Potential Gene Flow Between <i>Gossypium hirsitum</i> and <i>Abelmoschus esculentus</i> Through Interspecific Hybridization

Molo Thierry; Likeng-Li-Ngue Benoit Constant; Ngalle Hermine Bille; Nyouma Achille; Mbo Nkoulou Luther Fort; Eya’a Ngombo Clement; Bell Joseph Martin

doi:doi:10.11648/j.ijgg.20231101.11

Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, Eya’a Ngombo Clement, Bell Joseph Martin

Published in International Journal of Genetics and Genomics (Volume 11, Issue 1)

Received: 2 January 2023 Accepted: 25 January 2023 Published: 24 February 2023

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Abstract

The introduction of transgenic varieties of cotton (Gossypium hirsitum) in Cameroon has posed numerous concerns. One of the most concern was the horizontal transfer of the Bt gene to related species such as Abelmoschus esculentus. The current study was conducted to assess the occurrence Bt gene transfer from Gossypium hirsitum to Abelmoschus esculentus. To achieve this, the stigma receptivity periods and the viability of pollen grains were evaluated. The fruit set rates of 675 controlled intra- and interspecific crosses were assessed. The results showed that stigma receptivity and pollen grain viability are optimal at anthesis in both species, with a longer duration over 24 hours in G. hirsitum. Intra-specific crosses within the two species have shown fruit set rates of nearly 60-80%. Interspecific crosses gave a fruit set rate close to 2%, especially when A. esculentus is used as the female parent, and zero results in reciprocal crosses. The finding of the study pointed out that the possibility of horizontal transfer of a transgene to cotton relatives. In future studies, it would be useful to monitor the fate of seeds from interspecific crosses between both species and assess the agro-morphological and molecular characteristics of these possible hybrids.

Published in	International Journal of Genetics and Genomics (Volume 11, Issue 1)
DOI	10.11648/j.ijgg.20231101.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Interspecific Hybridization, G. hirsitum, A. esculentus, Gene Flow, Trangenic Varieties

References

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[5]	Badiane, O., Ghura, D., Goreux, L and Masson, P. (2002). Cotton sector strategies in West and Central Africa. World Bank Policy Research Working 2867, 53-59.
[6]	MINMIDT. (2015). Forum national sur les Organismes Génétiquement Modifiés au Cameroun: Enjeux et défis des OGM pour un développement durable du Cameroun.
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[9]	Rache, C., Leidy, Y., Mora-Oberleander, J., and Chaparro-Giraldo, A. (2013). Study of gene flow from gm cotton (gossypium hirsutum) varieties in “el espinal”. Acta Biológica Colombiana, 18 (3), 489-498.
[10]	Montes, M., Coriton, O., Eber, F., Huteau, V., Lacape, J. M., Reinhardt, C., Marais, D., Hofs, J. L., Chèvre, A. M and Pannetier, C. (2017). Assessment of gene flow between Gossypium hirsutum and G. herbaceum: Evidence of unreduced gametes in the diploid progenitor. Gene/Genome/Genetics, 7, 2185-2193. doi.org/10.1534/g3.117.041509.
[11]	Athanasios, G. M., Chrysanthi, I. P., Dimitrios, N. V., Ioannis, N. X., Dimitrios, G. R. (2018). Hybridization between cotton and Malvaceae species as a tool for production of partial interspecific aneuploidy cotton plants. Euphytica, 214 (179), 1-13, doi.org/10.1007/s10681-018-2257-5.
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[14]	Amiteye, S., Amitaaba, T., Akama, C., Harry, M. A. (2019). Hybridization Studies of Okra (Abelmoschus spp. (L.) Moench) Accessions. International Journal of Biotech Trends and Technology, 9 (4), 42-47.
[15]	Avneesh Kumar Singh., Vyakaranahal, B., Deshpande, V., Basavaraj, and Patil, M. (2010). Studies on stigma receptivity, bud size and pollination frequency for hybrid seed production in bhendi (Abelmoschus esculentus (l.) Moench). Karnataka Journal of Agriculture Sciences., 23 (3), 426-430.
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Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, et al. (2023). Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. International Journal of Genetics and Genomics, 11(1), 1-5. https://doi.org/10.11648/j.ijgg.20231101.11

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ACS Style

Molo Thierry; Likeng-Li-Ngue Benoit Constant; Ngalle Hermine Bille; Nyouma Achille; Mbo Nkoulou Luther Fort, et al. Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. Int. J. Genet. Genomics 2023, 11(1), 1-5. doi: 10.11648/j.ijgg.20231101.11

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AMA Style

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, et al. Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. Int J Genet Genomics. 2023;11(1):1-5. doi: 10.11648/j.ijgg.20231101.11

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@article{10.11648/j.ijgg.20231101.11,
  author = {Molo Thierry and Likeng-Li-Ngue Benoit Constant and Ngalle Hermine Bille and Nyouma Achille and Mbo Nkoulou Luther Fort and Eya’a Ngombo Clement and Bell Joseph Martin},
  title = {Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization},
  journal = {International Journal of Genetics and Genomics},
  volume = {11},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ijgg.20231101.11},
  url = {https://doi.org/10.11648/j.ijgg.20231101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20231101.11},
  abstract = {The introduction of transgenic varieties of cotton (Gossypium hirsitum) in Cameroon has posed numerous concerns. One of the most concern was the horizontal transfer of the Bt gene to related species such as Abelmoschus esculentus. The current study was conducted to assess the occurrence Bt gene transfer from Gossypium hirsitum to Abelmoschus esculentus. To achieve this, the stigma receptivity periods and the viability of pollen grains were evaluated. The fruit set rates of 675 controlled intra- and interspecific crosses were assessed. The results showed that stigma receptivity and pollen grain viability are optimal at anthesis in both species, with a longer duration over 24 hours in G. hirsitum. Intra-specific crosses within the two species have shown fruit set rates of nearly 60-80%. Interspecific crosses gave a fruit set rate close to 2%, especially when A. esculentus is used as the female parent, and zero results in reciprocal crosses. The finding of the study pointed out that the possibility of horizontal transfer of a transgene to cotton relatives. In future studies, it would be useful to monitor the fate of seeds from interspecific crosses between both species and assess the agro-morphological and molecular characteristics of these possible hybrids.},
 year = {2023}
}

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TY - JOUR
T1 - Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization
AU - Molo Thierry
AU - Likeng-Li-Ngue Benoit Constant
AU - Ngalle Hermine Bille
AU - Nyouma Achille
AU - Mbo Nkoulou Luther Fort
AU - Eya’a Ngombo Clement
AU - Bell Joseph Martin
Y1 - 2023/02/24
PY - 2023
N1 - https://doi.org/10.11648/j.ijgg.20231101.11
DO - 10.11648/j.ijgg.20231101.11
T2 - International Journal of Genetics and Genomics
JF - International Journal of Genetics and Genomics
JO - International Journal of Genetics and Genomics
SP - 1
EP - 5
PB - Science Publishing Group
SN - 2376-7359
UR - https://doi.org/10.11648/j.ijgg.20231101.11
AB - The introduction of transgenic varieties of cotton (Gossypium hirsitum) in Cameroon has posed numerous concerns. One of the most concern was the horizontal transfer of the Bt gene to related species such as Abelmoschus esculentus. The current study was conducted to assess the occurrence Bt gene transfer from Gossypium hirsitum to Abelmoschus esculentus. To achieve this, the stigma receptivity periods and the viability of pollen grains were evaluated. The fruit set rates of 675 controlled intra- and interspecific crosses were assessed. The results showed that stigma receptivity and pollen grain viability are optimal at anthesis in both species, with a longer duration over 24 hours in G. hirsitum. Intra-specific crosses within the two species have shown fruit set rates of nearly 60-80%. Interspecific crosses gave a fruit set rate close to 2%, especially when A. esculentus is used as the female parent, and zero results in reciprocal crosses. The finding of the study pointed out that the possibility of horizontal transfer of a transgene to cotton relatives. In future studies, it would be useful to monitor the fate of seeds from interspecific crosses between both species and assess the agro-morphological and molecular characteristics of these possible hybrids.
VL - 11
IS - 1
ER -

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Author Information

Molo Thierry

Genetic and Plant Improvement Unit, Department of Plant Biology, Faculty of Science, University of Yaounde I, Yaounde I, Cameroon
Likeng-Li-Ngue Benoit Constant

Genetic and Plant Improvement Unit, Department of Plant Biology, Faculty of Science, University of Yaounde I, Yaounde I, Cameroon
Ngalle Hermine Bille

Genetic and Plant Improvement Unit, Department of Plant Biology, Faculty of Science, University of Yaounde I, Yaounde I, Cameroon
Nyouma Achille

Plant Biology and Physiology Laboratory, Department of Botany, Faculty of Science, University of Douala, Douala, Cameroon
Mbo Nkoulou Luther Fort

Plant Production Unit, Mbalmayo Agricultural Research Centre (CRAM), Institute of Agricultural Research for Development, Mbalmayo, Cameroon
Eya’a Ngombo Clement

Lipids Analysis Laboratory, Institute of Agricultural Research for Development, Specialized Station for Oil Palm of La Dibamba, Douala, Cameroon
Bell Joseph Martin

Genetic and Plant Improvement Unit, Department of Plant Biology, Faculty of Science, University of Yaounde I, Yaounde I, Cameroon

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APA Style

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, et al. (2023). Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. International Journal of Genetics and Genomics, 11(1), 1-5. https://doi.org/10.11648/j.ijgg.20231101.11

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ACS Style

Molo Thierry; Likeng-Li-Ngue Benoit Constant; Ngalle Hermine Bille; Nyouma Achille; Mbo Nkoulou Luther Fort, et al. Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. Int. J. Genet. Genomics 2023, 11(1), 1-5. doi: 10.11648/j.ijgg.20231101.11

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AMA Style

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, et al. Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. Int J Genet Genomics. 2023;11(1):1-5. doi: 10.11648/j.ijgg.20231101.11

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@article{10.11648/j.ijgg.20231101.11,
  author = {Molo Thierry and Likeng-Li-Ngue Benoit Constant and Ngalle Hermine Bille and Nyouma Achille and Mbo Nkoulou Luther Fort and Eya’a Ngombo Clement and Bell Joseph Martin},
  title = {Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization},
  journal = {International Journal of Genetics and Genomics},
  volume = {11},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ijgg.20231101.11},
  url = {https://doi.org/10.11648/j.ijgg.20231101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20231101.11},
  abstract = {The introduction of transgenic varieties of cotton (Gossypium hirsitum) in Cameroon has posed numerous concerns. One of the most concern was the horizontal transfer of the Bt gene to related species such as Abelmoschus esculentus. The current study was conducted to assess the occurrence Bt gene transfer from Gossypium hirsitum to Abelmoschus esculentus. To achieve this, the stigma receptivity periods and the viability of pollen grains were evaluated. The fruit set rates of 675 controlled intra- and interspecific crosses were assessed. The results showed that stigma receptivity and pollen grain viability are optimal at anthesis in both species, with a longer duration over 24 hours in G. hirsitum. Intra-specific crosses within the two species have shown fruit set rates of nearly 60-80%. Interspecific crosses gave a fruit set rate close to 2%, especially when A. esculentus is used as the female parent, and zero results in reciprocal crosses. The finding of the study pointed out that the possibility of horizontal transfer of a transgene to cotton relatives. In future studies, it would be useful to monitor the fate of seeds from interspecific crosses between both species and assess the agro-morphological and molecular characteristics of these possible hybrids.},
 year = {2023}
}

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