Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology

Liu Yan; Dai Xujie

doi:doi:10.11648/j.sd.20170505.11

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Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology

Liu Yan, Dai Xujie

Published in Science Discovery (Volume 5, Issue 5)

Received: 19 July 2017 Accepted: Published: 19 July 2017

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Abstract

Kombucha cellulose is a kind of secondary metabolites produced by microorganisms. It is a kind of extracellular polysaccharide which is linked with β-1,4 glycosides. Compared with lignocellulose, it is the key focus of the current research in society, with a complex three dimensional reticular structure,material-combining ability, good biocompatibility, high purity, outstanding water-holding capacity, whichcan play an important role in many areas. Optimization of culture medium by response surface method to determine the optimum Kombucha culture conditions. The optimum culture condition was: Black Tea 2.16g, sucrose 27.33g, bran 4.35g, inoculum 23.03%,culture 48h, under these conditions, the growth of biofilm thickness of Kombucha can reach 0.76cm. At the same time, the growth rate and the establishment of Kombucha Black Tea between sucrose, wheat bran and the inoculation amount of two polynomial model, the model can predict the growth status of Kombucha, can obtain the optimal conditions.

Published in	Science Discovery (Volume 5, Issue 5)
DOI	10.11648/j.sd.20170505.11
Page(s)	307-311
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Kombucha Cellulose, Optimization, Culture Conditions, Response Surface

References

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[2]	朱清梅，林强，冯玉红，等．细菌纤维素基材料的制备及其应用前景[J]．海南大学学报：自然科学版，2010, 28(2):163-167.
[3]	陈竞，冯蕾，杨新平．细菌纤维素的制备和应用研究进展[J]．纤维素科学与技术，2014，22(2):58-63。
[4]	Iguchi M, Yamanaka S, Budhiono A. Bacterial cellulose—a masterpiece of nature's arts [J]. Journal ofMaterials Science, 2000, 35(2): 261-270.
[5]	武志芳，张霞，易彬，等．新型生物材料细菌纤维素的研究进展[J]．食品与发酵科技，2010（1）:27-30。
[6]	Padrão J, Gonçalves S, Silva J P, et al. Bacterial cellulose-lactoferrin as an antimicrobial ediblepackaging [J]. Food Hydrocolloids, 2016, 58：126-140.
[7]	许如清．细菌纤维素基振膜材料的声学性能研究[D]．东华大学，2012。
[8]	刘艳，冯印等．响应面法优化产虾青素假单胞菌的发酵培养基[D]．食品工业科技，2014（20）186-190。
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Liu Yan, Dai Xujie. (2017). Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology. Science Discovery, 5(5), 307-311. https://doi.org/10.11648/j.sd.20170505.11

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ACS Style

Liu Yan; Dai Xujie. Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology. Sci. Discov. 2017, 5(5), 307-311. doi: 10.11648/j.sd.20170505.11

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AMA Style

Liu Yan, Dai Xujie. Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology. Sci Discov. 2017;5(5):307-311. doi: 10.11648/j.sd.20170505.11

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@article{10.11648/j.sd.20170505.11,
  author = {Liu Yan and Dai Xujie},
  title = {Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology},
  journal = {Science Discovery},
  volume = {5},
  number = {5},
  pages = {307-311},
  doi = {10.11648/j.sd.20170505.11},
  url = {https://doi.org/10.11648/j.sd.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20170505.11},
  abstract = {Kombucha cellulose is a kind of secondary metabolites produced by microorganisms. It is a kind of extracellular polysaccharide which is linked with β-1,4 glycosides. Compared with lignocellulose, it is the key focus of the current research in society, with a complex three dimensional reticular structure,material-combining ability, good biocompatibility, high purity, outstanding water-holding capacity, whichcan play an important role in many areas. Optimization of culture medium by response surface method to determine the optimum Kombucha culture conditions. The optimum culture condition was: Black Tea 2.16g, sucrose 27.33g, bran 4.35g, inoculum 23.03%,culture 48h, under these conditions, the growth of biofilm thickness of Kombucha can reach 0.76cm. At the same time, the growth rate and the establishment of Kombucha Black Tea between sucrose, wheat bran and the inoculation amount of two polynomial model, the model can predict the growth status of Kombucha, can obtain the optimal conditions.},
 year = {2017}
}

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TY  - JOUR
T1  - Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology
AU  - Liu Yan
AU  - Dai Xujie
Y1  - 2017/07/19
PY  - 2017
N1  - https://doi.org/10.11648/j.sd.20170505.11
DO  - 10.11648/j.sd.20170505.11
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 307
EP  - 311
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20170505.11
AB  - Kombucha cellulose is a kind of secondary metabolites produced by microorganisms. It is a kind of extracellular polysaccharide which is linked with β-1,4 glycosides. Compared with lignocellulose, it is the key focus of the current research in society, with a complex three dimensional reticular structure,material-combining ability, good biocompatibility, high purity, outstanding water-holding capacity, whichcan play an important role in many areas. Optimization of culture medium by response surface method to determine the optimum Kombucha culture conditions. The optimum culture condition was: Black Tea 2.16g, sucrose 27.33g, bran 4.35g, inoculum 23.03%,culture 48h, under these conditions, the growth of biofilm thickness of Kombucha can reach 0.76cm. At the same time, the growth rate and the establishment of Kombucha Black Tea between sucrose, wheat bran and the inoculation amount of two polynomial model, the model can predict the growth status of Kombucha, can obtain the optimal conditions.
VL  - 5
IS  - 5
ER  -

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Author Information

Liu Yan

College of Biological Foods, Changchun University of Science and Technology, Changchun, China
Dai Xujie

College of Biological Foods, Changchun University of Science and Technology, Changchun, China

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APA Style

Liu Yan, Dai Xujie. (2017). Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology. Science Discovery, 5(5), 307-311. https://doi.org/10.11648/j.sd.20170505.11

Copy | Download

ACS Style

Liu Yan; Dai Xujie. Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology. Sci. Discov. 2017, 5(5), 307-311. doi: 10.11648/j.sd.20170505.11

Copy | Download

AMA Style

Liu Yan, Dai Xujie. Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology. Sci Discov. 2017;5(5):307-311. doi: 10.11648/j.sd.20170505.11

Copy | Download

@article{10.11648/j.sd.20170505.11,
  author = {Liu Yan and Dai Xujie},
  title = {Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology},
  journal = {Science Discovery},
  volume = {5},
  number = {5},
  pages = {307-311},
  doi = {10.11648/j.sd.20170505.11},
  url = {https://doi.org/10.11648/j.sd.20170505.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20170505.11},
  abstract = {Kombucha cellulose is a kind of secondary metabolites produced by microorganisms. It is a kind of extracellular polysaccharide which is linked with β-1,4 glycosides. Compared with lignocellulose, it is the key focus of the current research in society, with a complex three dimensional reticular structure,material-combining ability, good biocompatibility, high purity, outstanding water-holding capacity, whichcan play an important role in many areas. Optimization of culture medium by response surface method to determine the optimum Kombucha culture conditions. The optimum culture condition was: Black Tea 2.16g, sucrose 27.33g, bran 4.35g, inoculum 23.03%,culture 48h, under these conditions, the growth of biofilm thickness of Kombucha can reach 0.76cm. At the same time, the growth rate and the establishment of Kombucha Black Tea between sucrose, wheat bran and the inoculation amount of two polynomial model, the model can predict the growth status of Kombucha, can obtain the optimal conditions.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology
AU  - Liu Yan
AU  - Dai Xujie
Y1  - 2017/07/19
PY  - 2017
N1  - https://doi.org/10.11648/j.sd.20170505.11
DO  - 10.11648/j.sd.20170505.11
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 307
EP  - 311
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20170505.11
AB  - Kombucha cellulose is a kind of secondary metabolites produced by microorganisms. It is a kind of extracellular polysaccharide which is linked with β-1,4 glycosides. Compared with lignocellulose, it is the key focus of the current research in society, with a complex three dimensional reticular structure,material-combining ability, good biocompatibility, high purity, outstanding water-holding capacity, whichcan play an important role in many areas. Optimization of culture medium by response surface method to determine the optimum Kombucha culture conditions. The optimum culture condition was: Black Tea 2.16g, sucrose 27.33g, bran 4.35g, inoculum 23.03%,culture 48h, under these conditions, the growth of biofilm thickness of Kombucha can reach 0.76cm. At the same time, the growth rate and the establishment of Kombucha Black Tea between sucrose, wheat bran and the inoculation amount of two polynomial model, the model can predict the growth status of Kombucha, can obtain the optimal conditions.
VL  - 5
IS  - 5
ER  -

Copy | Download