The sterile male technique (SIT), an autocidal, biological technique that respects the environment, is an efficient tool for tsetse flies eradication. This method requires many sterilized male flies released for mating with wild virgin female flies. The mass production of male flies by rearing in the laboratory and the supply of pupae is becoming more and more difficult. Obtaining sterile males in quality and quantity remains an obstacle to biological control. The aim of this study was to determine the behavior of wild tsetse flies collected in the field for rearing in the insectary to set up a laboratory colony in Mali. Collection was done in the natural habitat nearby the river in the surrounding area of Bamako by using biconical traps set from 06 a.m. to 04 p.m. the following day. Climatic and geographical parameters were recorded. Collected flies have been transferred into a cooler chamber for transportation to the insectary at the Laboratory of Entomology and Parasitology of the Faculty of Sciences and Technics (FST) of the University of Sciences, Technics and Technologies of Bamako (USTTB). Daily monitoring showed that mortality in the 72 hours post-collection varied from 10.4% to 100%. The average survival rate was estimated to 11.28% composed by 6.67% males and 4.61% females. None of them survived after 7 days in the insectary. This fact suggests that the adaptation of wild flies to the insectary needs to be further investigated to make it possible. Meanwhile, the use of pupae to set up a colony or the transfer of flies from another colony seems to be more efficient for the setting of a new colony.
| Published in | Science Research (Volume 11, Issue 5) |
| DOI | 10.11648/j.sr.20231105.15 |
| Page(s) | 123-128 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Wild Tsetse, Rearing, Stress, Adaptation Issue
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APA Style
Traore, A., Koné, O. I., Bass, B., Ly, B., Kounta, C. A., et al. (2023). Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali. Science Research, 11(5), 123-128. https://doi.org/10.11648/j.sr.20231105.15
ACS Style
Traore, A.; Koné, O. I.; Bass, B.; Ly, B.; Kounta, C. A., et al. Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali. Sci. Res. 2023, 11(5), 123-128. doi: 10.11648/j.sr.20231105.15
AMA Style
Traore A, Koné OI, Bass B, Ly B, Kounta CA, et al. Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali. Sci Res. 2023;11(5):123-128. doi: 10.11648/j.sr.20231105.15
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Download@article{10.11648/j.sr.20231105.15,
author = {Astan Traore and Ousmane Ibrahim Koné and Boubacar Bass and Bintou Ly and Cheick Abou Kounta and Youssouf Faya Kéita and Ambielè Bernard Sodio and Alpha Seydou Yaro},
title = {Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali},
journal = {Science Research},
volume = {11},
number = {5},
pages = {123-128},
doi = {10.11648/j.sr.20231105.15},
url = {https://doi.org/10.11648/j.sr.20231105.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sr.20231105.15},
abstract = {The sterile male technique (SIT), an autocidal, biological technique that respects the environment, is an efficient tool for tsetse flies eradication. This method requires many sterilized male flies released for mating with wild virgin female flies. The mass production of male flies by rearing in the laboratory and the supply of pupae is becoming more and more difficult. Obtaining sterile males in quality and quantity remains an obstacle to biological control. The aim of this study was to determine the behavior of wild tsetse flies collected in the field for rearing in the insectary to set up a laboratory colony in Mali. Collection was done in the natural habitat nearby the river in the surrounding area of Bamako by using biconical traps set from 06 a.m. to 04 p.m. the following day. Climatic and geographical parameters were recorded. Collected flies have been transferred into a cooler chamber for transportation to the insectary at the Laboratory of Entomology and Parasitology of the Faculty of Sciences and Technics (FST) of the University of Sciences, Technics and Technologies of Bamako (USTTB). Daily monitoring showed that mortality in the 72 hours post-collection varied from 10.4% to 100%. The average survival rate was estimated to 11.28% composed by 6.67% males and 4.61% females. None of them survived after 7 days in the insectary. This fact suggests that the adaptation of wild flies to the insectary needs to be further investigated to make it possible. Meanwhile, the use of pupae to set up a colony or the transfer of flies from another colony seems to be more efficient for the setting of a new colony.},
year = {2023}
}
TY - JOUR T1 - Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali AU - Astan Traore AU - Ousmane Ibrahim Koné AU - Boubacar Bass AU - Bintou Ly AU - Cheick Abou Kounta AU - Youssouf Faya Kéita AU - Ambielè Bernard Sodio AU - Alpha Seydou Yaro Y1 - 2023/10/14 PY - 2023 N1 - https://doi.org/10.11648/j.sr.20231105.15 DO - 10.11648/j.sr.20231105.15 T2 - Science Research JF - Science Research JO - Science Research SP - 123 EP - 128 PB - Science Publishing Group SN - 2329-0927 UR - https://doi.org/10.11648/j.sr.20231105.15 AB - The sterile male technique (SIT), an autocidal, biological technique that respects the environment, is an efficient tool for tsetse flies eradication. This method requires many sterilized male flies released for mating with wild virgin female flies. The mass production of male flies by rearing in the laboratory and the supply of pupae is becoming more and more difficult. Obtaining sterile males in quality and quantity remains an obstacle to biological control. The aim of this study was to determine the behavior of wild tsetse flies collected in the field for rearing in the insectary to set up a laboratory colony in Mali. Collection was done in the natural habitat nearby the river in the surrounding area of Bamako by using biconical traps set from 06 a.m. to 04 p.m. the following day. Climatic and geographical parameters were recorded. Collected flies have been transferred into a cooler chamber for transportation to the insectary at the Laboratory of Entomology and Parasitology of the Faculty of Sciences and Technics (FST) of the University of Sciences, Technics and Technologies of Bamako (USTTB). Daily monitoring showed that mortality in the 72 hours post-collection varied from 10.4% to 100%. The average survival rate was estimated to 11.28% composed by 6.67% males and 4.61% females. None of them survived after 7 days in the insectary. This fact suggests that the adaptation of wild flies to the insectary needs to be further investigated to make it possible. Meanwhile, the use of pupae to set up a colony or the transfer of flies from another colony seems to be more efficient for the setting of a new colony. VL - 11 IS - 5 ER -
Laboratory of Entomology-Parasitology, Department of Biology, Faculty of Sciences and Techniques (FST), University of Sciences, Techniques and Technologies of Bamako (USTT-B), Hill of Badalabougou, Bamako, Mali
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