Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province

Zhang Weiyu; Liu Guihua; Huang Xin; Zhang Lifu; Yao Jiatong

doi:doi:10.11648/j.ijfsb.20220701.15

| Peer-Reviewed

Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province

Zhang Weiyu, Liu Guihua, Huang Xin, Zhang Lifu, Yao Jiatong

Published in International Journal of Food Science and Biotechnology (Volume 7, Issue 1)

Received: 2 March 2022 Accepted: 17 March 2022 Published: 24 March 2022

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Objective: To detec integron genes and the drug resistance of two foodborne salmonella from different sources in Jilin Province, explore the correlation between drug resistance and integron. Method: The minimum inhibitory concentration (MIC) method was used to detect drug resistance of 278 salmonella strains in Jilin Province. Real-time PCR was used to detect type I, II and III integron genes, and the correlation between drug resistance and integron gene carrier rate was compared. Result: The total drug resistance rate of 278 strains of food-borne Salmonella was 89.57% (249/278). The positive rates of type I integron, type II integron and type III integron were 51.44% (143/278), 4.32% (12/278) and 14.39% (40/278) respectively. The drug resistance rate of foodborne disease samples was 95.8% (207/216), type I integron was 58.8% (127/216), type II integron was 5.56% (12/216), and type III integron gene was 17.59% (38/216). The drug resistance rate of food (meat, egg) samples was 67.7% (42/62), type I integron was 25.81% (16/62), type III integron gene was 3.23% (2/62), and type II integron gene was detected. Conclusion: Through the surveillance of Salmonella drug resistance and Integron gene carrying rate in food safety risk surveillance and food-borne disease surveillance in Jilin Province, The gene carrying rate of type I and III Integron of Salmonella from food-borne diseases was significantly higher than that of food (meat and eggs). Integron gene system plays an important role in drug resistance transmission of foodborne pathogens. It is suggested that attention should be paid to the monitoring of salmonella drug resistance to ensure food safety and human health.

Published in	International Journal of Food Science and Biotechnology (Volume 7, Issue 1)
DOI	10.11648/j.ijfsb.20220701.15
Page(s)	16-20
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Food Safety, Salmonella, Drug Resistance Monitoring, Integron Genes

References

[1]	Jansen W, Muller A, Grabowski N T, et al. Foodborne diseases do not respect borders: zoonotic pathogens ant antimicrobialresistant bacterianin food products of animai origin illegally imported into the European Union [J]. The Veternary Journal, 2019, 244: 75-82.
[2]	Zheng L, Zhu LW, Guo XJ, Chen P. Research progress of antimicrobial resistance of major epidemic serotypes of Salmonella. Jiangsu Agricultural Sciences, 2020, 48 (6): 8–12. (in Chinese).
[3]	Timothy M. Ghaly; Jemma L. Geoghegan; Sasha G. Tetu; Michael R. Gillings. The Peril and Promise of Integrons [J]. Beyond Antibiotic Resistance. Trends in MicrobiologyVolume, 2020, 28 (6): 455-464.
[4]	Liyu, xiaojiuan Yang, et, al. Research Progress of integron-mediated drug resistance of Salmonella [J]. Acta Microbiologica Sinica, 2021, 61 (12): 3918–3927.
[5]	DU X D, LI D X, HU G Z, et al. Tn1548-associated armA is colocated with qnrB2, aac (6') -Ib-cr and blaCTX-M- 3 on an IncF II plasmid in a Salmonella enterica subsp. enterica serovar paratyphiB strain isolated from chickens in China [J]. J AntimicrobChemother, 2012, 67 (1): 246-248.
[6]	Gharieb R M, Tartor Y H, Khedr M H. Non-typhoidal Salmonella in poultry meat and diarrhoeic patients: prevalence, antibiogram, virulotyping, molecular detection and sequencing of class Iintegrons in multidrug resistant strains [J] Gut Pathog, 2015, 7 (1): 1-11.
[7]	Feng Y, Yuan JL, Wu Y, Qi Y, Chen T. Using reverse PCR to analysis flanking DNA regions of class I integrons. International Journal of Laboratory Medicine, 2013, 34 (8): 919–920. (in Chinese).
[8]	Xu JX, Jia XB, Shen HY, Xu M, Dou XH. Study on detection of bacterial integrons by multiple LAMP. China Poultry, 2019, 41 (14): 64–66. (in Chinese).
[9]	Antunes P, Mourao J, Campos J, et al. Salmonellosis: the role of poultry meat [J]. Clin Microbiol Infect. 2016, 22 (2): 110-121.
[10]	Ben Salem R, Abbassi M S, Garcia, Vanese, et al. Antimicrobial drug resistance and genetic properties of Salmonella enteric serotype Enteritidis circulating in chicken farms in Tunisia [J]. Journal of Infection and Public Health, 2017, 10 (6): 855-860.
[11]	Liu Guihua, Huang Xin, Zhang Weiyu, Wang Yanqiu. Detection and analysis of antimicrobial resistance of Salmonella in faeces of foodborne disease patients [J]. Science Journal of Public Health, 2020, 8 (3): 77-82.
[12]	European Food Safety Authority; European Centre for Disease Prevention and Control. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017. EFSA J. 2019; 17 (2): e05598. Published 2019 Feb 27.
[13]	Xiaojuan Yang; Jiahui Huang; Youxiong Zhang; Shengrong Liu; et al. Prevalence, abundance, serovars and antimicrobial resistance of Salmonella isolated from retail raw poultry meat in China [J]. Science of The Total Environment Volume 713, Issue C. 2020. PP 136385.
[14]	Zhang Meng, LI Yan-fen, QI Hao-yu, Zhang Guang-wei, Qiu Zheng-yong, Zhang Xiu-li. Surveilance situation of Salmonella in foodborne diseases in Henan, china 2015-2016 [J]. Chinese Journal of Zoonoses. 2017, 33 (08): 748-752.
[15]	Yaochuan Liu, benliang Yu, et al. Progress in the study of drug resistance transmission mechanism in integron-cassettes system [J]. Chinese Journal of Preventive Veterinary, 2017, 39 (03): 244-247.

Cite This Article

Plain Text BibTeX RIS

APA Style

Zhang Weiyu, Liu Guihua, Huang Xin, Zhang Lifu, Yao Jiatong. (2022). Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province. International Journal of Food Science and Biotechnology, 7(1), 16-20. https://doi.org/10.11648/j.ijfsb.20220701.15

Copy | Download

ACS Style

Zhang Weiyu; Liu Guihua; Huang Xin; Zhang Lifu; Yao Jiatong. Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province. Int. J. Food Sci. Biotechnol. 2022, 7(1), 16-20. doi: 10.11648/j.ijfsb.20220701.15

Copy | Download

AMA Style

Zhang Weiyu, Liu Guihua, Huang Xin, Zhang Lifu, Yao Jiatong. Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province. Int J Food Sci Biotechnol. 2022;7(1):16-20. doi: 10.11648/j.ijfsb.20220701.15

Copy | Download

@article{10.11648/j.ijfsb.20220701.15,
  author = {Zhang Weiyu and Liu Guihua and Huang Xin and Zhang Lifu and Yao Jiatong},
  title = {Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {7},
  number = {1},
  pages = {16-20},
  doi = {10.11648/j.ijfsb.20220701.15},
  url = {https://doi.org/10.11648/j.ijfsb.20220701.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20220701.15},
  abstract = {Objective: To detec integron genes and the drug resistance of two foodborne salmonella from different sources in Jilin Province, explore the correlation between drug resistance and integron. Method: The minimum inhibitory concentration (MIC) method was used to detect drug resistance of 278 salmonella strains in Jilin Province. Real-time PCR was used to detect type I, II and III integron genes, and the correlation between drug resistance and integron gene carrier rate was compared. Result: The total drug resistance rate of 278 strains of food-borne Salmonella was 89.57% (249/278). The positive rates of type I integron, type II integron and type III integron were 51.44% (143/278), 4.32% (12/278) and 14.39% (40/278) respectively. The drug resistance rate of foodborne disease samples was 95.8% (207/216), type I integron was 58.8% (127/216), type II integron was 5.56% (12/216), and type III integron gene was 17.59% (38/216). The drug resistance rate of food (meat, egg) samples was 67.7% (42/62), type I integron was 25.81% (16/62), type III integron gene was 3.23% (2/62), and type II integron gene was detected. Conclusion: Through the surveillance of Salmonella drug resistance and Integron gene carrying rate in food safety risk surveillance and food-borne disease surveillance in Jilin Province, The gene carrying rate of type I and III Integron of Salmonella from food-borne diseases was significantly higher than that of food (meat and eggs). Integron gene system plays an important role in drug resistance transmission of foodborne pathogens. It is suggested that attention should be paid to the monitoring of salmonella drug resistance to ensure food safety and human health.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province
AU  - Zhang Weiyu
AU  - Liu Guihua
AU  - Huang Xin
AU  - Zhang Lifu
AU  - Yao Jiatong
Y1  - 2022/03/24
PY  - 2022
N1  - https://doi.org/10.11648/j.ijfsb.20220701.15
DO  - 10.11648/j.ijfsb.20220701.15
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 16
EP  - 20
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20220701.15
AB  - Objective: To detec integron genes and the drug resistance of two foodborne salmonella from different sources in Jilin Province, explore the correlation between drug resistance and integron. Method: The minimum inhibitory concentration (MIC) method was used to detect drug resistance of 278 salmonella strains in Jilin Province. Real-time PCR was used to detect type I, II and III integron genes, and the correlation between drug resistance and integron gene carrier rate was compared. Result: The total drug resistance rate of 278 strains of food-borne Salmonella was 89.57% (249/278). The positive rates of type I integron, type II integron and type III integron were 51.44% (143/278), 4.32% (12/278) and 14.39% (40/278) respectively. The drug resistance rate of foodborne disease samples was 95.8% (207/216), type I integron was 58.8% (127/216), type II integron was 5.56% (12/216), and type III integron gene was 17.59% (38/216). The drug resistance rate of food (meat, egg) samples was 67.7% (42/62), type I integron was 25.81% (16/62), type III integron gene was 3.23% (2/62), and type II integron gene was detected. Conclusion: Through the surveillance of Salmonella drug resistance and Integron gene carrying rate in food safety risk surveillance and food-borne disease surveillance in Jilin Province, The gene carrying rate of type I and III Integron of Salmonella from food-borne diseases was significantly higher than that of food (meat and eggs). Integron gene system plays an important role in drug resistance transmission of foodborne pathogens. It is suggested that attention should be paid to the monitoring of salmonella drug resistance to ensure food safety and human health.
VL  - 7
IS  - 1
ER  -

Copy | Download

Author Information

Zhang Weiyu

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Liu Guihua

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Huang Xin

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Zhang Lifu

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China
Yao Jiatong

Institute of Microbiology Department, Jilin Provincial Center for Disease Control and Prevention, Changchun, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Zhang Weiyu, Liu Guihua, Huang Xin, Zhang Lifu, Yao Jiatong. (2022). Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province. International Journal of Food Science and Biotechnology, 7(1), 16-20. https://doi.org/10.11648/j.ijfsb.20220701.15

Copy | Download

ACS Style

Zhang Weiyu; Liu Guihua; Huang Xin; Zhang Lifu; Yao Jiatong. Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province. Int. J. Food Sci. Biotechnol. 2022, 7(1), 16-20. doi: 10.11648/j.ijfsb.20220701.15

Copy | Download

AMA Style

Zhang Weiyu, Liu Guihua, Huang Xin, Zhang Lifu, Yao Jiatong. Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province. Int J Food Sci Biotechnol. 2022;7(1):16-20. doi: 10.11648/j.ijfsb.20220701.15

Copy | Download

@article{10.11648/j.ijfsb.20220701.15,
  author = {Zhang Weiyu and Liu Guihua and Huang Xin and Zhang Lifu and Yao Jiatong},
  title = {Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {7},
  number = {1},
  pages = {16-20},
  doi = {10.11648/j.ijfsb.20220701.15},
  url = {https://doi.org/10.11648/j.ijfsb.20220701.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20220701.15},
  abstract = {Objective: To detec integron genes and the drug resistance of two foodborne salmonella from different sources in Jilin Province, explore the correlation between drug resistance and integron. Method: The minimum inhibitory concentration (MIC) method was used to detect drug resistance of 278 salmonella strains in Jilin Province. Real-time PCR was used to detect type I, II and III integron genes, and the correlation between drug resistance and integron gene carrier rate was compared. Result: The total drug resistance rate of 278 strains of food-borne Salmonella was 89.57% (249/278). The positive rates of type I integron, type II integron and type III integron were 51.44% (143/278), 4.32% (12/278) and 14.39% (40/278) respectively. The drug resistance rate of foodborne disease samples was 95.8% (207/216), type I integron was 58.8% (127/216), type II integron was 5.56% (12/216), and type III integron gene was 17.59% (38/216). The drug resistance rate of food (meat, egg) samples was 67.7% (42/62), type I integron was 25.81% (16/62), type III integron gene was 3.23% (2/62), and type II integron gene was detected. Conclusion: Through the surveillance of Salmonella drug resistance and Integron gene carrying rate in food safety risk surveillance and food-borne disease surveillance in Jilin Province, The gene carrying rate of type I and III Integron of Salmonella from food-borne diseases was significantly higher than that of food (meat and eggs). Integron gene system plays an important role in drug resistance transmission of foodborne pathogens. It is suggested that attention should be paid to the monitoring of salmonella drug resistance to ensure food safety and human health.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Correlation Analysis of Drug Resistance and Integron Gene Types of Food-borne Salmonella from Jilin Province
AU  - Zhang Weiyu
AU  - Liu Guihua
AU  - Huang Xin
AU  - Zhang Lifu
AU  - Yao Jiatong
Y1  - 2022/03/24
PY  - 2022
N1  - https://doi.org/10.11648/j.ijfsb.20220701.15
DO  - 10.11648/j.ijfsb.20220701.15
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 16
EP  - 20
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20220701.15
AB  - Objective: To detec integron genes and the drug resistance of two foodborne salmonella from different sources in Jilin Province, explore the correlation between drug resistance and integron. Method: The minimum inhibitory concentration (MIC) method was used to detect drug resistance of 278 salmonella strains in Jilin Province. Real-time PCR was used to detect type I, II and III integron genes, and the correlation between drug resistance and integron gene carrier rate was compared. Result: The total drug resistance rate of 278 strains of food-borne Salmonella was 89.57% (249/278). The positive rates of type I integron, type II integron and type III integron were 51.44% (143/278), 4.32% (12/278) and 14.39% (40/278) respectively. The drug resistance rate of foodborne disease samples was 95.8% (207/216), type I integron was 58.8% (127/216), type II integron was 5.56% (12/216), and type III integron gene was 17.59% (38/216). The drug resistance rate of food (meat, egg) samples was 67.7% (42/62), type I integron was 25.81% (16/62), type III integron gene was 3.23% (2/62), and type II integron gene was detected. Conclusion: Through the surveillance of Salmonella drug resistance and Integron gene carrying rate in food safety risk surveillance and food-borne disease surveillance in Jilin Province, The gene carrying rate of type I and III Integron of Salmonella from food-borne diseases was significantly higher than that of food (meat and eggs). Integron gene system plays an important role in drug resistance transmission of foodborne pathogens. It is suggested that attention should be paid to the monitoring of salmonella drug resistance to ensure food safety and human health.
VL  - 7
IS  - 1
ER  -

Copy | Download