Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology
Tamara A. Ignatyeva,
Victor N. Voyevodin,
Anatoly N. Goltsev,
Victoria V. Kiroshka,
Alexander M. Bovda,
Valery V. Kalynovskii,
Alexey N. Velikodny,
Peter A. Kutsenko,
Vladimir Golub,
Yuri Dzhedzheria,
Irina Sharai
Issue:
Volume 2, Issue 6, December 2014
Pages:
72-77
Received:
18 December 2014
Accepted:
5 January 2015
Published:
14 January 2015
DOI:
10.11648/j.bio.20140206.11
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Abstract: Elastic hard magnetic materials based resin-bond magnets with the determined space configuration of the magnetic field required for a three-dimensional cell growth which is essential for the tissue engineering have been produced. Technical tests of the samples as well as the theoretical study of the distribution of stray fields produced by ferromagnetic particles correspondingly distributed in the film have been carried out. In vitro еxperimental investigations of the gradient magnetic field influence on a cell differentiation on transplanted epithelial-like kidney cells culture of a pig embryo has been carried out. It has been shown that the adhesion, morphology and proliferation rate of the cells is determined not only by the magnetic field value but also by its gradient direction. It has been established that the cell adhesion efficiency is the highest when the magnetic field gradient is directed from the Petri dish bottom to the air-culture medium interface. The obtained results prove the possibility of an implementation of new gradient magnetic fields based methods in biotechnology and in particular in tissue engineering.
Abstract: Elastic hard magnetic materials based resin-bond magnets with the determined space configuration of the magnetic field required for a three-dimensional cell growth which is essential for the tissue engineering have been produced. Technical tests of the samples as well as the theoretical study of the distribution of stray fields produced by ferromag...
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Plasma Protein Z Levels in Healthy and High-Risk Newborn Infants
Ahmed Anower Khattab,
Fathia Mohamed El Nemr,
Rania Salah El Zayat,
Mohmmed Soliman Rizk,
Shimaa Fetouh Elbakly
Issue:
Volume 2, Issue 6, December 2014
Pages:
78-82
Received:
24 November 2014
Accepted:
11 December 2014
Published:
21 January 2015
DOI:
10.11648/j.bio.20140206.12
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Abstract: Objectives: To evaluate plasma protein Z (PZ) levels in healthy and high-risk newborn infants. Background: Protein Z (PZ) is a vitamin K-dependent plasma protein , As is the case with other coagulation proteins and inhibitors, protein Z is consumed during disseminated intravascular coagulation (DIC), Functionally protein Z has been shown to be a direct requirement for the binding of thrombin to endothelial phospholipids , Protein Z also serves as a cofactor for the inhibition of coagulation factor Xa by a plasma serein called protein Z-dependent protease inhibitor (ZPI), The inhibitory function is exerted by the Protein Z- dependent protease inhibitor (ZPI), which circulates in the human plasma in a complex with PZ , The physiological function of protein Z is still rather ill-defined and may play role in high risk newborn. Methods: This study was conducted on 85 newborns divided in 4 groups ,(group I newborns affected by respiratory distress syndrome (RDS) , group II newborns from mothers with pre-eclampsia, group III newborns small for gestational age (SGA) and group IV healthy term and preterm newborns normal for gestational age. Newborns with sepsis, congenital malformation or hemorrhagic disorders were excluded, Plasma PZ levels was measured. Results: In the neonates of the study groups, protein z level was significant lower in patient group than control group, in group I ( 0.79 ±0.32), group II (0.70± 0.30), group III (0.78 ±0.32) and group IV (1.44 ±0.43) (p value<0.001). Conclusion: PZ deficiency occurs in newborns affected by severe RDS, in newborns from preeclampsic mothers and in SGA newborns, probably owing to activated coagulation in the first two conditions and to reduced PZ synthesis in the last one.
Abstract: Objectives: To evaluate plasma protein Z (PZ) levels in healthy and high-risk newborn infants. Background: Protein Z (PZ) is a vitamin K-dependent plasma protein , As is the case with other coagulation proteins and inhibitors, protein Z is consumed during disseminated intravascular coagulation (DIC), Functionally protein Z has been shown to be a di...
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