Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice

Jeweldai Vedekoi; Sokeng Dongmo Selestin; Kamtchouing Pierre

doi:doi:10.11648/j.ijcems.20210703.11

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Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice

Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre

Published in International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 3)

Received: 1 March 2021 Accepted: 12 May 2021 Published: 27 May 2021

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Abstract

Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.

Published in	International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 3)
DOI	10.11648/j.ijcems.20210703.11
Page(s)	55-63
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Boswellia Dalzielii, Inflammation, Anti-inflammatory, Anti-nociceptive, Phytochemicals, Mice

References

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Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre. (2021). Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. International Journal of Clinical and Experimental Medical Sciences, 7(3), 55-63. https://doi.org/10.11648/j.ijcems.20210703.11

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ACS Style

Jeweldai Vedekoi; Sokeng Dongmo Selestin; Kamtchouing Pierre. Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. Int. J. Clin. Exp. Med. Sci. 2021, 7(3), 55-63. doi: 10.11648/j.ijcems.20210703.11

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AMA Style

Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre. Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. Int J Clin Exp Med Sci. 2021;7(3):55-63. doi: 10.11648/j.ijcems.20210703.11

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@article{10.11648/j.ijcems.20210703.11,
  author = {Jeweldai Vedekoi and Sokeng Dongmo Selestin and Kamtchouing Pierre},
  title = {Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {3},
  pages = {55-63},
  doi = {10.11648/j.ijcems.20210703.11},
  url = {https://doi.org/10.11648/j.ijcems.20210703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210703.11},
  abstract = {Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.},
 year = {2021}
}

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TY  - JOUR
T1  - Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice
AU  - Jeweldai Vedekoi
AU  - Sokeng Dongmo Selestin
AU  - Kamtchouing Pierre
Y1  - 2021/05/27
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcems.20210703.11
DO  - 10.11648/j.ijcems.20210703.11
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 55
EP  - 63
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20210703.11
AB  - Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.
VL  - 7
IS  - 3
ER  -

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Author Information

Jeweldai Vedekoi

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Sokeng Dongmo Selestin

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Kamtchouing Pierre

Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

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Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre. (2021). Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. International Journal of Clinical and Experimental Medical Sciences, 7(3), 55-63. https://doi.org/10.11648/j.ijcems.20210703.11

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ACS Style

Jeweldai Vedekoi; Sokeng Dongmo Selestin; Kamtchouing Pierre. Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. Int. J. Clin. Exp. Med. Sci. 2021, 7(3), 55-63. doi: 10.11648/j.ijcems.20210703.11

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AMA Style

Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre. Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. Int J Clin Exp Med Sci. 2021;7(3):55-63. doi: 10.11648/j.ijcems.20210703.11

Copy | Download

@article{10.11648/j.ijcems.20210703.11,
  author = {Jeweldai Vedekoi and Sokeng Dongmo Selestin and Kamtchouing Pierre},
  title = {Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {3},
  pages = {55-63},
  doi = {10.11648/j.ijcems.20210703.11},
  url = {https://doi.org/10.11648/j.ijcems.20210703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210703.11},
  abstract = {Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice
AU  - Jeweldai Vedekoi
AU  - Sokeng Dongmo Selestin
AU  - Kamtchouing Pierre
Y1  - 2021/05/27
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcems.20210703.11
DO  - 10.11648/j.ijcems.20210703.11
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 55
EP  - 63
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20210703.11
AB  - Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.
VL  - 7
IS  - 3
ER  -

Copy | Download