| Peer-Reviewed

Activation of Cotyledon as Nutrients and Antioxidant Enhancer in Oryza sativa, Dioscorea bulbifera and Phaseolus vulgaris for Food Formulation

Received: 28 December 2021    Accepted: 18 February 2022    Published: 28 February 2022
Views:       Downloads:
Abstract

The impact of cotyledon activation on brown rice Rice (Oryza sativa), Aerial yam (Dioscorea bulbifera) and black turtle bean (Phaseolus vulgaris) was studied. Composite flours were prepared using cotyledon activated brown rice (GBR), aerial yam (AYF) and black turtle bean (GTB). The ratio of GBR: AYF: GTB used in the flour formulations were: 100GBR: 0AYF: 0GTB; 0GBR: 100AYF: 0GTB; 0GBR: 0AYF: 100GTB; 33.33GBR: 33.33AYF: 33.33GTB; 66.67GBR: 16.67AYF: 16.67GTB, 16.67GBR: 66.67AYF: 16.67GTB and 16.67GBR: 16.67AYF: 66.67GTB. Proximate composition, functional properties, antinutrients, minerals, vitamins, amino acid profile and antioxidant activity of the composite flours were determined using standard procedures. Protein efficiency ratio, essential amino acid index and biological value were calculated from the amino acid profile. Results showed ash content (2.72-3.20%), fibre (2.12-2.42%), protein (13.23-39.31%), tannin (0.973-1.120mg/100g), saponin (1.95-2.44 mg/g), oxalate (2.50-3.38mg/g), water absorption capacity (107.71–190.23%), oil absorption capacity (145.30-296.70%), water solubility index (16.05-17.78%), swelling capacity (230.10-250.50%), calcium (36.64-40.62mg/100g), phosphorus (84.84-88.93mg/100g), manganese (0.46-0.57mg/100g), magnesium (1741.20-1823.20mg/100g), vitamin B1 (2.75-4.06mg/100g, B2 (2.01-2.78 mg/100g, B3 (2.20-4.83mg/100g), B6 (2.63-3.98mg/100g) B12 (1.59-5.95mg/100g), essential amino acid index (70-99%), protein efficiency ratio (1.95–3.79), and biological value (70-96%). Nutrients and antioxidant properties were improved through the cotyledon activation process, level of antinutrients were safe and below toxicity level. The composite flour is invaluable as functional ingredient in food formulation.

Published in Science Development (Volume 3, Issue 1)
DOI 10.11648/j.scidev.20220301.15
Page(s) 31-45
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Cotyledon Activation, Nutritional, Antioxidants, Amino Acid, Brown Rice, Aerial Yam, Black Turtle Bean

References
[1] Hasmadi, M., Noorfarahzilah, M., Noraidah, H., Zainol, M. K. and Jahurul, M. H. A. (2020). Functional properties of composite flour: a review. Food Research 4 (6): 1820–1831.
[2] Igbabul, B. D., Iorliam, B. M., and Umana, E. N. (2015) Physicochemical and sensory properties of cookies produced from composite flours of wheat, cocoyam and African yam beans. Journal of Food Research. 4 (2): 150-151.
[3] FAO/WHO (2001). Human vitamin and mineral requirements. Report of a Joint FAO/WHO Expert Consultation, Food and Nutrition Division, Bangkok, Thailand: 7-8.
[4] Ebuehi, O. A. T., and Oyewole, A. C. (2008). Effect of cooking and soaking on physical, nutrient composition and sensory evaluation of indigenous and foreign rice varieties in Nigeria. Nutrition and Food Science, 38 (1): 15-21.
[5] Bryant, R. J. Kadan, R. S., Champagne, E. J., Veinyord, B. T. and Boykin, B. B. (2001). Functional and Digestive Characteristics of Extruded Rice Flour. Cereal Chemistry 78: 131-137.
[6] Gujral, H. S and Rosell, C. M (2004) Functionality of rice flour modified by microbial transglutaminase. Journal of Cereal Science 39 (2): 225-230.
[7] Falade, K. O., and Christopher, A. S. (2014). Physical, functional, pasting and thermal properties of flours and starches of six Nigerian rice cultivars. Food Hydrocolloids, 39: 41-50.
[8] Wu, F. F., Yang, N., Toure, A., Jin, Z. Y and Xu, X. M. (2013). Germinated brown rice and its role in human health. Crit Rev Food Sci Nutr, 53 (5): 451–463.
[9] Cornejo, F., Caceres, P. J., Martínez-Villaluenga, C., Rosell, C. M., and Frias, J. (2015). Effects of germination on the nutritive value and bioactive compounds of brown rice breads. Food Chemistry, 173, 298-304.
[10] Awolu, O. O., Morayo, P. O., Iyanuoluwa, O. F. and Funmilayo, G. O (2015) Optimization of the extrusion process for the production of readyto-eat snack from rice, cassava and Kersting’s groundnut composite flours. LWT-Food Sci Technol 64: 19–24. doi: 10.1016/j.lwt.2015. 05.025.
[11] Awolu, O. O., Omoba, O. S., Olawoye, O., and Dairo, M. (2017). Optimization of production and quality evaluation of maizebased snack supplemented with soybean and tiger-nut (Cyperus esculenta) flour. Food Science & Nutrition. 5 (1), 3–13.
[12] Amoldi, A., Zanoni, C., Lammi, C., and Boschin, G. (2014). The role of grain legumes in the prevention of hypercholesterolemia and hypertension. Critical Review in Plant Sciences, Vol. 34; Issue 1-3, p. 105-143.
[13] Boelt, B., Julier, B., karagic, D. and Hampton, J. (2014). Legume seed production meeting market requirements and economic impacts. Critical Reviews in Plant Sciences, Vol 34; Issue 1-3 p. 95-103.
[14] Okafor, D. C., Enwereuzoh, R. O., Ibeabuchi, J. C., Uzoukwu, A. E., Alagboso, S. O and Udenkwo, C (2015). Production of Flour types from black bean (Phaseolus vulgaris) and effect of pH and temperature on functional physio-chemical properties of the flours. European journal of Food Science and Technology. 3 (2): 64-84.
[15] Chima, J. U and Fasuan, T. O (2020) Antioxidant, nutritional, antinutrients and functional characteristics of black turtle bean (Phaseolus vulgaris): synergistic and antagonistic interrelationship of epigeal germination periods. https://www.emerald.com/insight/0034-6659.htm.
[16] Aremu, M. O., Audu, S. S., and Gav, B. L (2017). Comparative Review of Crude Protein and Amino Acid Composition of Some Leguminous Seeds Grown in Nigeria. International Journal of Sciences. 6 (8): 89-97.
[17] Reverri, E. J; Randolph, J. M; Steinberg, F. M; Tissa Kappagoda, C; Edirisinghe. I and Burton-Freeman, B. M (2015) Black Beans, Fiber, and Antioxidant Capacity Pilot Study: Examination of Whole Foods vs. Functional Components on Postprandial Metabolic, Oxidative Stress, and Inflammation in Adults with Metabolic Syndrome. Nutrients 7, 6139-6154; doi: 10.3390/nu7085273.
[18] Hayat, I., Ahmad, A., Masud, T., Ahmed, A., & Bashir, S. (2014). Nutritional and health perspectives of beans (Phaseolus vulgaris L.): an overview. Critical Reviews in Food Science and Nutrition, 54 (5), 580–92.
[19] Lawal, A. I and Akinoso. R (2019). Physical Properties, Proximate Composition and Antioxidant Activities of Aerial Yam (Dioscorea bulbifera) Bulbils Grown in Nigeria. BIBLID: 1450-7188. 50, 143-151.
[20] Olatoye, K. K and Aruenya, G. L (2019) Nutrient and phytochemical composition of flour made from selected cultivars of Aerial yam (Dioscorea bulbifera) in Nigeria. Journal of Food Composition and Analysis 79: 23–27.
[21] Aathira M and Siddhuraju P. (2017). Evaluation on suitability of differentially processed D. bulbifera tubers (Aerial and Underground) as alternative in composite flours for future food innovations. International Journal of Food Science and Nutrition.; 2 (6): 142-148.
[22] Ojinnaka, M. C., Odimegwu, E. N., and Ilechukwu, R. (2016) Functional properties of flour and starch from two cultivars of aerial yam (Dioscorea bulbifera) in South East Nigeria. IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-ISSN: 2319-2380, p-ISSN: 2319-2372. Volume 9, Issue 8 Ver. I: 22-25.
[23] AOAC (2005). Official Methods of Analysis, 18th ed. Association of official Analytical chemists Washington, D.C. USA.
[24] Brunner J. H (1994). “Direct Spectrosphotometric Determination of Saponin”. Journal. Analytical Chemistry. 34: 1314-1326.
[25] Falade O. S, Dare A. F, Bello M. O, Osuntogun B. O, and Adewusi S. R. A (2004). “Varietal Changes in Proximate composition and the Effect of Processing on the ascorbic content of some Nigerian Vegetables”. Journal of Food Technology 2: 103-108.
[26] Vaintraub, I. A. and Lapteva, N. A. (1988). “Colorimetric determination of phytate in unpurified extracts of seeds, and the products of their processing”. Analytical Biochemistry, 175, 227–230.
[27] Adebiyi, A. P., and Aluko, R. E. 2011. ‘Functional properties of protein fractions obtained from commercial yellow field pea (Pisum sativum L.) seed protein isolate’. Food Chemistry, 128 (4): 902-908.
[28] Maninder, K., Kawaljit, S. S and Narpinder, S (2007) Comparative study of functional, thermal and pasting properties of flours from different field pea and pigeon pea cultivars. Food Chem 104: 259–267.
[29] Takashi S. and Seib R. A. (1988). “Paste and gel properties of prime corn and wheat starches with and without native lipids”. Cereal Chemistry. 65: 474.
[30] Antakli, S., Sarkees, N. and Sarraf, T. (2015). Determination of vitamins on c18 column with particle size 3 µm in some manufactured food products by HPLC with UV-DAD/FLD detection. International Journal of Pharmacy and Pharmaceutical Sciences Vol 7, Issue 6.
[31] Adedeye, A. and Adewoke, K. (1992). Chemical Composition and Fatty acid Profiles of Cereals in Nigeria Food Chem., 44: 41-44.
[32] Girgih, A., Udenigwe, C., Li, H., Adebiyi, A., and Aluko, R. (2011). “Kinetics of enzyme inhibition and antihypertensive effects of hemp seed (Cannabis sativa L.) protein hydrolysates”. Journal of the American Oil Chemists Society, 88: 1767–1774.
[33] Benzie, I. F. F and Strain, J. J. (1999). “Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay”. Analytical Biochemistry, 239: 70-76.
[34] Xie, Z., Huang, J., Xu, X. and Jin, Z. (2008). Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate. Food Chemistry, 111: 370–376.
[35] Hoff, J., and Singleton, K. (1977). “A method for determination of tannins in foods by means of immobilized protein”. Journal of Food Science, 42 (6), 1566–1569.
[36] Nabavi, S. F., Nabavi, S. M., Ebrahimzadeh, M. A., Eslami, B., and Jafari, N. (2013). In vitro antioxidant and antihemolytic activities of hydroalcoholic extracts of Allium scabriscapum Boiss. & Ky. aerial parts and bulbs. International J. of Food Properties, 16 (4), 713–722.
[37] Siswoyo, T. A., Mardiana, E., Lee, K. O and Hoshokawa, K (2011). Isolation and characterization of antioxidant protein fractions from melinjo (Gnetum gnemon) seeds. J. Agric. Food Chem., 59: 5648-5656.
[38] Planesso, D., Neto, J. R., Da Silva, L. P., Goulart, F. R and Adorian, T. J (2015). Determination of Tryptophan requirements for juvenile silver catfish (Rhamdia quelen) and its effects on growth performance, plasma and hepatic metabolites and digestive enzymes activity. Anim. Feed Sci. Technol., 210: 172-183.
[39] Alsmeyer, R. H., Cunninghan, A. E and Happich, M. L (1974). Equations predict PER from amino acid analysis. Food Technology, 28: 34-40.
[40] Steinke, F. H., Presher, E. E and Hopkins, D. T (1980). Nutritional evaluation (PER) of isolated soy bean protein and combination of food proteins. Journal of Food Science, 45: 323-327.
[41] Oser, B. L (1959). An Integrated essential amino acid profile of the brain and eye of African giant punch kratd (Crieetomys gambianus). Agric Bist. J. North Am, 2: 368-375.
[42] Olaniran, A. F., Okonkwo, C. E., Owolabi, A. O, Osemwegie, O. O, and Badejo, T. E (2020). Proximate composition and physicochemical properties of formulated cassava, cowpea and potato flour blends. IOP Conf. Ser.: Earth Environ. Sci. 445 012042.
[43] Awolu, O. O and Osigwe, M. A (2019) Nutritional and Antioxidant Potential of Rice Flour Enriched with Kersting’s Groundnut (Kerstingiella geocarpa) and Lemon Pomace. International Journal of Food Studies. 8: 30–40.
[44] Bello, F. A., Oyeniyi, A. O. and George, I. O. (2019a) Chemical and Functional Properties of Wheat, Pigeon Pea and Plantain Composite Flour. European Journal of Food Science and Technology. 7 (4): 1-8.
[45] FAO/WHO (1998) Carbohydrates in human nutrition: report of a Joint FAO/WHO Expert Consultation, 14–18 April 1997, Rome. FAO Food and Nutrition Paper No. 66. Rome.
[46] Adejumo P. O., Adejumo A. O., Edebiri O. E and Olukoya F. O (2020). Effect of unripe banana and pigeon pea flour on the chemical, anti-nutritional and sensory properties of whole wheat-based cookies. GSC Advanced Research and Reviews, 04 (01), 017–023.
[47] Onwurafor, E. U., Uzodinma, E. O., Obeta, N. A and Akubueze, V. O (2020) Development and Quality Evaluation of Noodles from Wheat Flour Substituted with Maize and Mungbean Malt Flour. Pak. J. Nutr., 19 (7): 337-343.
[48] Hassan, L. G., Umar, K. J., and Umar, Z. (2004). Antinutritive factors in tribulus terrestris (linn.) leaves and predicted calcium and zinc bioavailability. Journal Tropical Bioscience, 7, 33–36.
[49] Ghavidel, R. A and Prakash, J (2007). The impact of germination and dehulling on nutrients, antinutrients, in vitro iron and calcium bioavailability and in vitro starch and protein digestibility of some legume seeds. Food Sci. and Technol. 40: 1292–1299.
[50] Achy, J. Y., Koffi, P. K. B., Ekissi, G. S. E., Konan, H. K and Kouame, L. P. (2016). Assessment of Physico-chemical Properties and Anti-nutritional factors of flour from Yam (Dioscorea bulbifera) Bulbils in Southeast Cote D’ivore. Int. J. Adv. Res. 4 (12): 871-88.
[51] Ajayi F. F., Kusimo O. O, Henshaw F. O and Ogori A. F (2017). “Proximate Composition and Functional properties of Rice (Oryza Sativa L.) Germinated-Cowpea (Vigna Unguiculata L.) flour blends”. Innovative Techniques in Agriculture 1.1: 32-40.
[52] Oppong D, Eric A, Samuel O. K, Eric B, and Patrick S (2015). Proximate Composition & Some Functional Properties of Soft Wheat Flour. Int’l J. of Innovative Research in Sci, Engineering and Technol. 4 (2): 753–758. DOI: 10.15680/IJIRSET.2015.0402097.
[53] Tortoe, C, Dowuona, S, Akonor, P. T and Dziedzoave1, N. T (2017). Examining the physicochemical, functional and rheological properties in flours of farmers’ 7 key yam (Dioscorea spp.) varieties in Ghana to enhance yam production. Cogent Food & Agriculture. 3: 1371564.
[54] Adamu, O. A and Akhere, E. P (2020). Proximate Composition, Functionality, and Pasting Properties of Orange Flesh Sweet Potato and Red Bambara Groundnut Flour Blends for Snacks Formulation. Asian Food Science Journal, 17 (1): 38-47.
[55] Meka, E., Igbabul, B. D., and Ikya, J (2019). Chemical and Functional Properties of Composite Flours Made from Yellow Maize, Soybeans, and Jackfruit Seed. International Journal of Research and Innovation in Applied Science (IJRIAS) | 4 (11): 57-63.
[56] Adegunwa, M. O., Adelekan, E. O., Adebowale, A. A., Bakare, H. A and Alamu, E. O (2017). Evaluation of nutritional and functional properties of plantain (Musa paradisiaca L.) and tigernut (Cyperus esculentus L.) flour blends for food formulations, Cogent Chemistry 3: 1383707.
[57] Rashid, S., Rakha, A., Anjum, F. M., Ahmed, W., and Sohail, M. (2015). Effects of extrusion cooking on the dietary fibre content and water solubility index of wheat bran extrudates. Internastional journal of Food Science and Technology, 50 (7), 1533-1537.
[58] Bello F. A, Edeke J. E, Sodipo M. A (2019b) Evaluation of Chemical, Functional and Sensory Properties of Flour Blends from Sorghum, African Yam Bean and Soybean for Use as Complementary Feeding. International Journal of Food Science and Biotechnology 4 (3): 74-81.
[59] Ocloo, F. C. K, Bansa D, Boatin, R, Adom, T, Agbemavor, W. S (2010). Physicohemical, functional and pasting characteristics of flour produced from Jackfruits (Artocarpus heterophyllus) seeds. Agriculture and Biology journal of North America. ISSN print: 2151-7517.
[60] Ayo, J. A and Gidado, F. E. (2017) Physicochemical, phytochemical and sensory evaluation of acha-carrot flours blend biscuit current. Journal of Applied Science and Technology known as British Journal of Applied Science & Technology. 25 (5): 1-15.
[61] Ayo, J. A and Okoye. E. (2020). Nutrient Composition and Functional Properties of Fonio (Digetaria exilis) and Amaranth (Amaranthus cruentus) Flour Blends. Asian Food Science Journal. 16 (3): 53-62.
[62] Sodipo, M. A., Lawal, O. M., Alabi, O. E., Solomon, O. O., Oluwamukomi, M. O and Oluwalana, I. B (2018). Physicochemical Properties and Amino Acid Profile of Extruded Products from Pearl Millet and Germinated Pigeon Pea. Annals. Food Science and Technology. 19 (2): 183-190.
[63] Siddiqua, A., Ali, M. S. and Ahmed, S (2019). Functional properties of germinated and non-germinated cereals: A comparative study. Bangladesh J. Sci. Ind. Res. 54 (4): 383-390.
[64] Egbujie, A. E. and Okoye, J. I. (2019) Quality Characteristics of Complementary Foods Formulated From Sorghum, African Yam Bean and Crayfish Flours. Science World Journal. 14 (2): 16-22.
[65] Babarinde, G. O, Adeyanju., J. A, Ogunleye, K. Y, Adegbola, G. M, Ebun, A. A, and Wadel, D.(2020) Nutritional composition of gluten-free flour from blend of fonio (Digitaria iburua) and pigeon pea (Cajanus cajan) and its suitability for breakfast food. J Food Sci Technol https://doi.org/10.1007/s13197-020-04393-7.
[66] Eze C. R., Okafor G. I., Omah E. C. and Azuka, C. E. (2020) Micronutrients, antinutrients composition and sensory properties of extruded snacks made from sorghum and charamenya flour blends. African Journal of Food Science. 14 (1): 25-31.
[67] Okaka, J. C., Akobundu, E. N. T. and Okaka, A. N. C. (2006). Food a nd Human Nutrition: An Integrated Approach, 2nd ed. Ocjanc o Academic Publishers, Enugu, Nigeria. Pp. 102-144.
[68] Okwu, D. E. (2004). Phytochemicals and vitamin content of indigenous spices of South-Eastern Nigeria. Journal of Sustainable Agriculture and Environmental Sciences; 6 (1): 30-37.
[69] Onimawo IA, and Asugo, S (2008) Effects of germination on the nutrient content and functional properties of pigeon pea flour. J Food Sci Technol 41: 170–174.
[70] Theodoratou, E., Farrington, S. M., Tenesa, A., McNeil, G., Cetnarskyj, R., Barnetson, R. A., Porteous, M. E., Dunlop, M. G and Campbell, H (2008). Dietary vitamin B6 intake and the risk of colorectal cancer. Cancer Epidemiol. Biomarkers Prev., 17, 171-182.
[71] Oyegoke, T. G., Adedayo E. O., Fasuyi F. O., and Oyegoke D. A (2020). Vitamin and Mineral Composition of Complementary Food Formulated from Yellow Maize, Soybean, Millet and Carrot Composite Flours. International Journal of Science and Research (IJSR), 9 (2): 450–456.
[72] Oyarekua, M. A., 2010. Sensory evaluation, nutritional quality and antinutritional factors of traditionally co-fermented cereals/cowpea mixtures as infant complementary food. Agric. Biol. J. N. Am., 1: 950-956.
[73] Mahloko, L. M; Silungwe H; Mashau, M. E and Kgatla, T. E (2019). Bioactive compounds, antioxidant activity and physical characteristics of wheat-prickly pear and banana biscuits. Heliyon 5 e02479.
[74] Bouhlal O, Taghouti M, Benbrahim N, Benali A, Visioni A and Benba J (2019). Wheat-lentil fortified flours: health benefits, physicochemical, nutritional and technological properties. J. Mater. Environ. Sci. 10 (11): 1098-1106.
[75] He, R., Girgih, A. T., Malomo, S. A., Ju, X and Aluko, R. E (2013) Antioxidant activities of enzymatic rapeseed protein hydrolysates and the membrane ultrafiltration fractions. Journal of functional foods. 5 (1): 219-227.
[76] Kaur, M., Asthiri, B and Mahajan, G (2017). Variation in Antioxidants, Bioactive Compounds and Antioxidant Capacity in Germinated and Ungerminated Grains of Ten Rice Cultivars. Rice Science, 24 (6): 349í359.
[77] Zhang, Y.; Lee, E. T.; Devereux, R. B.; Yeh, J.; Best, L. G.; Fabsitz, R. R. and Howard, B. V (2006). Prehypertension, diabetes, and cardiovascular disease risk in a population-based sample: The strong heart study. Hypertens, 47: 410–414.
[78] Hsu, A. L., Murphy, C. T., Kenyon, C (2003). Regulating of aging and age-related disease by DAF-16 and heat-shock factor. Science 300, 1142-1145.
[79] Dorman, H. J. D., Peltoketo, A., Hiltunen, R., Tikkanen, M. J. (2003). Characterization of the antioxidant properties of deodorised aqueous extracts from selected Lamiaceae herbs. Food Chemistry, 83 (2), 255–262.
[80] FAO/WHO. (1990). Protein Quality Evaluation. Report of joint FAO/WHO Expert Consultation. Held in Bethesda, USA, 4-8 December, 1989. FAO, Rome, Italy.
[81] Malomo, A. A and Abiose, S. H (2019). Protein quality and functional properties of masa produced from maize, acha and soybean. Food Research 3 (5): 556-563.
[82] Arogba S. S, Akpala S. N, Amlabu E and Amodu L (2020) Sumain nutritional supplement: Formulation, physico-chemical and nutritional assessment of its flour. GSC Biological and Pharmaceutical Sciences. 10 (03), 137–149.
[83] Fasuan T. O, Asadu K. C, Anyiam C. C, Ojokoh L. O, Olagunju T. M, Chima J. U and Okpara K. O (2021a) Bioactive and nutritional characterization of modeled and optimized consumer-ready flakes from pseudocereal (Amaranthus viridis), high-protein soymeal and modified corn starch. Food Production, Processing and Nutrition 3: 12 https://doi.org/10.1186/s43014-021-00057-x.
[84] Okpala, L. C., Egbadon, L and Okoye, S. (2016). Physicochemical and Protein Quality of Noodles made with Wheat and Okara flour blends. Pakistan journal of Nutrition 15 (9): 829-836.
[85] Fang, Y., Xingjian, H., Conglan, Z. C., Mei, Z., Chao, H. and Hao, Y. (2018). Amino acid composition and nutritional value evaluation of Chinese Chestnut (Castanea mollissima Blume) and its protein subunit. Royal Society of Chemistry, 8, 2653–2659.
[86] Fasuan T. O, Asadu K. C, Ojokoh L. O, Anyiam C. C, Olagunju T. M, Okpara K. O and Chima J. U (2021b) Ready-To-Eat Flakes from Acha, Partially Defatted Sesame Meal and Modified Corn Starch: Modeling, Optimization and Characterization. Food Sc and Nutri. Tech. 6 (1): 1-12.
[87] Olagunju, A. I., Ekeogu, P. C and Bamisi, O. C (2020). Partial substitution of whole wheat with acha and pigeon pea flours influences rheological properties of composite flours and quality of bread. https://www.emerald.com/insight/0007-070X.htm.
[88] Friedman, M (1996). Nutritional value of proteins from different food sources. A review. J. Agric. Fd. Chem., 446-29.
Cite This Article
  • APA Style

    Judith Uchenna Chima, Folusho Morenike David-Abraham, Laura Chioma Okpala. (2022). Activation of Cotyledon as Nutrients and Antioxidant Enhancer in Oryza sativa, Dioscorea bulbifera and Phaseolus vulgaris for Food Formulation. Science Development, 3(1), 31-45. https://doi.org/10.11648/j.scidev.20220301.15

    Copy | Download

    ACS Style

    Judith Uchenna Chima; Folusho Morenike David-Abraham; Laura Chioma Okpala. Activation of Cotyledon as Nutrients and Antioxidant Enhancer in Oryza sativa, Dioscorea bulbifera and Phaseolus vulgaris for Food Formulation. Sci. Dev. 2022, 3(1), 31-45. doi: 10.11648/j.scidev.20220301.15

    Copy | Download

    AMA Style

    Judith Uchenna Chima, Folusho Morenike David-Abraham, Laura Chioma Okpala. Activation of Cotyledon as Nutrients and Antioxidant Enhancer in Oryza sativa, Dioscorea bulbifera and Phaseolus vulgaris for Food Formulation. Sci Dev. 2022;3(1):31-45. doi: 10.11648/j.scidev.20220301.15

    Copy | Download

  • @article{10.11648/j.scidev.20220301.15,
      author = {Judith Uchenna Chima and Folusho Morenike David-Abraham and Laura Chioma Okpala},
      title = {Activation of Cotyledon as Nutrients and Antioxidant Enhancer in Oryza sativa, Dioscorea bulbifera and Phaseolus vulgaris for Food Formulation},
      journal = {Science Development},
      volume = {3},
      number = {1},
      pages = {31-45},
      doi = {10.11648/j.scidev.20220301.15},
      url = {https://doi.org/10.11648/j.scidev.20220301.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.scidev.20220301.15},
      abstract = {The impact of cotyledon activation on brown rice Rice (Oryza sativa), Aerial yam (Dioscorea bulbifera) and black turtle bean (Phaseolus vulgaris) was studied. Composite flours were prepared using cotyledon activated brown rice (GBR), aerial yam (AYF) and black turtle bean (GTB). The ratio of GBR: AYF: GTB used in the flour formulations were: 100GBR: 0AYF: 0GTB; 0GBR: 100AYF: 0GTB; 0GBR: 0AYF: 100GTB; 33.33GBR: 33.33AYF: 33.33GTB; 66.67GBR: 16.67AYF: 16.67GTB, 16.67GBR: 66.67AYF: 16.67GTB and 16.67GBR: 16.67AYF: 66.67GTB. Proximate composition, functional properties, antinutrients, minerals, vitamins, amino acid profile and antioxidant activity of the composite flours were determined using standard procedures. Protein efficiency ratio, essential amino acid index and biological value were calculated from the amino acid profile. Results showed ash content (2.72-3.20%), fibre (2.12-2.42%), protein (13.23-39.31%), tannin (0.973-1.120mg/100g), saponin (1.95-2.44 mg/g), oxalate (2.50-3.38mg/g), water absorption capacity (107.71–190.23%), oil absorption capacity (145.30-296.70%), water solubility index (16.05-17.78%), swelling capacity (230.10-250.50%), calcium (36.64-40.62mg/100g), phosphorus (84.84-88.93mg/100g), manganese (0.46-0.57mg/100g), magnesium (1741.20-1823.20mg/100g), vitamin B1 (2.75-4.06mg/100g, B2 (2.01-2.78 mg/100g, B3 (2.20-4.83mg/100g), B6 (2.63-3.98mg/100g) B12 (1.59-5.95mg/100g), essential amino acid index (70-99%), protein efficiency ratio (1.95–3.79), and biological value (70-96%). Nutrients and antioxidant properties were improved through the cotyledon activation process, level of antinutrients were safe and below toxicity level. The composite flour is invaluable as functional ingredient in food formulation.},
     year = {2022}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Activation of Cotyledon as Nutrients and Antioxidant Enhancer in Oryza sativa, Dioscorea bulbifera and Phaseolus vulgaris for Food Formulation
    AU  - Judith Uchenna Chima
    AU  - Folusho Morenike David-Abraham
    AU  - Laura Chioma Okpala
    Y1  - 2022/02/28
    PY  - 2022
    N1  - https://doi.org/10.11648/j.scidev.20220301.15
    DO  - 10.11648/j.scidev.20220301.15
    T2  - Science Development
    JF  - Science Development
    JO  - Science Development
    SP  - 31
    EP  - 45
    PB  - Science Publishing Group
    SN  - 2994-7154
    UR  - https://doi.org/10.11648/j.scidev.20220301.15
    AB  - The impact of cotyledon activation on brown rice Rice (Oryza sativa), Aerial yam (Dioscorea bulbifera) and black turtle bean (Phaseolus vulgaris) was studied. Composite flours were prepared using cotyledon activated brown rice (GBR), aerial yam (AYF) and black turtle bean (GTB). The ratio of GBR: AYF: GTB used in the flour formulations were: 100GBR: 0AYF: 0GTB; 0GBR: 100AYF: 0GTB; 0GBR: 0AYF: 100GTB; 33.33GBR: 33.33AYF: 33.33GTB; 66.67GBR: 16.67AYF: 16.67GTB, 16.67GBR: 66.67AYF: 16.67GTB and 16.67GBR: 16.67AYF: 66.67GTB. Proximate composition, functional properties, antinutrients, minerals, vitamins, amino acid profile and antioxidant activity of the composite flours were determined using standard procedures. Protein efficiency ratio, essential amino acid index and biological value were calculated from the amino acid profile. Results showed ash content (2.72-3.20%), fibre (2.12-2.42%), protein (13.23-39.31%), tannin (0.973-1.120mg/100g), saponin (1.95-2.44 mg/g), oxalate (2.50-3.38mg/g), water absorption capacity (107.71–190.23%), oil absorption capacity (145.30-296.70%), water solubility index (16.05-17.78%), swelling capacity (230.10-250.50%), calcium (36.64-40.62mg/100g), phosphorus (84.84-88.93mg/100g), manganese (0.46-0.57mg/100g), magnesium (1741.20-1823.20mg/100g), vitamin B1 (2.75-4.06mg/100g, B2 (2.01-2.78 mg/100g, B3 (2.20-4.83mg/100g), B6 (2.63-3.98mg/100g) B12 (1.59-5.95mg/100g), essential amino acid index (70-99%), protein efficiency ratio (1.95–3.79), and biological value (70-96%). Nutrients and antioxidant properties were improved through the cotyledon activation process, level of antinutrients were safe and below toxicity level. The composite flour is invaluable as functional ingredient in food formulation.
    VL  - 3
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Department of Food Technology, Akanu Ibiam Federal Polytechnic Unwana, Unwana, Nigeria

  • Department of Food Science and Technology, Ebonyi State University, Abakaliki, Nigeria

  • Department of Food Science and Technology, Ebonyi State University, Abakaliki, Nigeria

  • Sections