Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites

Olusola Adelowotan; Emmanuel Ayodele Oluyemi; Abiodun Odunlami Adegunwa

doi:doi:10.11648/j.ijema.20221005.12

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Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites

Olusola Adelowotan, Emmanuel Ayodele Oluyemi, Abiodun Odunlami Adegunwa

Published in International Journal of Environmental Monitoring and Analysis (Volume 10, Issue 5)

Received: 27 July 2022 Accepted: 19 August 2022 Published: 21 September 2022

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Abstract

Heavy metals pollution of the soil is of environmental concern and various methods have been applied to reduce its concentration. This study examines the accumulation of metal ion from aqueous solution and remediation potential of indigenous Escherichia intermedium and Sḫigella soṉnei isolated from polluted site. Standardized 2 mL of inoculum was added to 30 ppm of metal (Cu, Cr, Cd, Pb, and Ni) solution and was incubated at 37°C, the solution was centrifuged and cells were filtered off. The supernatant was analyzed with Atomic Absorption Spectrometer (AAS) for the determination of residual metal concentration. Total metal concentration in the polluted soil was extracted using aqua regia (HCl:HNO₃, 3:1) solution. Polluted soil solution was inoculated with 2 mL of standardized inoculum and was incubated at 37°C, the solution was subjected to gradient configuration and cells were filtered off. The resulting soil solution was subjected to acid digestion and analyzed with AAS for the determination of residual heavy metal concentration. Results from the bioaccumulation experiment show the absorbed concentration of metals from the aqueous solution by Escherichia intermedium as follows: 19.034 ± 0.26 mg/l (Cd), 5.799 ± 0.26 mg/l (Cr), 18.025 ± 0.17 mg/l (Cu), 9.930 ± 0.22 mg/l (Pb) and 11.179 ± 0.14 (Ni) as well as Sḫigella soṉnei with absorbed concentrations of 17.133 ± 0.17 mg/l (Cd), 8.937 ± 0.22mg/l (Cr), 17.686 ± 0.17 mg/l (Cu), 14.347 ± 0.02 mg/l (Pb), 10.776 ± 0.20mg/l (Ni). The range of percentage reduction of metal concentration in the ex-situ remediation of the four polluted soil sampled by Escherichia intermedium is as follows: 3.86-34.34% Cu, 18.06-50.39% Cr, 1.72-88.17% Cd, 2.89- 8.53% Pb, 19.31-44.03% Ni and Sḫigella soṉnei with 7.66-53.36% Cu, 17.39-50.11% Cr, 12.38%-ND Cd, 6.74-20.41% Pb and 44.17-53.13% Ni reduction. This study establishes that these isolates own good capability of accumulating and reducing metal ions from both aqueous solution and soil solution.

Published in	International Journal of Environmental Monitoring and Analysis (Volume 10, Issue 5)
DOI	10.11648/j.ijema.20221005.12
Page(s)	120-126
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Accumulation, Bacteria, Heavy Metals, Pollution, Remediation

References

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[2]	Bosecker, K. (2001). Microbial leaching in environmental clean-up programmes. Hydrometallurg, Vol. 59: 245-248.
[3]	Holt J. G., Kreig N. R., Sneath P. H. A., Staley J. T. and Williams S. T., (1994). Bergeys‘s manual of determination bacteriology. 9th ed., Lippincott Williams and wilkins, Baltimore, U.S.A., ISBN-10: 0683006037.
[4]	IR Spetrum Table and Chart (2020). Marck KGaA, Darmstadt. Germany www.sigmaaldrich.com
[5]	Ranasinghe P., Weerasinghe S., and Kaumal M. N., (2016). Determination of heavy metals in tilapia using various digestion methods. International Journal of Scientific Research and Innovative Technology. 3: 38-48.
[6]	Rui G., Yiming W., Yefei Z., Jin T. and Wei D. (2017). Cobalt (II) bioaccumulation and distribution in Rhodopseudomonas palustris. Biotechnology & Biotechnological Equipment, 31 (3): 527-534.
[7]	Sujitha D and Jayanthi M, (2014). Efficiency of immobilized microbial combination for the bioremediation of tannery effluents in Vellore District, Tamil Nadu, India. International Journal of Advanced Research in Biological Sciences 1 (8): 113–120.
[8]	Thalita F. A., Carlos A. S., Daniela A. A and Waldete J. O. C. (2014). Influence of digestion methods on the recovery of Iron, Zinc, Nickel, Chromium, Cadmium and Lead Contents in 11 organic residues. Revista Brasileira de Ciencia do Solo (RBCS). 38: 166-176.
[9]	Viggo Lindahl and Lars R. Bakken (1995). Evaluation of methods for extraction of bacteria from soil. FEMS Microbiology Ecology 16: 135-142.
[10]	Varghese R., Krishna M. P., Babu A. V. and Mohamed Hatha A. A (2012). Bioaccumulation of cadmium by Pseudomonas sp. isolated from metal polluted industrial region. Environmental Research, Engineering and Management, 61 (3), 58-64.

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Olusola Adelowotan, Emmanuel Ayodele Oluyemi, Abiodun Odunlami Adegunwa. (2022). Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites. International Journal of Environmental Monitoring and Analysis, 10(5), 120-126. https://doi.org/10.11648/j.ijema.20221005.12

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Olusola Adelowotan; Emmanuel Ayodele Oluyemi; Abiodun Odunlami Adegunwa. Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites. Int. J. Environ. Monit. Anal. 2022, 10(5), 120-126. doi: 10.11648/j.ijema.20221005.12

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Olusola Adelowotan, Emmanuel Ayodele Oluyemi, Abiodun Odunlami Adegunwa. Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites. Int J Environ Monit Anal. 2022;10(5):120-126. doi: 10.11648/j.ijema.20221005.12

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@article{10.11648/j.ijema.20221005.12,
  author = {Olusola Adelowotan and Emmanuel Ayodele Oluyemi and Abiodun Odunlami Adegunwa},
  title = {Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites},
  journal = {International Journal of Environmental Monitoring and Analysis},
  volume = {10},
  number = {5},
  pages = {120-126},
  doi = {10.11648/j.ijema.20221005.12},
  url = {https://doi.org/10.11648/j.ijema.20221005.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20221005.12},
  abstract = {Heavy metals pollution of the soil is of environmental concern and various methods have been applied to reduce its concentration. This study examines the accumulation of metal ion from aqueous solution and remediation potential of indigenous Escherichia intermedium and Sḫigella soṉnei isolated from polluted site. Standardized 2 mL of inoculum was added to 30 ppm of metal (Cu, Cr, Cd, Pb, and Ni) solution and was incubated at 37°C, the solution was centrifuged and cells were filtered off. The supernatant was analyzed with Atomic Absorption Spectrometer (AAS) for the determination of residual metal concentration. Total metal concentration in the polluted soil was extracted using aqua regia (HCl:HNO3, 3:1) solution. Polluted soil solution was inoculated with 2 mL of standardized inoculum and was incubated at 37°C, the solution was subjected to gradient configuration and cells were filtered off. The resulting soil solution was subjected to acid digestion and analyzed with AAS for the determination of residual heavy metal concentration. Results from the bioaccumulation experiment show the absorbed concentration of metals from the aqueous solution by Escherichia intermedium as follows: 19.034 ± 0.26 mg/l (Cd), 5.799 ± 0.26 mg/l (Cr), 18.025 ± 0.17 mg/l (Cu), 9.930 ± 0.22 mg/l (Pb) and 11.179 ± 0.14 (Ni) as well as Sḫigella soṉnei with absorbed concentrations of 17.133 ± 0.17 mg/l (Cd), 8.937 ± 0.22mg/l (Cr), 17.686 ± 0.17 mg/l (Cu), 14.347 ± 0.02 mg/l (Pb), 10.776 ± 0.20mg/l (Ni). The range of percentage reduction of metal concentration in the ex-situ remediation of the four polluted soil sampled by Escherichia intermedium is as follows: 3.86-34.34% Cu, 18.06-50.39% Cr, 1.72-88.17% Cd, 2.89- 8.53% Pb, 19.31-44.03% Ni and Sḫigella soṉnei with 7.66-53.36% Cu, 17.39-50.11% Cr, 12.38%-ND Cd, 6.74-20.41% Pb and 44.17-53.13% Ni reduction. This study establishes that these isolates own good capability of accumulating and reducing metal ions from both aqueous solution and soil solution.},
 year = {2022}
}

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TY  - JOUR
T1  - Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites
AU  - Olusola Adelowotan
AU  - Emmanuel Ayodele Oluyemi
AU  - Abiodun Odunlami Adegunwa
Y1  - 2022/09/21
PY  - 2022
N1  - https://doi.org/10.11648/j.ijema.20221005.12
DO  - 10.11648/j.ijema.20221005.12
T2  - International Journal of Environmental Monitoring and Analysis
JF  - International Journal of Environmental Monitoring and Analysis
JO  - International Journal of Environmental Monitoring and Analysis
SP  - 120
EP  - 126
PB  - Science Publishing Group
SN  - 2328-7667
UR  - https://doi.org/10.11648/j.ijema.20221005.12
AB  - Heavy metals pollution of the soil is of environmental concern and various methods have been applied to reduce its concentration. This study examines the accumulation of metal ion from aqueous solution and remediation potential of indigenous Escherichia intermedium and Sḫigella soṉnei isolated from polluted site. Standardized 2 mL of inoculum was added to 30 ppm of metal (Cu, Cr, Cd, Pb, and Ni) solution and was incubated at 37°C, the solution was centrifuged and cells were filtered off. The supernatant was analyzed with Atomic Absorption Spectrometer (AAS) for the determination of residual metal concentration. Total metal concentration in the polluted soil was extracted using aqua regia (HCl:HNO3, 3:1) solution. Polluted soil solution was inoculated with 2 mL of standardized inoculum and was incubated at 37°C, the solution was subjected to gradient configuration and cells were filtered off. The resulting soil solution was subjected to acid digestion and analyzed with AAS for the determination of residual heavy metal concentration. Results from the bioaccumulation experiment show the absorbed concentration of metals from the aqueous solution by Escherichia intermedium as follows: 19.034 ± 0.26 mg/l (Cd), 5.799 ± 0.26 mg/l (Cr), 18.025 ± 0.17 mg/l (Cu), 9.930 ± 0.22 mg/l (Pb) and 11.179 ± 0.14 (Ni) as well as Sḫigella soṉnei with absorbed concentrations of 17.133 ± 0.17 mg/l (Cd), 8.937 ± 0.22mg/l (Cr), 17.686 ± 0.17 mg/l (Cu), 14.347 ± 0.02 mg/l (Pb), 10.776 ± 0.20mg/l (Ni). The range of percentage reduction of metal concentration in the ex-situ remediation of the four polluted soil sampled by Escherichia intermedium is as follows: 3.86-34.34% Cu, 18.06-50.39% Cr, 1.72-88.17% Cd, 2.89- 8.53% Pb, 19.31-44.03% Ni and Sḫigella soṉnei with 7.66-53.36% Cu, 17.39-50.11% Cr, 12.38%-ND Cd, 6.74-20.41% Pb and 44.17-53.13% Ni reduction. This study establishes that these isolates own good capability of accumulating and reducing metal ions from both aqueous solution and soil solution.
VL  - 10
IS  - 5
ER  -

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Author Information

Olusola Adelowotan

Department of Chemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria
Emmanuel Ayodele Oluyemi

Department of Chemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria
Abiodun Odunlami Adegunwa

Department of Pure and Applied Chemistry, Osun State University, Osogbo, Nigeria

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Olusola Adelowotan, Emmanuel Ayodele Oluyemi, Abiodun Odunlami Adegunwa. (2022). Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites. International Journal of Environmental Monitoring and Analysis, 10(5), 120-126. https://doi.org/10.11648/j.ijema.20221005.12

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Olusola Adelowotan; Emmanuel Ayodele Oluyemi; Abiodun Odunlami Adegunwa. Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites. Int. J. Environ. Monit. Anal. 2022, 10(5), 120-126. doi: 10.11648/j.ijema.20221005.12

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AMA Style

Olusola Adelowotan, Emmanuel Ayodele Oluyemi, Abiodun Odunlami Adegunwa. Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites. Int J Environ Monit Anal. 2022;10(5):120-126. doi: 10.11648/j.ijema.20221005.12

Copy | Download

@article{10.11648/j.ijema.20221005.12,
  author = {Olusola Adelowotan and Emmanuel Ayodele Oluyemi and Abiodun Odunlami Adegunwa},
  title = {Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites},
  journal = {International Journal of Environmental Monitoring and Analysis},
  volume = {10},
  number = {5},
  pages = {120-126},
  doi = {10.11648/j.ijema.20221005.12},
  url = {https://doi.org/10.11648/j.ijema.20221005.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20221005.12},
  abstract = {Heavy metals pollution of the soil is of environmental concern and various methods have been applied to reduce its concentration. This study examines the accumulation of metal ion from aqueous solution and remediation potential of indigenous Escherichia intermedium and Sḫigella soṉnei isolated from polluted site. Standardized 2 mL of inoculum was added to 30 ppm of metal (Cu, Cr, Cd, Pb, and Ni) solution and was incubated at 37°C, the solution was centrifuged and cells were filtered off. The supernatant was analyzed with Atomic Absorption Spectrometer (AAS) for the determination of residual metal concentration. Total metal concentration in the polluted soil was extracted using aqua regia (HCl:HNO3, 3:1) solution. Polluted soil solution was inoculated with 2 mL of standardized inoculum and was incubated at 37°C, the solution was subjected to gradient configuration and cells were filtered off. The resulting soil solution was subjected to acid digestion and analyzed with AAS for the determination of residual heavy metal concentration. Results from the bioaccumulation experiment show the absorbed concentration of metals from the aqueous solution by Escherichia intermedium as follows: 19.034 ± 0.26 mg/l (Cd), 5.799 ± 0.26 mg/l (Cr), 18.025 ± 0.17 mg/l (Cu), 9.930 ± 0.22 mg/l (Pb) and 11.179 ± 0.14 (Ni) as well as Sḫigella soṉnei with absorbed concentrations of 17.133 ± 0.17 mg/l (Cd), 8.937 ± 0.22mg/l (Cr), 17.686 ± 0.17 mg/l (Cu), 14.347 ± 0.02 mg/l (Pb), 10.776 ± 0.20mg/l (Ni). The range of percentage reduction of metal concentration in the ex-situ remediation of the four polluted soil sampled by Escherichia intermedium is as follows: 3.86-34.34% Cu, 18.06-50.39% Cr, 1.72-88.17% Cd, 2.89- 8.53% Pb, 19.31-44.03% Ni and Sḫigella soṉnei with 7.66-53.36% Cu, 17.39-50.11% Cr, 12.38%-ND Cd, 6.74-20.41% Pb and 44.17-53.13% Ni reduction. This study establishes that these isolates own good capability of accumulating and reducing metal ions from both aqueous solution and soil solution.},
 year = {2022}
}

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TY  - JOUR
T1  - Accumulation of Heavy Metal ion and Ex-situ Remediation of Polluted Soil Using Escherichia intermedium and Shigella sonnei Isolated from Polluted Sites
AU  - Olusola Adelowotan
AU  - Emmanuel Ayodele Oluyemi
AU  - Abiodun Odunlami Adegunwa
Y1  - 2022/09/21
PY  - 2022
N1  - https://doi.org/10.11648/j.ijema.20221005.12
DO  - 10.11648/j.ijema.20221005.12
T2  - International Journal of Environmental Monitoring and Analysis
JF  - International Journal of Environmental Monitoring and Analysis
JO  - International Journal of Environmental Monitoring and Analysis
SP  - 120
EP  - 126
PB  - Science Publishing Group
SN  - 2328-7667
UR  - https://doi.org/10.11648/j.ijema.20221005.12
AB  - Heavy metals pollution of the soil is of environmental concern and various methods have been applied to reduce its concentration. This study examines the accumulation of metal ion from aqueous solution and remediation potential of indigenous Escherichia intermedium and Sḫigella soṉnei isolated from polluted site. Standardized 2 mL of inoculum was added to 30 ppm of metal (Cu, Cr, Cd, Pb, and Ni) solution and was incubated at 37°C, the solution was centrifuged and cells were filtered off. The supernatant was analyzed with Atomic Absorption Spectrometer (AAS) for the determination of residual metal concentration. Total metal concentration in the polluted soil was extracted using aqua regia (HCl:HNO3, 3:1) solution. Polluted soil solution was inoculated with 2 mL of standardized inoculum and was incubated at 37°C, the solution was subjected to gradient configuration and cells were filtered off. The resulting soil solution was subjected to acid digestion and analyzed with AAS for the determination of residual heavy metal concentration. Results from the bioaccumulation experiment show the absorbed concentration of metals from the aqueous solution by Escherichia intermedium as follows: 19.034 ± 0.26 mg/l (Cd), 5.799 ± 0.26 mg/l (Cr), 18.025 ± 0.17 mg/l (Cu), 9.930 ± 0.22 mg/l (Pb) and 11.179 ± 0.14 (Ni) as well as Sḫigella soṉnei with absorbed concentrations of 17.133 ± 0.17 mg/l (Cd), 8.937 ± 0.22mg/l (Cr), 17.686 ± 0.17 mg/l (Cu), 14.347 ± 0.02 mg/l (Pb), 10.776 ± 0.20mg/l (Ni). The range of percentage reduction of metal concentration in the ex-situ remediation of the four polluted soil sampled by Escherichia intermedium is as follows: 3.86-34.34% Cu, 18.06-50.39% Cr, 1.72-88.17% Cd, 2.89- 8.53% Pb, 19.31-44.03% Ni and Sḫigella soṉnei with 7.66-53.36% Cu, 17.39-50.11% Cr, 12.38%-ND Cd, 6.74-20.41% Pb and 44.17-53.13% Ni reduction. This study establishes that these isolates own good capability of accumulating and reducing metal ions from both aqueous solution and soil solution.
VL  - 10
IS  - 5
ER  -

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