Microscopic Study of the Major Plant Part of a Euphorbia, <i>Croton bonplandianum</i> Baill

Rubaiyat Sharmin Sultana

doi:doi:10.11648/j.sf.20220304.11

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Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill

Rubaiyat Sharmin Sultana

Published in Science Frontiers (Volume 3, Issue 4)

Received: 12 October 2022 Accepted: 29 October 2022 Published: 10 November 2022

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Abstract

Croton bonplandianum Baill. is an herbaceous medicinal plant, and was taken for anatomical study in the present investigation. Prime parts, i.e., stem, leaf, and root, were studied anatomically in free-hand sections after being stained with 1% safranin under a light microscope. The micrographs were taken with an accessory digital camera, and the diameter was measured from the micrographs. The stem was angular in cross-cut view and consisted of an epidermal layer including a layer of spindle-shaped cells (22.3mm). The cortex had seven to eight layers of elliptical, thick-walled compact parenchyma cells (43.5mm). The secondary xylem in the vascular bundle was endarch and thick-walled. Secondary phloem grew in a continuous cylinder around the xylem. Pith was wide, composed of parenchymatous cells, and 85.7 mm in diameter. The transverse section of the leaf showed the isobilateral arrangement. The epidermal layer was made up of a single layer of compressed to elongated parenchymatous cells. The horizontal palisade layer was present in the adaxial sub epidermal part of the midrib. The midrib was represented by a vascular bundle which was made up of phloem (outer) and xylem (inner). Calcium oxalate crystals were seen in phloem parenchyma. The crystal layer was one or two cells thick. A sclerid layer was seen around the vascular bundle. The surface preparation showed the paracytic type of stomata. Number of stomata was 0.227/mm² and the index was 21.5. The transverse section of the root was composed of the outer epidermis, which was made up of a few layers of irregular and compressed cork cells. Sclerenchymatous cells were also found to be scattered throughout the cortex and were lignified in nature. The vascular bundle was collateral in nature, with phloem arranged outwards and the xylem (lignified, 28.3mm in diameter) situated inwards, occupying the whole area of the section. The secondary phloem was well preserved. It included parenchyma cells and sieve elements associated with the companion cells. The central pith was found to be absent.

Published in	Science Frontiers (Volume 3, Issue 4)
DOI	10.11648/j.sf.20220304.11
Page(s)	134-139
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Croton bonplandianum, Flowering Plant, Leaf, Plant Anatomy, Root, Stem

References

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Rubaiyat Sharmin Sultana. (2022). Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill. Science Frontiers, 3(4), 134-139. https://doi.org/10.11648/j.sf.20220304.11

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ACS Style

Rubaiyat Sharmin Sultana. Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill. Sci. Front. 2022, 3(4), 134-139. doi: 10.11648/j.sf.20220304.11

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AMA Style

Rubaiyat Sharmin Sultana. Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill. Sci Front. 2022;3(4):134-139. doi: 10.11648/j.sf.20220304.11

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@article{10.11648/j.sf.20220304.11,
  author = {Rubaiyat Sharmin Sultana},
  title = {Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill},
  journal = {Science Frontiers},
  volume = {3},
  number = {4},
  pages = {134-139},
  doi = {10.11648/j.sf.20220304.11},
  url = {https://doi.org/10.11648/j.sf.20220304.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sf.20220304.11},
  abstract = {Croton bonplandianum Baill. is an herbaceous medicinal plant, and was taken for anatomical study in the present investigation. Prime parts, i.e., stem, leaf, and root, were studied anatomically in free-hand sections after being stained with 1% safranin under a light microscope. The micrographs were taken with an accessory digital camera, and the diameter was measured from the micrographs. The stem was angular in cross-cut view and consisted of an epidermal layer including a layer of spindle-shaped cells (22.3mm). The cortex had seven to eight layers of elliptical, thick-walled compact parenchyma cells (43.5mm). The secondary xylem in the vascular bundle was endarch and thick-walled. Secondary phloem grew in a continuous cylinder around the xylem. Pith was wide, composed of parenchymatous cells, and 85.7 mm in diameter. The transverse section of the leaf showed the isobilateral arrangement. The epidermal layer was made up of a single layer of compressed to elongated parenchymatous cells. The horizontal palisade layer was present in the adaxial sub epidermal part of the midrib. The midrib was represented by a vascular bundle which was made up of phloem (outer) and xylem (inner). Calcium oxalate crystals were seen in phloem parenchyma. The crystal layer was one or two cells thick. A sclerid layer was seen around the vascular bundle. The surface preparation showed the paracytic type of stomata. Number of stomata was 0.227/mm2 and the index was 21.5. The transverse section of the root was composed of the outer epidermis, which was made up of a few layers of irregular and compressed cork cells. Sclerenchymatous cells were also found to be scattered throughout the cortex and were lignified in nature. The vascular bundle was collateral in nature, with phloem arranged outwards and the xylem (lignified, 28.3mm in diameter) situated inwards, occupying the whole area of the section. The secondary phloem was well preserved. It included parenchyma cells and sieve elements associated with the companion cells. The central pith was found to be absent.},
 year = {2022}
}

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TY - JOUR
T1 - Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill
AU - Rubaiyat Sharmin Sultana
Y1 - 2022/11/10
PY - 2022
N1 - https://doi.org/10.11648/j.sf.20220304.11
DO - 10.11648/j.sf.20220304.11
T2 - Science Frontiers
JF - Science Frontiers
JO - Science Frontiers
SP - 134
EP - 139
PB - Science Publishing Group
SN - 2994-7030
UR - https://doi.org/10.11648/j.sf.20220304.11
AB - Croton bonplandianum Baill. is an herbaceous medicinal plant, and was taken for anatomical study in the present investigation. Prime parts, i.e., stem, leaf, and root, were studied anatomically in free-hand sections after being stained with 1% safranin under a light microscope. The micrographs were taken with an accessory digital camera, and the diameter was measured from the micrographs. The stem was angular in cross-cut view and consisted of an epidermal layer including a layer of spindle-shaped cells (22.3mm). The cortex had seven to eight layers of elliptical, thick-walled compact parenchyma cells (43.5mm). The secondary xylem in the vascular bundle was endarch and thick-walled. Secondary phloem grew in a continuous cylinder around the xylem. Pith was wide, composed of parenchymatous cells, and 85.7 mm in diameter. The transverse section of the leaf showed the isobilateral arrangement. The epidermal layer was made up of a single layer of compressed to elongated parenchymatous cells. The horizontal palisade layer was present in the adaxial sub epidermal part of the midrib. The midrib was represented by a vascular bundle which was made up of phloem (outer) and xylem (inner). Calcium oxalate crystals were seen in phloem parenchyma. The crystal layer was one or two cells thick. A sclerid layer was seen around the vascular bundle. The surface preparation showed the paracytic type of stomata. Number of stomata was 0.227/mm2 and the index was 21.5. The transverse section of the root was composed of the outer epidermis, which was made up of a few layers of irregular and compressed cork cells. Sclerenchymatous cells were also found to be scattered throughout the cortex and were lignified in nature. The vascular bundle was collateral in nature, with phloem arranged outwards and the xylem (lignified, 28.3mm in diameter) situated inwards, occupying the whole area of the section. The secondary phloem was well preserved. It included parenchyma cells and sieve elements associated with the companion cells. The central pith was found to be absent.
VL - 3
IS - 4
ER -

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Rubaiyat Sharmin Sultana

Department of Botany, University of Rajshahi, Rajshahi, Bangladesh

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Rubaiyat Sharmin Sultana. (2022). Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill. Science Frontiers, 3(4), 134-139. https://doi.org/10.11648/j.sf.20220304.11

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ACS Style

Rubaiyat Sharmin Sultana. Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill. Sci. Front. 2022, 3(4), 134-139. doi: 10.11648/j.sf.20220304.11

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AMA Style

Rubaiyat Sharmin Sultana. Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill. Sci Front. 2022;3(4):134-139. doi: 10.11648/j.sf.20220304.11

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@article{10.11648/j.sf.20220304.11,
  author = {Rubaiyat Sharmin Sultana},
  title = {Microscopic Study of the Major Plant Part of a Euphorbia, Croton bonplandianum Baill},
  journal = {Science Frontiers},
  volume = {3},
  number = {4},
  pages = {134-139},
  doi = {10.11648/j.sf.20220304.11},
  url = {https://doi.org/10.11648/j.sf.20220304.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sf.20220304.11},
  abstract = {Croton bonplandianum Baill. is an herbaceous medicinal plant, and was taken for anatomical study in the present investigation. Prime parts, i.e., stem, leaf, and root, were studied anatomically in free-hand sections after being stained with 1% safranin under a light microscope. The micrographs were taken with an accessory digital camera, and the diameter was measured from the micrographs. The stem was angular in cross-cut view and consisted of an epidermal layer including a layer of spindle-shaped cells (22.3mm). The cortex had seven to eight layers of elliptical, thick-walled compact parenchyma cells (43.5mm). The secondary xylem in the vascular bundle was endarch and thick-walled. Secondary phloem grew in a continuous cylinder around the xylem. Pith was wide, composed of parenchymatous cells, and 85.7 mm in diameter. The transverse section of the leaf showed the isobilateral arrangement. The epidermal layer was made up of a single layer of compressed to elongated parenchymatous cells. The horizontal palisade layer was present in the adaxial sub epidermal part of the midrib. The midrib was represented by a vascular bundle which was made up of phloem (outer) and xylem (inner). Calcium oxalate crystals were seen in phloem parenchyma. The crystal layer was one or two cells thick. A sclerid layer was seen around the vascular bundle. The surface preparation showed the paracytic type of stomata. Number of stomata was 0.227/mm2 and the index was 21.5. The transverse section of the root was composed of the outer epidermis, which was made up of a few layers of irregular and compressed cork cells. Sclerenchymatous cells were also found to be scattered throughout the cortex and were lignified in nature. The vascular bundle was collateral in nature, with phloem arranged outwards and the xylem (lignified, 28.3mm in diameter) situated inwards, occupying the whole area of the section. The secondary phloem was well preserved. It included parenchyma cells and sieve elements associated with the companion cells. The central pith was found to be absent.},
 year = {2022}
}

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