M. indica and B. dalzeilii are common medicinal plants grown naturally in most parts of tropical and subtropical regions of Northern Nigeria. They are used traditionally in the treatment of diarrhea in children especially at teething age. This study was conducted to detect, isolate and identify E. coli O157:H7 from diarrheal stools of children and evaluate antibacterial activities of M. indica and B. dalzielii extracts against the test bacteria. Total number of one hundred (100) samples were collected from Children Clinic of General Hospital Gusau, Zamfara State Nigeria using standard microbiological procedure. E. coli O157:H7 is an important pathogens of human, implicated as one of the important causative agents of diarrhea in children especially at teething ages. The test organism was detected, isolated and identified using Cultural Growth Characteristics, Gram reactions, Serology, Biochemical tests and Molecular screenings. The sample plants extracts were obtained using Soxhlex Apparatus with Methanol as solvent. Phytochemical screening of the plants extracts revealed the presence of Alkaloids, Flavoids, Tannins and Saponnins. Antibacterial activities of the aqueous methanolic extracts of the test plants against E. coli O157:H7 was evaluated using Sensitivity Tests, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal concentration (MBC). The most active phytochemical compounds was obtained by fractionation using Chromatography. Chemical structure of the most active phytochemical was elucidated using Nuclear Magnetic Resonance Spectroscopy. Confirmation of antibacterial activities of the test plants, lend credence to the traditional used of those plants in the treatment of diarrhea in children especially at teething ages. It is therefore recommended that, M. indica and B. dalzeilii can be further purified to yield templates for synthesis of Orthodox drugs for the treatment of diarrhea in children.
| Published in | International Journal of Microbiology and Biotechnology (Volume 8, Issue 2) |
| DOI | 10.11648/j.ijmb.20230802.11 |
| Page(s) | 30-36 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Molecular Detection, Antibacterial Activity, E. coli O157:H7, M. indica, B. dalzeilii, Phytochemical Screening, Diarrhea
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APA Style
Shinkafi Abubakar Liman, Yarima Mohammaed Bello, Deeni Yusuf Yahaya, Danjuma Lawal. (2023). Molecular Detection of E. coli O157:H7 Isolated from Infants Diarrheal Stools and Its Sensitivity to Mangifera indica (Mango) and Bosweilia dalzeilii (Hano) Extracts. International Journal of Microbiology and Biotechnology, 8(2), 30-36. https://doi.org/10.11648/j.ijmb.20230802.11
ACS Style
Shinkafi Abubakar Liman; Yarima Mohammaed Bello; Deeni Yusuf Yahaya; Danjuma Lawal. Molecular Detection of E. coli O157:H7 Isolated from Infants Diarrheal Stools and Its Sensitivity to Mangifera indica (Mango) and Bosweilia dalzeilii (Hano) Extracts. Int. J. Microbiol. Biotechnol. 2023, 8(2), 30-36. doi: 10.11648/j.ijmb.20230802.11
AMA Style
Shinkafi Abubakar Liman, Yarima Mohammaed Bello, Deeni Yusuf Yahaya, Danjuma Lawal. Molecular Detection of E. coli O157:H7 Isolated from Infants Diarrheal Stools and Its Sensitivity to Mangifera indica (Mango) and Bosweilia dalzeilii (Hano) Extracts. Int J Microbiol Biotechnol. 2023;8(2):30-36. doi: 10.11648/j.ijmb.20230802.11
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Download@article{10.11648/j.ijmb.20230802.11,
author = {Shinkafi Abubakar Liman and Yarima Mohammaed Bello and Deeni Yusuf Yahaya and Danjuma Lawal},
title = {Molecular Detection of E. coli O157:H7 Isolated from Infants Diarrheal Stools and Its Sensitivity to Mangifera indica (Mango) and Bosweilia dalzeilii (Hano) Extracts},
journal = {International Journal of Microbiology and Biotechnology},
volume = {8},
number = {2},
pages = {30-36},
doi = {10.11648/j.ijmb.20230802.11},
url = {https://doi.org/10.11648/j.ijmb.20230802.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20230802.11},
abstract = {M. indica and B. dalzeilii are common medicinal plants grown naturally in most parts of tropical and subtropical regions of Northern Nigeria. They are used traditionally in the treatment of diarrhea in children especially at teething age. This study was conducted to detect, isolate and identify E. coli O157:H7 from diarrheal stools of children and evaluate antibacterial activities of M. indica and B. dalzielii extracts against the test bacteria. Total number of one hundred (100) samples were collected from Children Clinic of General Hospital Gusau, Zamfara State Nigeria using standard microbiological procedure. E. coli O157:H7 is an important pathogens of human, implicated as one of the important causative agents of diarrhea in children especially at teething ages. The test organism was detected, isolated and identified using Cultural Growth Characteristics, Gram reactions, Serology, Biochemical tests and Molecular screenings. The sample plants extracts were obtained using Soxhlex Apparatus with Methanol as solvent. Phytochemical screening of the plants extracts revealed the presence of Alkaloids, Flavoids, Tannins and Saponnins. Antibacterial activities of the aqueous methanolic extracts of the test plants against E. coli O157:H7 was evaluated using Sensitivity Tests, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal concentration (MBC). The most active phytochemical compounds was obtained by fractionation using Chromatography. Chemical structure of the most active phytochemical was elucidated using Nuclear Magnetic Resonance Spectroscopy. Confirmation of antibacterial activities of the test plants, lend credence to the traditional used of those plants in the treatment of diarrhea in children especially at teething ages. It is therefore recommended that, M. indica and B. dalzeilii can be further purified to yield templates for synthesis of Orthodox drugs for the treatment of diarrhea in children.},
year = {2023}
}
TY - JOUR T1 - Molecular Detection of E. coli O157:H7 Isolated from Infants Diarrheal Stools and Its Sensitivity to Mangifera indica (Mango) and Bosweilia dalzeilii (Hano) Extracts AU - Shinkafi Abubakar Liman AU - Yarima Mohammaed Bello AU - Deeni Yusuf Yahaya AU - Danjuma Lawal Y1 - 2023/05/22 PY - 2023 N1 - https://doi.org/10.11648/j.ijmb.20230802.11 DO - 10.11648/j.ijmb.20230802.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 30 EP - 36 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20230802.11 AB - M. indica and B. dalzeilii are common medicinal plants grown naturally in most parts of tropical and subtropical regions of Northern Nigeria. They are used traditionally in the treatment of diarrhea in children especially at teething age. This study was conducted to detect, isolate and identify E. coli O157:H7 from diarrheal stools of children and evaluate antibacterial activities of M. indica and B. dalzielii extracts against the test bacteria. Total number of one hundred (100) samples were collected from Children Clinic of General Hospital Gusau, Zamfara State Nigeria using standard microbiological procedure. E. coli O157:H7 is an important pathogens of human, implicated as one of the important causative agents of diarrhea in children especially at teething ages. The test organism was detected, isolated and identified using Cultural Growth Characteristics, Gram reactions, Serology, Biochemical tests and Molecular screenings. The sample plants extracts were obtained using Soxhlex Apparatus with Methanol as solvent. Phytochemical screening of the plants extracts revealed the presence of Alkaloids, Flavoids, Tannins and Saponnins. Antibacterial activities of the aqueous methanolic extracts of the test plants against E. coli O157:H7 was evaluated using Sensitivity Tests, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal concentration (MBC). The most active phytochemical compounds was obtained by fractionation using Chromatography. Chemical structure of the most active phytochemical was elucidated using Nuclear Magnetic Resonance Spectroscopy. Confirmation of antibacterial activities of the test plants, lend credence to the traditional used of those plants in the treatment of diarrhea in children especially at teething ages. It is therefore recommended that, M. indica and B. dalzeilii can be further purified to yield templates for synthesis of Orthodox drugs for the treatment of diarrhea in children. VL - 8 IS - 2 ER -
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