3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope

Vladimir Boris Karpov

doi:doi:10.11648/j.ijgg.20180601.12

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3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope

Vladimir Boris Karpov

Published in International Journal of Genetics and Genomics (Volume 6, Issue 1)

Received: 6 December 2016 Accepted: 17 August 2017 Published: 26 March 2018

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Abstract

A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.

Published in	International Journal of Genetics and Genomics (Volume 6, Issue 1)
DOI	10.11648/j.ijgg.20180601.12
Page(s)	8-10
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Speckle-Noise, Fourier Holography, Mach – Zehnder Scheme, CCD Detector, Digital Image Reconstruction

References

[1]	M. Born, and E. Wojf, Principles of Optics, Oxford: Pergamon, 1964.
[2]	J. B. De Vellis, and G. O. Reynolds, Theory and Application of Holography, Mass: Addison - Wesley, Reading, 1967.
[3]	D. Gabor, “A new microscopy principle,” Nature, vol. 161, 1948, pp. 777-778.
[4]	V. B. Karpov, “Problems of bad contrast in conventional microscopy solution and speskle elimination with a laser Fourier holographic microscope.” (in print)
[5]	Biological Science, Ed. By R. Soper B. Sc., F. I. Biol, Cambridge: CAMBRIGE, 1984.
[6]	B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts and J. D. Watson, Molecular Biology of the Cell, New York: Garland, 1989.
[7]	Picture Processing and Digital Filtering, ed. by T. S. Huang Topics in Applied Physics, Berlin: Springer, 1975.
[8]	Holography, ed. by J. W. Goodman, New York: Proc. IEEE, vol. 59, No. 9, September 1971.
[9]	V. B. Karpov, “Ultrahigh contrast imaging with a new laser Fourier holographic microscope of visible range,” In Coherence Domain Methods in Biomedical Optics, ed. by V. Tuchin, Proc. SPIE, vol. 2732, 1996, pp. 168-187.
[10]	V. B. Karpov, “Study of biological objects with a new laser holographic microscope,” OSA Annual Meeting, October 3-8, 1993, Toronto, Canada, Technical Digest, WS11 and ThDD59, 1993.

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Vladimir Boris Karpov. (2018). 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope. International Journal of Genetics and Genomics, 6(1), 8-10. https://doi.org/10.11648/j.ijgg.20180601.12

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ACS Style

Vladimir Boris Karpov. 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope. Int. J. Genet. Genomics 2018, 6(1), 8-10. doi: 10.11648/j.ijgg.20180601.12

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AMA Style

Vladimir Boris Karpov. 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope. Int J Genet Genomics. 2018;6(1):8-10. doi: 10.11648/j.ijgg.20180601.12

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@article{10.11648/j.ijgg.20180601.12,
  author = {Vladimir Boris Karpov},
  title = {3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope},
  journal = {International Journal of Genetics and Genomics},
  volume = {6},
  number = {1},
  pages = {8-10},
  doi = {10.11648/j.ijgg.20180601.12},
  url = {https://doi.org/10.11648/j.ijgg.20180601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20180601.12},
  abstract = {A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.},
 year = {2018}
}

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TY  - JOUR
T1  - 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope
AU  - Vladimir Boris Karpov
Y1  - 2018/03/26
PY  - 2018
N1  - https://doi.org/10.11648/j.ijgg.20180601.12
DO  - 10.11648/j.ijgg.20180601.12
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 8
EP  - 10
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20180601.12
AB  - A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.
VL  - 6
IS  - 1
ER  -

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Author Information

Vladimir Boris Karpov

Coherent and Nonlinear Optics Department, A. M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow, Russia

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APA Style

Vladimir Boris Karpov. (2018). 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope. International Journal of Genetics and Genomics, 6(1), 8-10. https://doi.org/10.11648/j.ijgg.20180601.12

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ACS Style

Vladimir Boris Karpov. 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope. Int. J. Genet. Genomics 2018, 6(1), 8-10. doi: 10.11648/j.ijgg.20180601.12

Copy | Download

AMA Style

Vladimir Boris Karpov. 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope. Int J Genet Genomics. 2018;6(1):8-10. doi: 10.11648/j.ijgg.20180601.12

Copy | Download

@article{10.11648/j.ijgg.20180601.12,
  author = {Vladimir Boris Karpov},
  title = {3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope},
  journal = {International Journal of Genetics and Genomics},
  volume = {6},
  number = {1},
  pages = {8-10},
  doi = {10.11648/j.ijgg.20180601.12},
  url = {https://doi.org/10.11648/j.ijgg.20180601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20180601.12},
  abstract = {A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - 3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope
AU  - Vladimir Boris Karpov
Y1  - 2018/03/26
PY  - 2018
N1  - https://doi.org/10.11648/j.ijgg.20180601.12
DO  - 10.11648/j.ijgg.20180601.12
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 8
EP  - 10
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20180601.12
AB  - A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.
VL  - 6
IS  - 1
ER  -

Copy | Download