Advances in Biochemistry

Volume 3, Issue 6, December 2015

  • "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics

    Oleg Gradov, Margaret Gradova

    Issue: Volume 3, Issue 6, December 2015
    Pages: 66-71
    Received: 26 August 2015
    Accepted: 09 October 2015
    Published: 30 October 2015
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    Abstract: In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scan... Show More
  • 2-(2-(Tert-Butoxycarbonyl) Propanamido) Acetic Acid ‘NCA-AG’ Synthesis, Characterization and Reaction Optimization for the Preparation

    Estella Judith Salamula, Martin Bredenkamp, Deon Bezuidenhout, Misael Silas Nadiye-Tabbiruka

    Issue: Volume 3, Issue 6, December 2015
    Pages: 72-76
    Received: 06 October 2015
    Accepted: 23 October 2015
    Published: 10 November 2015
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    Abstract: 2-(2-(tert-butoxycarbonyl) propanamido) acetic acid (NCA-AG) was for the first time prepared using a variation of the Leuchs method. All compounds were characterized using melting point, nuclear magnetic resonance (NMR), ultra violet spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR) and electron spray mass spectroscopy (ESMS). The r... Show More
  • Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

    Issue: Volume 3, Issue 6, December 2015
    Pages: 77-85
    Received: 08 October 2015
    Accepted: 16 October 2015
    Published: 16 November 2015
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    Abstract: SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as ... Show More
  • Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

    Daphne Santoro Leonardi-Carvalho, Sergio Zucoloto, Paula Payão Ovidio, Renato Heidor, Thomas Prates Ong, Fernando Salvador Moreno, Alceu Afonso Jordao

    Issue: Volume 3, Issue 6, December 2015
    Pages: 86-95
    Received: 31 August 2015
    Accepted: 29 October 2015
    Published: 10 December 2015
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    Abstract: The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of la... Show More
  • Isotopic Mass-Dependent MS-Patch-Clamp and Isotopic Mass-Independent ESR-Patch-Clamp

    Serge Pankratov

    Issue: Volume 3, Issue 6, December 2015
    Pages: 96-112
    Received: 04 September 2015
    Accepted: 17 November 2015
    Published: 30 December 2015
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    Abstract: This paper is a comprehensive review of the possibilities of isotopic patch-clamp and isotopic mass-patch-clamp technique development and represents a brief summary of the student's tutorial review by S. Pankratov. We propose to distinguish between the mass-dependent and mass-independent patch-clamp. The main principles introduced here will be deve... Show More