F. W. Delavaud, C. Chilliard, Y. Platje, M. & Van, D. L. T. 2005. Leptin promoter mutation affects leptin levels and performance traits in dairy cows. Animal Genetics 36: 111-118.
[16] Sadeghi, M. Babak, M. M. S. Rahimi, G. & Jawaremi, A. N. 2008. Effect of leptin gene polymorphism on the breeding value of milk production traits in Iranian Holstein. Animal 2: 999-1002.
[17] Naqvi, N. A. 2007. Application of Molecular Genetic Technologies in Livestock Production: Potentials for Developing Countries. Advances in Biological Research 1: 72-84.
[18] Nassiry, R. M. Shahroudi, F. E. Mousavi, A. H. & Balal. 2008. The diversity of leptin gene in Iranian native, Holstein and Brown Swiss cattle. African Journal of Biotechnology 7: 2685-2687.
[19] Sambrook J, Fritsch EF, Maniatis T (ed.) (1989) Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Lab. NY.
[20] Rosner B (ed.) (2005) Fundamentals of Biostatistics, 6th edn. Duxbury Press.
[21] Kaygisiz, A. Bengi, C. &Cilek, S. 2011. Investigation of leptin gene polymorphisms in east Anatolian red Anatolian and black cattle and determination of genetic distance from brown Swiss cattle. Journal of Animal and Plant Sciences 21: 121-125.
[22] Kulig, H. 2005. Association between leptin combined genotypes and milk performance traits of Polish Black-and-White cows. Arch. TierzDummerstorf48: 547-554.
[23] Kong, H. S. Oh, J. D. Lee, S. G. Hong, Y. S. Song, W. I. Lee, J. Kim, H. C. &Yoo, B. H. 2006. Association of Polymorphisms in the Bovine Leptin Gene with Ultrasound Measurements for Improving in Korean Cattle. Asian-Australasian Journal of Animal Sciences 19: 1691-1695.
[24] Yazdani, H. H. Rahmani, R. Edris, M. A. &Dirandeh, E. 2010. Association between A59V polymorphism in exon 3 of leptin gene and reproduction traits in cows of Iranian Holstein. African Journal of Biotechnology9: 5997-6000.
Author Information
  • National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan

  • Key State Laboratory of Cellular Stress Biology, Schoolof Life Sciences, Xiamen University, Xiamen, China; Centre for Biotechnology and Microbiology, University of Swat, Swat, Pakistan

  • National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan

  • Centre for Biotechnology and Microbiology, University of Swat, Swat, Pakistan

  • National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan

  • Department of Biotechnology Shaheed Benazir Bhutto University Sheringal Dir Upper, Khyber Pakhtunkhwa, Pakistan

  • Key State Laboratory of Cellular Stress Biology, Schoolof Life Sciences, Xiamen University, Xiamen, China

  • Key State Laboratory of Cellular Stress Biology, Schoolof Life Sciences, Xiamen University, Xiamen, China

  • National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan

  • Department of Biotechnology Shaheed Benazir Bhutto University Sheringal Dir Upper, Khyber Pakhtunkhwa, Pakistan

  • Department of Biotechnology Shaheed Benazir Bhutto University Sheringal Dir Upper, Khyber Pakhtunkhwa, Pakistan

Cite This Article
  • APA Style

    Nasir Abbas, Muhammad Suleman, Aamer Bin Zahur, Abdul Ghafoor, Farooq Rashid, et al. (2019). Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. International Journal of Genetics and Genomics, 7(3), 75-79. https://doi.org/10.11648/j.ijgg.20190703.17

    Copy | Download

    ACS Style

    Nasir Abbas; Muhammad Suleman; Aamer Bin Zahur; Abdul Ghafoor; Farooq Rashid, et al. Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. Int. J. Genet. Genomics 2019, 7(3), 75-79. doi: 10.11648/j.ijgg.20190703.17

    Copy | Download

    AMA Style

    Nasir Abbas, Muhammad Suleman, Aamer Bin Zahur, Abdul Ghafoor, Farooq Rashid, et al. Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. Int J Genet Genomics. 2019;7(3):75-79. doi: 10.11648/j.ijgg.20190703.17

    Copy | Download

  • @article{10.11648/j.ijgg.20190703.17,
      author = {Nasir Abbas and Muhammad Suleman and Aamer Bin Zahur and Abdul Ghafoor and Farooq Rashid and Amin Ullah Jan and Fazal Akbar and Shahid Ali and Abdul Aziz and Ziaul Islam and Abdullah Shah},
      title = {Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan},
      journal = {International Journal of Genetics and Genomics},
      volume = {7},
      number = {3},
      pages = {75-79},
      doi = {10.11648/j.ijgg.20190703.17},
      url = {https://doi.org/10.11648/j.ijgg.20190703.17},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijgg.20190703.17},
      abstract = {Pakistan is blessed with rich sheep genetic resources and being a source of milk, meat and wool sheep breeds are reared across the country. Despite its great economic importance very limited work has been done in Pakistan on sheep genetic exploration. Leptin hormone plays important role in milk yield, body weight, energy balance, feed intake, immune function and fertility performance in animals. The current study was carried out to investigate genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP analysis revealed that all the three studied animals breeds were polymorphic for i>LEP gene (having all the three genotypes AA, AB and BB) with frequency of 60%, 33% and 7% in Nili-ravi buffalo, 55%, 41% and 4% in Achai cattle and 52%, 45% and 3% in Sahiwal cattle breed. The genotype BB is highly desirable in cattle and buffalo breeds but its frequency is very low in our indigenous population. Studying genetic polymorphism of milk protein is important due to its application in dairy industry and these in formations could further be utilized for future breeding programs particularly for the enrichment of the rare genotype.},
     year = {2019}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan
    AU  - Nasir Abbas
    AU  - Muhammad Suleman
    AU  - Aamer Bin Zahur
    AU  - Abdul Ghafoor
    AU  - Farooq Rashid
    AU  - Amin Ullah Jan
    AU  - Fazal Akbar
    AU  - Shahid Ali
    AU  - Abdul Aziz
    AU  - Ziaul Islam
    AU  - Abdullah Shah
    Y1  - 2019/09/23
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijgg.20190703.17
    DO  - 10.11648/j.ijgg.20190703.17
    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
    SP  - 75
    EP  - 79
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20190703.17
    AB  - Pakistan is blessed with rich sheep genetic resources and being a source of milk, meat and wool sheep breeds are reared across the country. Despite its great economic importance very limited work has been done in Pakistan on sheep genetic exploration. Leptin hormone plays important role in milk yield, body weight, energy balance, feed intake, immune function and fertility performance in animals. The current study was carried out to investigate genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP analysis revealed that all the three studied animals breeds were polymorphic for i>LEP gene (having all the three genotypes AA, AB and BB) with frequency of 60%, 33% and 7% in Nili-ravi buffalo, 55%, 41% and 4% in Achai cattle and 52%, 45% and 3% in Sahiwal cattle breed. The genotype BB is highly desirable in cattle and buffalo breeds but its frequency is very low in our indigenous population. Studying genetic polymorphism of milk protein is important due to its application in dairy industry and these in formations could further be utilized for future breeding programs particularly for the enrichment of the rare genotype.
    VL  - 7
    IS  - 3
    ER  - 

    Copy | Download

  • Sections