Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases

Maina Susan Muthoni

doi:doi:10.11648/j.jher.20210703.14

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Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases

Maina Susan Muthoni

Published in Journal of Health and Environmental Research (Volume 7, Issue 3)

Received: 18 June 2021 Accepted: 6 July 2021 Published: 24 July 2021

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Abstract

The key to success of healthcare quality is the control of hospital environment. Challenge of infection prevention and epidemiology practice continues to be an increasing emergence and spread of pathogenic bacteria is of great concern. The study is about the prevalence and isolation of bacteria from hospital surfaces environment in Kenya. About 246 samples of the two hospitals was obtained using sterile cotton swabs from random sampling of hospital different surfaces, drainages, hands of healthcare givers and hospital waste dump site among others. The samples were aseptically collected, transported and processed following standard procedures. Bacteria were isolated and identified using various biochemical tests and confirmed using API 20E. Door handle surfaces had the highest percentage of isolated bacteria (13%) while the least was cupboard surfaces (3%) in both hospitals. Kenyatta National Hospital (KNH) had the most isolated bacteria as compared to Kikuyu Mission Hospital (KMH) in most swabbed surfaces; the difference however was not significant. Various bacteria were isolated where the most abundant bacteria in both hospitals was Providencia species, while the least was Enterobacter species. There is therefore, high bacteria contamination of objects of hospitals frequently touched sites and surfaces act as a source of infectious diseases as they harbor potential pathogens.

Published in	Journal of Health and Environmental Research (Volume 7, Issue 3)
DOI	10.11648/j.jher.20210703.14
Page(s)	139-144
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Surfaces, Hospital, Contamination, Bacteria

References

[1]	Madhukumar, S. & Ramesh, G. (2012). Study about Awareness and Practices about Health Care Wastes Management among Hospital Staff in a Medical College Hospital, Bangalore. International Journal of Basic Medical Science, 3 (1), 7-11.
[2]	Nkonge, N. A, Mayabi, O. A, & Kithinji, J. (2012). Knowledge, attitude and practice of health-care waste management and associated health risks in the two teaching and referral hospitals in Kenya. Journal Community Health: 37 (60), 1172-7.
[3]	Bakalli M. E. L, Hamid K, Kari K. E, Zouhdi M, Mzibri M. E. L (2015). Characterization of bacterial strains and their resistance status in hospital environment, Journal of Tropical diseases 4: 180. DOI 10.4172/2329-891X.1000180.
[4]	Nwachukwu, N. C., Orji, F. A., & Ugbogu, O. C. (2013). Health care waste management–public health benefits, and the need for effective environmental regulatory surveillance in federal Republic of Nigeria. Current topics in public health, 2, 149-178.
[5]	Otter, J., Yezli, S. and French, G. (2011). The role played by contaminated surfaces in the transmission of nosocomial pathogens. Journal of Infections Control and Hospital Epidemiology, 32 (7): 687-699.
[6]	Samuel, S. O., Kayode, O. O., Musa, O. I., Nwigwe, G. C., Aboderin, A. O., Salami, T. A. T. and Taiwo, S. S. (2010). Nosocomial infections and the challenges of control in developing countries. African Journal of Clinical and Experimental Microbiology, 11 (2): 102–110.
[7]	Yagoub, S. O, Amani E. & Agbah, L. (2010). Isolation of potential pathogenic bacteria from the air of hospital. Journal of Applied Science, 10, 1011-1014.
[8]	Wen, Y., Yiting, W., Lu, L., & Jin, H. (2019). Biofilms, the microbial protective clothing in extreme environments. International Journal of Molecular Science. 20, 3423-3428.
[9]	Moges, F, Mengistu E, Yeshambel B and Walelegn W, (2014). Isolation and characterization of multiple drug resistance bacterial pathogens from waste water in hospital and non-hospital environments, Northwest Ethiopia BMC Research Notes 7: 215.
[10]	Rezai, M. S. Salehifar, E, Rafier, A, Langaee, T., Rafito, M Shafahi, K, & Eslami, G, (2015) Characteristics of multidrug resistant extended- spectrum beta lactamase- producing E. coli among uropathogens of pediatrics in North of Iran. Biomed. Research International, 1-7.
[11]	Olalekan, A. W., Asekun-Olarinmoye, E. O., Bamidele, J. O., Abodunrin, O. L. and Olowu, A. (2011). A comparative study of awareness and attitude to nosocomial infections among levels of health care workers in Southwestern Nigeria. Continental Journal of Tropical Medicine, 5 (2): 5-10.
[12]	Odeyemi, A. T (2012). Antibiogram status of bacterial Isolate from air around dumpsite of Ekiti State Destitute Centre at IIokun, Ado-Ekiti, Nigeria. Journal of Microbiology Research. 2, 12-18.
[13]	Jayanthi, A. (2014). Most common healthcare-associated infections: 25 bacteria, viruses causing HAIs, Becker's hospital review.
[14]	Nworie, A., Ayeni, J. A., Eze, U. A. and Azi, S. O. (2012). Bacterial contamination of door handles/knobs in selected public conveniences in Abuja Metropolis, Nigeria: A public health threat. Continental Journal of Medical Research, 6 (1): 7-11.
[15]	Nejad, S. B, Syed, S. B, Ellis, B, Pittet, D. (2011). Health associated infection in Africa: a systematic review. Bulletin of the World Health Organization; 89, 757-65.
[16]	White, K. M., Jimmieson, N. L., Obst, P. L, Graves, N., Barnet, A., Cockshaw, W., Paterson, D. (2015). Using a theory of planned behavior framework to explore hand hygiene beliefs at the 5 critical moments among Australian hospital-based nurses. Biomed Centre Health Services Research, 15, 15-59. doi: 10.1186/s12913-015-0718-2.
[17]	Frost M, and Sullivan K (2010). Hospital acquired infections – Trends across Europe, M56E-54.
[18]	Allegranzi, B., and Pittet, D. (2009). Role of Hand Hygiene in Healthcare-Associated Infection Prevention. The Journal of Hospital Infection, 73 (4): 305–315.
[19]	Srinivas, P., Reddy, S. R., (2012). Screening for antibacterial principle and activity of Aerva javanica Juss. Ex Schult. Asian Pacific. Journal. Tropical. Biomedical. 2 (Supplement 2): 838-845.
[20]	Rajat, R., Ninama, G., Mistry, K., Parmar, R., Patel, K., and Vegad, M. (2012). Antibiotic resistance pattern in Pseudomonas species isolated at a tertiary care hospital, Ahmadabad, National Journal of Medicine. 2 (2): 156-159.
[21]	Neidig, A, Yeung, A, & Joerg, O. (2013). Type A is involved in virulence, antimicrobial resistance and biofilm formation in Pseudomonas aeruginosa. Bio MedCentral Microbiology, 77, 1-23.
[22]	Udeze, A. O., Adeyemi, A. T., Adeniji, F. O., Nwanze, J. C., Onoh, C., Okerentubga, P. O. and Okonko, I. O. (2012). Plasmid mediated ampicillin resistant bacteria isolates from University of Ilorin Health Centre. New York Science Journal, 5 (4): 56–63.
[23]	Pal, R. B., Rodrigues, M. and Datta, S. (2010). Role of Pseudomonas in nosocomial infections and biological characterization of local strains. Journal of Bioscience Technology, 1 (4): 170-179.
[24]	Mlynarczyk, A, Mlynarczyk, G, Pupek, J, Bilewska, A, Kawecki, D, Luczak, M, Godowsky, J, Durlik, M, Paczek, L, Chmura, A, Rowinnski, W. (2007). Serratia marscens isolated in 2005 from clinical specimens from patients with diminished immunity. Transplantation Proceedings 39 (9): 2879-2882.
[25]	Matinyi, S., Enoch, M., Akia, D. (2019). Contamination of microbial pathogens and their antimicrobial pattern in operating theatres of peri-urban eastern Uganda; a cross sectional study. BMC Infect Dis 18, 460.
[26]	Anitha, J, Jayraaj I. A. (2012). Isolation and identification of bacteria from bio-hospital waste (BMW). International Journal of Pharmacy and Pharmaceutical Sciences, 4 (5): 1-3.
[27]	Liberto, M. C, Matera R, Piccis, T, Lo Russo E, Colosima and E. Foca (2009). Six cases of sepsis caused by Pantoea agglomelans in a teaching hospital. New Microbial 32; 119-23.

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APA Style

Maina Susan Muthoni. (2021). Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases. Journal of Health and Environmental Research, 7(3), 139-144. https://doi.org/10.11648/j.jher.20210703.14

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ACS Style

Maina Susan Muthoni. Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases. J. Health Environ. Res. 2021, 7(3), 139-144. doi: 10.11648/j.jher.20210703.14

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AMA Style

Maina Susan Muthoni. Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases. J Health Environ Res. 2021;7(3):139-144. doi: 10.11648/j.jher.20210703.14

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@article{10.11648/j.jher.20210703.14,
  author = {Maina Susan Muthoni},
  title = {Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases},
  journal = {Journal of Health and Environmental Research},
  volume = {7},
  number = {3},
  pages = {139-144},
  doi = {10.11648/j.jher.20210703.14},
  url = {https://doi.org/10.11648/j.jher.20210703.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20210703.14},
  abstract = {The key to success of healthcare quality is the control of hospital environment. Challenge of infection prevention and epidemiology practice continues to be an increasing emergence and spread of pathogenic bacteria is of great concern. The study is about the prevalence and isolation of bacteria from hospital surfaces environment in Kenya. About 246 samples of the two hospitals was obtained using sterile cotton swabs from random sampling of hospital different surfaces, drainages, hands of healthcare givers and hospital waste dump site among others. The samples were aseptically collected, transported and processed following standard procedures. Bacteria were isolated and identified using various biochemical tests and confirmed using API 20E. Door handle surfaces had the highest percentage of isolated bacteria (13%) while the least was cupboard surfaces (3%) in both hospitals. Kenyatta National Hospital (KNH) had the most isolated bacteria as compared to Kikuyu Mission Hospital (KMH) in most swabbed surfaces; the difference however was not significant. Various bacteria were isolated where the most abundant bacteria in both hospitals was Providencia species, while the least was Enterobacter species. There is therefore, high bacteria contamination of objects of hospitals frequently touched sites and surfaces act as a source of infectious diseases as they harbor potential pathogens.},
 year = {2021}
}

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TY - JOUR
T1 - Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases
AU - Maina Susan Muthoni
Y1 - 2021/07/24
PY - 2021
N1 - https://doi.org/10.11648/j.jher.20210703.14
DO - 10.11648/j.jher.20210703.14
T2 - Journal of Health and Environmental Research
JF - Journal of Health and Environmental Research
JO - Journal of Health and Environmental Research
SP - 139
EP - 144
PB - Science Publishing Group
SN - 2472-3592
UR - https://doi.org/10.11648/j.jher.20210703.14
AB - The key to success of healthcare quality is the control of hospital environment. Challenge of infection prevention and epidemiology practice continues to be an increasing emergence and spread of pathogenic bacteria is of great concern. The study is about the prevalence and isolation of bacteria from hospital surfaces environment in Kenya. About 246 samples of the two hospitals was obtained using sterile cotton swabs from random sampling of hospital different surfaces, drainages, hands of healthcare givers and hospital waste dump site among others. The samples were aseptically collected, transported and processed following standard procedures. Bacteria were isolated and identified using various biochemical tests and confirmed using API 20E. Door handle surfaces had the highest percentage of isolated bacteria (13%) while the least was cupboard surfaces (3%) in both hospitals. Kenyatta National Hospital (KNH) had the most isolated bacteria as compared to Kikuyu Mission Hospital (KMH) in most swabbed surfaces; the difference however was not significant. Various bacteria were isolated where the most abundant bacteria in both hospitals was Providencia species, while the least was Enterobacter species. There is therefore, high bacteria contamination of objects of hospitals frequently touched sites and surfaces act as a source of infectious diseases as they harbor potential pathogens.
VL - 7
IS - 3
ER -

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Author Information

Maina Susan Muthoni

Department of Medical Microbiology, School of Biomedical Sciences, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi, Kenya

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APA Style

Maina Susan Muthoni. (2021). Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases. Journal of Health and Environmental Research, 7(3), 139-144. https://doi.org/10.11648/j.jher.20210703.14

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ACS Style

Maina Susan Muthoni. Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases. J. Health Environ. Res. 2021, 7(3), 139-144. doi: 10.11648/j.jher.20210703.14

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AMA Style

Maina Susan Muthoni. Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases. J Health Environ Res. 2021;7(3):139-144. doi: 10.11648/j.jher.20210703.14

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@article{10.11648/j.jher.20210703.14,
  author = {Maina Susan Muthoni},
  title = {Hospitals Surfaces and Sites as a Reservoir for Pathogenic Bacteria That Play a Role in Transmission of Infectious Diseases},
  journal = {Journal of Health and Environmental Research},
  volume = {7},
  number = {3},
  pages = {139-144},
  doi = {10.11648/j.jher.20210703.14},
  url = {https://doi.org/10.11648/j.jher.20210703.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20210703.14},
  abstract = {The key to success of healthcare quality is the control of hospital environment. Challenge of infection prevention and epidemiology practice continues to be an increasing emergence and spread of pathogenic bacteria is of great concern. The study is about the prevalence and isolation of bacteria from hospital surfaces environment in Kenya. About 246 samples of the two hospitals was obtained using sterile cotton swabs from random sampling of hospital different surfaces, drainages, hands of healthcare givers and hospital waste dump site among others. The samples were aseptically collected, transported and processed following standard procedures. Bacteria were isolated and identified using various biochemical tests and confirmed using API 20E. Door handle surfaces had the highest percentage of isolated bacteria (13%) while the least was cupboard surfaces (3%) in both hospitals. Kenyatta National Hospital (KNH) had the most isolated bacteria as compared to Kikuyu Mission Hospital (KMH) in most swabbed surfaces; the difference however was not significant. Various bacteria were isolated where the most abundant bacteria in both hospitals was Providencia species, while the least was Enterobacter species. There is therefore, high bacteria contamination of objects of hospitals frequently touched sites and surfaces act as a source of infectious diseases as they harbor potential pathogens.},
 year = {2021}
}

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