Bats are nocturnal animals with functional eyes and M/L and S opsin genes in the majority of the species. These genes are prerequisite for daylight, UV and dichromatic colour vision. Several studies suggest that other non-visual light-sensitive pigments are also involved in the UV light perception in animals. Recent behavioural, molecular and immunohistochemical evidence supports that the opsin-like gene, neuropsin (OPN5), is identified in humans, mice and birds, where it serves as a G protein-coupled UV-sensitive photoreceptor. Based on its low sequence homology with other opsin groups, OPN5 is classified as an independent group. While the roles of non-visual light-sensitive pigment OPN5 in bats remain an open question, here we report that bat’s neuropsin (OPN5) encoded by OPN5 gene shares 89-96% amino acid identity and similar domain organization with human and mouse OPN5. By PCR amplification, we confirm that all the mega and micro bats express the OPN5 gene in their genome. The expression of OPN5 is detectable only in the brain, eye and retina and not in the heart, kidney, liver, lungs and testis. This result suggests that OPN5 gene expression is neural specific in bats. OPN5 gene expression level is significantly higher in tree-roosting bats compared to cave-roosting bats. Since, the tree-roosting bats received slightly more sunlight every day when compared to cave-roosting bats. In captive conditions, the expression levels of OPN5 in the neural tissues are significantly lower than those of wild bats. Our preliminary results suggest that the opsin-like gene, neuropsin (OPN5) is involved in UV light perception in bats.
| Published in | International Journal of Genetics and Genomics (Volume 10, Issue 1) |
| DOI | 10.11648/j.ijgg.20221001.14 |
| Page(s) | 21-31 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Bats, UV-vision, Opsin Genes, Non-visual Pigments, Neuropsin (OPN5)
| [1] | Kefalov, V. J. (2012). Rod and cone visual pigments and phototransduction through pharmacological, genetic, and physiological approaches. Journal of Biological Chemistry, 287: 1635-1641. |
| [2] | Fujun, X., Kailiang, H., Tengteng, Z., Paul, R., Xuzhong, W. and Yi, S. (2012). Behavioral evidence for cone-based ultraviolet vision in divergent bat species and implications for its evolution. Zoologia (Curitiba), 292: 109-114. |
| [3] | Shichida, Y. and Matsuyama, T. (2009). Evolution of opsins and phototransduction. Philosophical Transactions of the Royal Society B: Biological Sciences, 364: 2881–2895. |
| [4] | Yau, K. W. (1994). Phototransduction mechanism in retinal rods and cones. Investigative Ophthalmology and Visual Science, 35: 9-32. |
| [5] | Ahnelt, P. K. and Kolb, H. (2000). The mammalian photoreceptor mosaic-adaptive design. Progress in Retinal and Eye Research, 19: 711-777. |
| [6] | Cronin, T. W. and Bok, M. J. (2016). Photoreception and vision in the ultraviolet. Journal of Experimental Biology, 219: 2790-2801. |
| [7] | Eisner, T., Silberglied, R. E., Aneshansley, D., Carrel, J. E. and Howland, H. C. (1969). Ultraviolet video viewing: the television camera as an insect eye. Science, 166: 1172–1174. |
| [8] | Froy, O., Gotter, A. L., Casselman, A. L. and Reppert, S. M. (2003). Illuminating the circadian clock in monarch butterfly migration. Science, 300: 1303–1305. |
| [9] | Kojima, D., Mori, S., Torii, M., Wada, A., Morishita, R. and Fukada, Y. (2011). UV-Sensitive Photoreceptor Protein OPN5 in Humans and Mice. PLoS One, 6: e26388. |
| [10] | Yokoyama, S. (1997). Molecular genetic basis of adaptive selection: examples from color vision in vertebrates. Annual Review of Genetics, 31: 315-336. |
| [11] | Li, L., Chi, H., Liu, H., Xia, Y., Irwin, D. M., Zhang, S. and Liu, Y. (2018). Retention and losses of ultraviolet-sensitive visual pigments in bats. Scientific Reports, 8 (1): 11933. doi: 10.1038/s41598-018-29646-6. |
| [12] | Terakita, A. (2005). The opsins. Genome Biology, 6: 213.1-213.9. |
| [13] | Unger, V. M. and Schertler, G. F. (1995). Low resolution structure of bovine rhodopsin determined by electron cryo-microscopy. Biophysical Journal, 68: 1776-1786. |
| [14] | Muller, B., Butz, E., Peichl, L. and Haverkamp, S. (2013). The rod pathway of the microbat retina has bistratified rod bipolar cells and tristratified all amacrine cells. Journal of Neuroscience, 33: 1014-1023. |
| [15] | Eklof, J. and Jones, G. (2003). Use of vision in prey detection by brown long-eared bats, Plecotus auritus. Animal Behaviour, 66: 949-953. |
| [16] | Speakman, J. (2001). The evolution of flight and echolocation in bats: another leap in the dark. Mammal Review, 31: 111-130. |
| [17] | Zhao, H. B., Rossiter, S. J., Teeling, E. C., Li, C. J., Cotton, J. A. and Zhang, S. Y. (2009). The evolution of color vision in nocturnal mammals. Proceedings of the National Academy of Sciences, 106: 8980-8985. |
| [18] | Kim, T. J., Jeon, Y. K., Lee, J. Y., Lee, E. S. and Jeon, C. J. (2008). The photoreceptor populations in the retina of the greater horseshoe bat Rhinolophus ferrumequinum. Molecules and Cells, 26: 373-379. |
| [19] | Muller, B., Glosmann, M., Peichl, L., Knop, G. C., Hagemann, C. and Ammermuller, J. (2009). Bat eyes have ultraviolet-sensitive cone photoreceptors. PLoS One, 4: e6390 doi: 10.1371/journal.pone.0006390. |
| [20] | Griffin, D. R. (1970). Migration and homing of bats. In: Biology of bats (Wimsatt WA, ed) New York: Academic, p. 233–264. |
| [21] | Suthers, R. A. (1970). Vision, olfaction, taste. In: Biology of bats (Wimsatt WA, eds) New York: Academic, p. 265-304. |
| [22] | Eklof, J. (2003). Vision in echolocating bats. Doctoral thesis. Department of Zoology, Göteborg University, Sweden. |
| [23] | Altringham, J. D. and Fenton, M. B. (2003). Sensory ecology and communication in the chiroptera. In: Bat ecology (Kunz TH, Fenton MB, eds), Chicago: The University of Chicago Press, p. 90-127. |
| [24] | Yamashita, T., Ono, K., Ohuchi, H., Yumoto, A., Gotoh, H. and Tomonari, S. et. al. (2014). Evolution of mammalian OPN5 as a specialized UV-absorbing pigment by a single amino acid mutation. Journal of Biological Chemistry, 289: 3991-4000. |
| [25] | Tarttelin, E. E., Bellingham, J., Hankins, M. W., Foster, R. G. and Lucas, R. J. (2003). Neuropsin (OPN5): a novel opsin identified in mammalian neural tissue. Federation of European Biochemical Societies Letter, 554: 410-416. |
| [26] | Ohuchi, H., Yamashita, T., Tomonari, S., Fujita-Yanagibayashi, S., Sakai, K., Noji, S. and Shichida, Y. (2012). A non-mammalian type opsin 5 functions dually in the photoreceptive and non-photoreceptive organs of birds. PLoS One, 7: e31534. |
| [27] | Tomonari, S., Migita, K., Takagi, A., Noji, S. and Ohuchi, H. (2008). Expression patterns of the opsin 5–related genes in the developing chicken retina. Developmental Dynamics, 237: 1910–1922. |
| [28] | Terakita, A., Koyanagi, M., Tsukamoto, H., Yamashita, T., Miyata, T. and Shichida, Y. (2004). Counterion displacement in the molecular evolution of the rhodopsin family. Nature Structural and Molecular Biology, 11: 284-289. |
| [29] | Gaisler, J. (1973). Netting as possible approach to study bat activity. Periodicum Biologorum. 75: 129-134. |
| [30] | Karuppudurai, T., Sripathi, K., Gopukumar, N., Elangovan, V., Marimuthu, G. (2007). Genetic diversity within and among populations of the Indian short-nosed fruit bat Cynopterus sphinx assessed through RAPD analysis. Current Science, 93: 942-950. |
| [31] | Wilmer, J. W., Barratt, E. (1996). A non-lethal method of tissue sampling for genetic studies of chiropterans. Bat Research News, 37: 1-3. |
| [32] | Karuppudurai, T. and Sripathi, K. (2010). Molecular genetic analysis of male alternative strategy and reproductive success in the polygynous mating bat Cynopterus sphinx. Journal of Cell and Animal Biology, 4: 042–050. |
| [33] | Upton, B. A., Díaz, N. M., Gordon, S. A., Van Gelder, R. N., Buhr, E. D. and Lang, R. A (2021). Evolutionary constraint on visual and non-visual mammalian opsins. Journal of Biological Rhythms, 36: 109-126. |
| [34] | Schwartz, G. J. (2020). Light-activated neurons can alter body heat. Nature, 585: 351-352. |
| [35] | Hauser, F. E. and Chang, B. S. W. (2017). Insights into visual pigment adaptation and diversity from model ecological and evolutionary systems. Current Opinion in Genetics and Development, 47: 110–120. |
| [36] | Buhr, E. D., Vemaraju, S., Diaz, N., Lang, R. A. and Van Gelder, R. N. (2019). Neuropsin (OPN5) mediates local light-dependent induction of circadian clock genes and circadian photoentrainment in exposed murine skin. Current Biology, 29: 3478–3487. |
| [37] | Suh, S., Choi, E. H. and Mesinkovska, N. A. (2020). The expression of opsins in the human skin and its implications for photobiomodulation: A Systematic Review. Photodermatology Photoimmunology and Photomedicine, 36: 329-338. |
| [38] | Nakane, Y., Ikegami, K., Ono, H., Yamamoto, N., Yoshida, S., Hirunagi, K. et. al. (2010). A mammalian neural tissue opsin (Opsin 5) is a deep brain photoreceptor in birds. Proceedings of the National Academy of Sciences, 107: 15264–15268. |
| [39] | Nguyen, M. T., Vemaraju, S., Nayak, G., Odaka, Y., Buhr, E. D. et al. (2019). An Opsin 5-dopamine pathway mediates light-dependent vascular development in the eye (2019). Nature Cell Biology, 21: 420–429. |
| [40] | Zhang, K. Z., D’Souza, S., Upton, B. A., Kernodle, S., Vemaraju, S., et. al. (2020). Violet-light suppression of thermogenesis by opsin 5 hypothalamic neurons. Nature, 585: 420-425. |
| [41] | Yamashita, T., Ohuchi, H., Tomonari, S., Ikeda, K., Sakai, K. and Shichida, Y. (2010). OPN5 is a UV-sensitive bistable pigment that couples with Gi subtype of G protein. Proceedings of the National Academy of Sciences, 107: 22084-22089. |
| [42] | Winter, Y., López, J. and von Helversen, O. (2003). Ultraviolet vision in a bat. Nature, 425, 612-614. |
| [43] | Lythgoe, J. N. (1979). The ecology of vision. Oxford, Clarendon Press, New York, Oxford University Press, pp. 244. |
APA Style
Steffi Christiane Ramesh, Subbian Baskaran, Uthandakalaipandian Ramesh, Thangavel Karuppudurai. (2022). Neuropsin (OPN5): A Non-visual UV Sensitive Photoreceptor Gene Expression Pattern Among Mega and Micro Bats. International Journal of Genetics and Genomics, 10(1), 21-31. https://doi.org/10.11648/j.ijgg.20221001.14
ACS Style
Steffi Christiane Ramesh; Subbian Baskaran; Uthandakalaipandian Ramesh; Thangavel Karuppudurai. Neuropsin (OPN5): A Non-visual UV Sensitive Photoreceptor Gene Expression Pattern Among Mega and Micro Bats. Int. J. Genet. Genomics 2022, 10(1), 21-31. doi: 10.11648/j.ijgg.20221001.14
AMA Style
Steffi Christiane Ramesh, Subbian Baskaran, Uthandakalaipandian Ramesh, Thangavel Karuppudurai. Neuropsin (OPN5): A Non-visual UV Sensitive Photoreceptor Gene Expression Pattern Among Mega and Micro Bats. Int J Genet Genomics. 2022;10(1):21-31. doi: 10.11648/j.ijgg.20221001.14
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Download@article{10.11648/j.ijgg.20221001.14,
author = {Steffi Christiane Ramesh and Subbian Baskaran and Uthandakalaipandian Ramesh and Thangavel Karuppudurai},
title = {Neuropsin (OPN5): A Non-visual UV Sensitive Photoreceptor Gene Expression Pattern Among Mega and Micro Bats},
journal = {International Journal of Genetics and Genomics},
volume = {10},
number = {1},
pages = {21-31},
doi = {10.11648/j.ijgg.20221001.14},
url = {https://doi.org/10.11648/j.ijgg.20221001.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20221001.14},
abstract = {Bats are nocturnal animals with functional eyes and M/L and S opsin genes in the majority of the species. These genes are prerequisite for daylight, UV and dichromatic colour vision. Several studies suggest that other non-visual light-sensitive pigments are also involved in the UV light perception in animals. Recent behavioural, molecular and immunohistochemical evidence supports that the opsin-like gene, neuropsin (OPN5), is identified in humans, mice and birds, where it serves as a G protein-coupled UV-sensitive photoreceptor. Based on its low sequence homology with other opsin groups, OPN5 is classified as an independent group. While the roles of non-visual light-sensitive pigment OPN5 in bats remain an open question, here we report that bat’s neuropsin (OPN5) encoded by OPN5 gene shares 89-96% amino acid identity and similar domain organization with human and mouse OPN5. By PCR amplification, we confirm that all the mega and micro bats express the OPN5 gene in their genome. The expression of OPN5 is detectable only in the brain, eye and retina and not in the heart, kidney, liver, lungs and testis. This result suggests that OPN5 gene expression is neural specific in bats. OPN5 gene expression level is significantly higher in tree-roosting bats compared to cave-roosting bats. Since, the tree-roosting bats received slightly more sunlight every day when compared to cave-roosting bats. In captive conditions, the expression levels of OPN5 in the neural tissues are significantly lower than those of wild bats. Our preliminary results suggest that the opsin-like gene, neuropsin (OPN5) is involved in UV light perception in bats.},
year = {2022}
}
TY - JOUR T1 - Neuropsin (OPN5): A Non-visual UV Sensitive Photoreceptor Gene Expression Pattern Among Mega and Micro Bats AU - Steffi Christiane Ramesh AU - Subbian Baskaran AU - Uthandakalaipandian Ramesh AU - Thangavel Karuppudurai Y1 - 2022/02/19 PY - 2022 N1 - https://doi.org/10.11648/j.ijgg.20221001.14 DO - 10.11648/j.ijgg.20221001.14 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 21 EP - 31 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20221001.14 AB - Bats are nocturnal animals with functional eyes and M/L and S opsin genes in the majority of the species. These genes are prerequisite for daylight, UV and dichromatic colour vision. Several studies suggest that other non-visual light-sensitive pigments are also involved in the UV light perception in animals. Recent behavioural, molecular and immunohistochemical evidence supports that the opsin-like gene, neuropsin (OPN5), is identified in humans, mice and birds, where it serves as a G protein-coupled UV-sensitive photoreceptor. Based on its low sequence homology with other opsin groups, OPN5 is classified as an independent group. While the roles of non-visual light-sensitive pigment OPN5 in bats remain an open question, here we report that bat’s neuropsin (OPN5) encoded by OPN5 gene shares 89-96% amino acid identity and similar domain organization with human and mouse OPN5. By PCR amplification, we confirm that all the mega and micro bats express the OPN5 gene in their genome. The expression of OPN5 is detectable only in the brain, eye and retina and not in the heart, kidney, liver, lungs and testis. This result suggests that OPN5 gene expression is neural specific in bats. OPN5 gene expression level is significantly higher in tree-roosting bats compared to cave-roosting bats. Since, the tree-roosting bats received slightly more sunlight every day when compared to cave-roosting bats. In captive conditions, the expression levels of OPN5 in the neural tissues are significantly lower than those of wild bats. Our preliminary results suggest that the opsin-like gene, neuropsin (OPN5) is involved in UV light perception in bats. VL - 10 IS - 1 ER -
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