Vaccine Design for Marburg Virus Using VP35 Protein

Mohammed Yousif Mohammed; Mona Abdelrhman Mohamed Khaier; Nuha Agabna; Sania Shaddad

doi:doi:10.11648/j.ijgg.20221004.11

| Peer-Reviewed

Vaccine Design for Marburg Virus Using VP35 Protein

Mohammed Yousif Mohammed, Mona Abdelrhman Mohamed Khaier, Nuha Agabna, Sania Shaddad

Published in International Journal of Genetics and Genomics (Volume 10, Issue 4)

Received: 22 October 2022 Accepted: 8 December 2022 Published: 28 December 2022

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.

Published in	International Journal of Genetics and Genomics (Volume 10, Issue 4)
DOI	10.11648/j.ijgg.20221004.11
Page(s)	85-93
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

NTCP, ABCpred, NetMHC, Ellipro

References

[1]	Adjemian J, Farnon EC, Tschioko F, et al. Outbreak of Marburg hemorrhagic fever among miners in Kamwenge and Ibanda districts, Uganda, 2007. Journal of Infectious Diseases. 2011; 204 (Suppl 3): S796-99.
[2]	Amman BR, Carroll SA, Reed ZD, et al. Seasonal pulses of Marburg virus circulation in juvenile Rousettus aegyptiacus bats coincide with periods of increased risk of human iInfection. PLoS Pathogens. 2012; 8 (10): e1002877.
[3]	Bausch DG, Borchert M, Grein T, et al. Risk Factors for Marburg Hemorrhagic Fever, Democratic Republic of the Congo. Emerging Infectious Diseases. 2003; 9 (12): 1531-1537.
[4]	Bausch DG, Geisbert TW. Development of vaccines for Marburg hemorrhagic fever. Expert Review of Vaccines. 2007; 6 (1): 57-74.
[5]	Bausch DG, Nichol ST, Muyembe-Tamfum JJ, et al. Marburg hemorrhagic fever associated with multiple genetic lineages of virus. New England Journal of Medicine. 2006; 355: 9099-19.
[6]	Christian Sänger, Elke Mühlberger, Elena Ryabchikova, Larissa Kolesnikova, Hans-Dieter Klenk, and Stephan Becker Author information Article notes Copyright and License information Disclaimer. Sorting of Marburg Virus Surface Protein and Virus Release Take Place at Opposite Surfaces of Infected Polarized Epithelial Cells. J Virol. 2001 Feb; 75 (3): 1274–1283. doi: 10.1128/JVI.75.3.1274-1283.2001.
[7]	Messaoudi, I., Amarasinghe, G. & Basler, C. Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus. Nat Rev Microbiol 13, 663–676 (2015). https://doi.org/10.1038/nrmicro3524
[8]	Saha, S and Raghava G. P. S. (2006) Prediction of Continuous B-cell Epitopes in an Antigen Using Recurrent Neural Network. Proteins, 65 (1), 40-48 PMID: 16894596.
[9]	Nielsen, M., Andreatta, M. NetMHCpan-3.0; improved prediction of binding to MHC class I molecules integrating information from multiple receptor and peptide length datasets. Genome Med 8, 33 (2016). https://doi.org/10.1186/s13073-016-0288-x
[10]	Ponomarenko JV, Bui H, Li W, Fusseder N, Bourne PE, Sette A, Peters B. 2008. ElliPro: a new structure-based tool for the prediction of antibody epitopes. BMC Bioinformatics 9: 514. PMID: 19055730.
[11]	Irini A Doytchinova and Darren R Flower. VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC Bioinformatics. 2007 8: 4. Irini A Doytchinova and Darren R Flower. Identifying candidate subunit vaccines using an alignment-independent method based on principal amino acid properties. Vaccine. 2007 25: 856-866. Irini A Doytchinova and Darren R Flower. Bioinformatic Approach for Identifying Parasite and Fungal Candidate Subunit Vaccines. Open Vaccines Journal, 2008 1: 22-26.
[12]	Dimitrov, I., Flower, D. R. & Doytchinova, I. AllerTOP - a server for in silico prediction of allergens. BMC Bioinformatics 14, S4 (2013). https://doi.org/10.1186/1471-2105-14-S6-S4
[13]	Sudheer Gupta Pallavi Kapoor, Kumardeep Chaudhary, Ankur Gautam, Rahu Kumar Open Source Drug Discovery Consortium, Gajendra P. S. Raghav, In Silico Approach for Predicting Toxicity of Peptides and Proteins Published: September 13, 2013 https://doi.org/10.1371/journal.pone.0073957
[14]	ACD/ChemSketch, version 2021.1.1, Advanced Chemistry Development, Inc., Toronto, ON, Canada, www.acdlabs.com, 2021.
[15]	O'Boyle, N. M., Banck, M., James, C. A. et al. Open Babel: An open chemical toolbox. J Cheminform 3, 33 (2011). https://doi.org/10.1186/1758-2946-3-33
[16]	Grosdidier A, Zoete V, Michielin O. SwissDock, a protein-small molecule docking web service based on EADock DSS. Nucleic Acids Res. 2011; 39 (Web Server issue): W270-W277. doi: 10.1093/nar/gkr366.
[17]	Saad Ahmed Sami, Kay Kay Shain Marma, Shafi Mahmud, Md. Asif Nadim Khan, Sarah Albogami, Ahmed M. El-Shehawi, Ahmed Rakib, Agnila Chakraborty, Mostafah Mohiuddin, Kuldeep Dhama, Mir Muhammad Nasir Uddin, Mohammed Kamrul HossainTrina Ekawati Talle, and Talha Bin Emran Designing of a Multi-epitope Vaccine against the Structural Proteins of Marburg Virus Exploiting the Immunoinformatics Approach, ACS Omega 2021, 6, 47, 32043–32071 Publication Date: November 18, 2021. https://doi.org/10.1021/acsomega.1c04817 Copyright © 2021 The Authors. Published by American Chemical Society.
[18]	Sameer Quazi, Javed Malik, Arnaud Martino Capuzzo, Kamal Singh Suman, Zeshan Haider, In-silico Structural and Molecular Docking-Based Drug Discovery Against Viral Protein (VP35) of Marburg Virus: A potent Agent of MAVD, doi: https://doi.org/10.1101/2021.02.09.430405.
[19]	AnupamDhasmana^abSwatiDhasmana^aAhmadAlsulimani^cSudhirKotnala^aVivekKumarKashyap^aShafiulHaque^deMeenaJaggi^aMurali M. Yallapu^aSubhash C. Chauhan, In silico CD4 + T-cell multiepitope prediction and HLA distribution analysis for Marburg Virus—A strategy for vaccine designing, https://doi.org/10.1016/j.jksus.2021.101751

Cite This Article

Plain Text BibTeX RIS

APA Style

Mohammed Yousif Mohammed, Mona Abdelrhman Mohamed Khaier, Nuha Agabna, Sania Shaddad. (2022). Vaccine Design for Marburg Virus Using VP35 Protein. International Journal of Genetics and Genomics, 10(4), 85-93. https://doi.org/10.11648/j.ijgg.20221004.11

Copy | Download

ACS Style

Mohammed Yousif Mohammed; Mona Abdelrhman Mohamed Khaier; Nuha Agabna; Sania Shaddad. Vaccine Design for Marburg Virus Using VP35 Protein. Int. J. Genet. Genomics 2022, 10(4), 85-93. doi: 10.11648/j.ijgg.20221004.11

Copy | Download

AMA Style

Mohammed Yousif Mohammed, Mona Abdelrhman Mohamed Khaier, Nuha Agabna, Sania Shaddad. Vaccine Design for Marburg Virus Using VP35 Protein. Int J Genet Genomics. 2022;10(4):85-93. doi: 10.11648/j.ijgg.20221004.11

Copy | Download

@article{10.11648/j.ijgg.20221004.11,
  author = {Mohammed Yousif Mohammed and Mona Abdelrhman Mohamed Khaier and Nuha Agabna and Sania Shaddad},
  title = {Vaccine Design for Marburg Virus Using VP35 Protein},
  journal = {International Journal of Genetics and Genomics},
  volume = {10},
  number = {4},
  pages = {85-93},
  doi = {10.11648/j.ijgg.20221004.11},
  url = {https://doi.org/10.11648/j.ijgg.20221004.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20221004.11},
  abstract = {Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Vaccine Design for Marburg Virus Using VP35 Protein
AU  - Mohammed Yousif Mohammed
AU  - Mona Abdelrhman Mohamed Khaier
AU  - Nuha Agabna
AU  - Sania Shaddad
Y1  - 2022/12/28
PY  - 2022
N1  - https://doi.org/10.11648/j.ijgg.20221004.11
DO  - 10.11648/j.ijgg.20221004.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 85
EP  - 93
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20221004.11
AB  - Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.
VL  - 10
IS  - 4
ER  -

Copy | Download

Author Information

Mohammed Yousif Mohammed

Department of Biotechnology, Faculty of Industrial and Applied Science, University of Bahri, Khartoum, Sudan
Mona Abdelrhman Mohamed Khaier

Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan
Nuha Agabna

Department of Pharmacology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
Sania Shaddad

Department of Pharmacology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Mohammed Yousif Mohammed, Mona Abdelrhman Mohamed Khaier, Nuha Agabna, Sania Shaddad. (2022). Vaccine Design for Marburg Virus Using VP35 Protein. International Journal of Genetics and Genomics, 10(4), 85-93. https://doi.org/10.11648/j.ijgg.20221004.11

Copy | Download

ACS Style

Mohammed Yousif Mohammed; Mona Abdelrhman Mohamed Khaier; Nuha Agabna; Sania Shaddad. Vaccine Design for Marburg Virus Using VP35 Protein. Int. J. Genet. Genomics 2022, 10(4), 85-93. doi: 10.11648/j.ijgg.20221004.11

Copy | Download

AMA Style

Mohammed Yousif Mohammed, Mona Abdelrhman Mohamed Khaier, Nuha Agabna, Sania Shaddad. Vaccine Design for Marburg Virus Using VP35 Protein. Int J Genet Genomics. 2022;10(4):85-93. doi: 10.11648/j.ijgg.20221004.11

Copy | Download

@article{10.11648/j.ijgg.20221004.11,
  author = {Mohammed Yousif Mohammed and Mona Abdelrhman Mohamed Khaier and Nuha Agabna and Sania Shaddad},
  title = {Vaccine Design for Marburg Virus Using VP35 Protein},
  journal = {International Journal of Genetics and Genomics},
  volume = {10},
  number = {4},
  pages = {85-93},
  doi = {10.11648/j.ijgg.20221004.11},
  url = {https://doi.org/10.11648/j.ijgg.20221004.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20221004.11},
  abstract = {Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Vaccine Design for Marburg Virus Using VP35 Protein
AU  - Mohammed Yousif Mohammed
AU  - Mona Abdelrhman Mohamed Khaier
AU  - Nuha Agabna
AU  - Sania Shaddad
Y1  - 2022/12/28
PY  - 2022
N1  - https://doi.org/10.11648/j.ijgg.20221004.11
DO  - 10.11648/j.ijgg.20221004.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 85
EP  - 93
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20221004.11
AB  - Background: Marburg virus is cause hemorrhagic fever for human, and transmits by exposure to one species of fruit bats, and transmits from person to person by body fluid it cause fever and bleeding but not like Ebola virus symptoms. Aim: in this study use bioinformatics tools to design a vaccine using vp35 protein which is part of virus cell that can help to prevent Marburg virus from spreading. Material and Method: after retrieval sequence of VP35 protein from National center for biotechnology information (NCBI) ABCpred were used to indicate B-cell epitopes and NetMHc to identify T-cell epitopes. To identify antibody prediction epitopes for linear and discontinuous IEDP Elipro tools and their 3D structure was used. To identify allerginicity Allertop server and vaxijen 2 servers were used for the toxicity and Toxinpred was used. Results: a 32 B-cell epitopes and 72 T-cell epitopes with high conservancy epitopes, and no allergy mentioned and toxicity, also the 3D structure represent the predicted epitope vaccine in the two way discontinuous and linear one. Conclusion: Marburg virus is a very limited outbreak virus but once it start to infect someone, it will start to spread powerfully to infect many, due to its process of infection plus its dangerous that no treatment yet and even no vaccine, some scientists link Marburg virus with Ebola because they have the same symptoms and the same way of infection and source which is the fruit bat, but still commonly different in many ways but the complications remain the same. This designed vaccine can help and prevent people specially where disease outbreak.
VL  - 10
IS  - 4
ER  -

Copy | Download