Manifestation of Functional Defects of Nervous System in <i>Upf3</i> Mutants <i>Drosophila melanogaster</i> at Larval and Adult Stages

Sanusi Ahmed Jega; Ahmed Adebowole Adedeji; Marta Vicente-Crespo

doi:doi:10.11648/j.ab.20200802.12

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Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages

Sanusi Ahmed Jega, Ahmed Adebowole Adedeji, Marta Vicente-Crespo

Received: 30 March 2020 Accepted: 13 April 2020 Published: 14 May 2020

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Abstract

Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that cleans the system from possible harmful proteins and also regulates up to 10% of normal RNAs. The essential player proteins in the NMD (core NMD factors) are Upf1, Upf2, and Upf3. Mutation of any of these NMD factors cause ranges of effects in the development of various organisms. In humans, mutation of Upf3 was associated with neurodegenerative disorders, which include: attention deficit, schizophrenia autism, and intellectual disability. Using functional genetics approach and behavioral analysis methods we examined the loss of function effects of Upf3, in the nervous system function of a Drosophila melanogaster. We observed certain nervous system functional defects in homozygous Upf3 mutants. The embryos exhibited reduced and delayed hatching, the larvae manifested defects in motor function and the adults showed reduced climbing ability, defective short term memory, and learning, and notably, the adult life span was also reduced. This work has further revealed the prospect of Upf3 as a player gene for consideration in the management of neurodegenerative diseases. We explored this using Drosophila melanogaster as a model organism to mimic and study the neurodegenerative traits observed in the patients suffering from Upf3 mutation. Likewise, it suggests a further investigation into the mechanistic insight for the roles of Upf3 in both early and late CNS development.

DOI	10.11648/j.ab.20200802.12
Published in	Advances in Biochemistry (Volume 8, Issue 2, June 2020)
Page(s)	38-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Nonsense-mediated mRNA Decay, NMD Factors, Organismal Development, Neurodegenerative Behaviors, Drosophila melanogaster

References

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APA Style

Sanusi Ahmed Jega, Ahmed Adebowole Adedeji, Marta Vicente-Crespo. (2020). Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages. Advances in Biochemistry, 8(2), 38-44. https://doi.org/10.11648/j.ab.20200802.12

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ACS Style

Sanusi Ahmed Jega; Ahmed Adebowole Adedeji; Marta Vicente-Crespo. Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages. Adv. Biochem. 2020, 8(2), 38-44. doi: 10.11648/j.ab.20200802.12

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AMA Style

Sanusi Ahmed Jega, Ahmed Adebowole Adedeji, Marta Vicente-Crespo. Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages. Adv Biochem. 2020;8(2):38-44. doi: 10.11648/j.ab.20200802.12

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@article{10.11648/j.ab.20200802.12,
  author = {Sanusi Ahmed Jega and Ahmed Adebowole Adedeji and Marta Vicente-Crespo},
  title = {Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages},
  journal = {Advances in Biochemistry},
  volume = {8},
  number = {2},
  pages = {38-44},
  doi = {10.11648/j.ab.20200802.12},
  url = {https://doi.org/10.11648/j.ab.20200802.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20200802.12},
  abstract = {Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that cleans the system from possible harmful proteins and also regulates up to 10% of normal RNAs. The essential player proteins in the NMD (core NMD factors) are Upf1, Upf2, and Upf3. Mutation of any of these NMD factors cause ranges of effects in the development of various organisms. In humans, mutation of Upf3 was associated with neurodegenerative disorders, which include: attention deficit, schizophrenia autism, and intellectual disability. Using functional genetics approach and behavioral analysis methods we examined the loss of function effects of Upf3, in the nervous system function of a Drosophila melanogaster. We observed certain nervous system functional defects in homozygous Upf3 mutants. The embryos exhibited reduced and delayed hatching, the larvae manifested defects in motor function and the adults showed reduced climbing ability, defective short term memory, and learning, and notably, the adult life span was also reduced. This work has further revealed the prospect of Upf3 as a player gene for consideration in the management of neurodegenerative diseases. We explored this using Drosophila melanogaster as a model organism to mimic and study the neurodegenerative traits observed in the patients suffering from Upf3 mutation. Likewise, it suggests a further investigation into the mechanistic insight for the roles of Upf3 in both early and late CNS development.},
 year = {2020}
}

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TY  - JOUR
T1  - Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages
AU  - Sanusi Ahmed Jega
AU  - Ahmed Adebowole Adedeji
AU  - Marta Vicente-Crespo
Y1  - 2020/05/14
PY  - 2020
N1  - https://doi.org/10.11648/j.ab.20200802.12
DO  - 10.11648/j.ab.20200802.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 38
EP  - 44
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20200802.12
AB  - Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that cleans the system from possible harmful proteins and also regulates up to 10% of normal RNAs. The essential player proteins in the NMD (core NMD factors) are Upf1, Upf2, and Upf3. Mutation of any of these NMD factors cause ranges of effects in the development of various organisms. In humans, mutation of Upf3 was associated with neurodegenerative disorders, which include: attention deficit, schizophrenia autism, and intellectual disability. Using functional genetics approach and behavioral analysis methods we examined the loss of function effects of Upf3, in the nervous system function of a Drosophila melanogaster. We observed certain nervous system functional defects in homozygous Upf3 mutants. The embryos exhibited reduced and delayed hatching, the larvae manifested defects in motor function and the adults showed reduced climbing ability, defective short term memory, and learning, and notably, the adult life span was also reduced. This work has further revealed the prospect of Upf3 as a player gene for consideration in the management of neurodegenerative diseases. We explored this using Drosophila melanogaster as a model organism to mimic and study the neurodegenerative traits observed in the patients suffering from Upf3 mutation. Likewise, it suggests a further investigation into the mechanistic insight for the roles of Upf3 in both early and late CNS development.
VL  - 8
IS  - 2
ER  -

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Author Information

Sanusi Ahmed Jega

Faculty of Biomedical Science, Kampala International University, Ishaka-Bushenyi, Uganda; Department of Biochemistry, Faculty of Life Sciences, Kebbi State University of Science and Technology, Aliero, Nigeria
Ahmed Adebowole Adedeji

Faculty of Biomedical Science, Kampala International University, Ishaka-Bushenyi, Uganda; Foresight Institute of Research and Translation, Ibadan, Nigeria
Marta Vicente-Crespo

Institute of Biomedical Research, Kampala International University, Ishaka-Bushenyi, Uganda; African Population and Health Research Center, Nairobi, Kenya

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Sanusi Ahmed Jega, Ahmed Adebowole Adedeji, Marta Vicente-Crespo. (2020). Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages. Advances in Biochemistry, 8(2), 38-44. https://doi.org/10.11648/j.ab.20200802.12

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ACS Style

Sanusi Ahmed Jega; Ahmed Adebowole Adedeji; Marta Vicente-Crespo. Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages. Adv. Biochem. 2020, 8(2), 38-44. doi: 10.11648/j.ab.20200802.12

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AMA Style

Sanusi Ahmed Jega, Ahmed Adebowole Adedeji, Marta Vicente-Crespo. Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages. Adv Biochem. 2020;8(2):38-44. doi: 10.11648/j.ab.20200802.12

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@article{10.11648/j.ab.20200802.12,
  author = {Sanusi Ahmed Jega and Ahmed Adebowole Adedeji and Marta Vicente-Crespo},
  title = {Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages},
  journal = {Advances in Biochemistry},
  volume = {8},
  number = {2},
  pages = {38-44},
  doi = {10.11648/j.ab.20200802.12},
  url = {https://doi.org/10.11648/j.ab.20200802.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20200802.12},
  abstract = {Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that cleans the system from possible harmful proteins and also regulates up to 10% of normal RNAs. The essential player proteins in the NMD (core NMD factors) are Upf1, Upf2, and Upf3. Mutation of any of these NMD factors cause ranges of effects in the development of various organisms. In humans, mutation of Upf3 was associated with neurodegenerative disorders, which include: attention deficit, schizophrenia autism, and intellectual disability. Using functional genetics approach and behavioral analysis methods we examined the loss of function effects of Upf3, in the nervous system function of a Drosophila melanogaster. We observed certain nervous system functional defects in homozygous Upf3 mutants. The embryos exhibited reduced and delayed hatching, the larvae manifested defects in motor function and the adults showed reduced climbing ability, defective short term memory, and learning, and notably, the adult life span was also reduced. This work has further revealed the prospect of Upf3 as a player gene for consideration in the management of neurodegenerative diseases. We explored this using Drosophila melanogaster as a model organism to mimic and study the neurodegenerative traits observed in the patients suffering from Upf3 mutation. Likewise, it suggests a further investigation into the mechanistic insight for the roles of Upf3 in both early and late CNS development.},
 year = {2020}
}

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TY  - JOUR
T1  - Manifestation of Functional Defects of Nervous System in Upf3 Mutants Drosophila melanogaster at Larval and Adult Stages
AU  - Sanusi Ahmed Jega
AU  - Ahmed Adebowole Adedeji
AU  - Marta Vicente-Crespo
Y1  - 2020/05/14
PY  - 2020
N1  - https://doi.org/10.11648/j.ab.20200802.12
DO  - 10.11648/j.ab.20200802.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 38
EP  - 44
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20200802.12
AB  - Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that cleans the system from possible harmful proteins and also regulates up to 10% of normal RNAs. The essential player proteins in the NMD (core NMD factors) are Upf1, Upf2, and Upf3. Mutation of any of these NMD factors cause ranges of effects in the development of various organisms. In humans, mutation of Upf3 was associated with neurodegenerative disorders, which include: attention deficit, schizophrenia autism, and intellectual disability. Using functional genetics approach and behavioral analysis methods we examined the loss of function effects of Upf3, in the nervous system function of a Drosophila melanogaster. We observed certain nervous system functional defects in homozygous Upf3 mutants. The embryos exhibited reduced and delayed hatching, the larvae manifested defects in motor function and the adults showed reduced climbing ability, defective short term memory, and learning, and notably, the adult life span was also reduced. This work has further revealed the prospect of Upf3 as a player gene for consideration in the management of neurodegenerative diseases. We explored this using Drosophila melanogaster as a model organism to mimic and study the neurodegenerative traits observed in the patients suffering from Upf3 mutation. Likewise, it suggests a further investigation into the mechanistic insight for the roles of Upf3 in both early and late CNS development.
VL  - 8
IS  - 2
ER  -

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