The main objective of this work was the design and construction of a bio-based novel photovoltaic. For this purpose, two prototypes were designed and constructed. The first was an efficient extraction prototype of active pigments, and then the validation of the pureness of the extracted pigments took place by using the Ultra Violet Visible region (UV-V) spectra and Infra-Red (IR) spectrum. And, the second prototype as novel bio-photovoltaic hydrogen cell (NB-PV) was consisting of three glass sections donor, acceptor and membrane. These sections were containing a certain water type dissolved active pigments extracted from spinach (Spinacia Oleracea) by using the first prototype. So, both water dissolved chlorophyll a (Chl a) and chlorophyll b (Chl b), and the water dissolved pheophytin (pheo) were contained in the donor and acceptor sections respectively. In addition, the third section enclosed a precipitated ferredoxin (Fd) on rice straw as membrane. The membrane design allowed only electron transportation from donor section to the acceptor section. This second prototype was used also for water dissociation (2H+ and O-) as byproducts of such novel cell. Hence, the performance of the novel cell was determined based on measuring the average output voltage and lifetime of such cell. The experimental program was designed to investigate different variables that affect the performance of the present cell such as operating pressure, water type and light intensity. The results showed that the average voltage output flux diminishes with any slight increase of the operating pressure above the atmospheric one. The maximum values of the output flux were achieved when the active pigments were dissolved in sea water (SW). The results also, indicated that the light intensity had a minimal effect on the average voltage output flux.
| Published in | Engineering and Applied Sciences (Volume 1, Issue 4) |
| DOI | 10.11648/j.eas.20160104.13 |
| Page(s) | 87-98 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Chlorophylls, Pheophytin, Ferredoxin, UV-V Spectra, IR Spectrum, Bio-Photovoltaic, Hydrogen, Photosynthesis
| [1] | Gray HB, "Solar Fuel", Engineering and Science No. 3, 1997. |
| [2] | http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookPS.html, 2005. |
| [3] | Zouni A, Witt H, and Kern J, "Crystal Structure of Photosystem II from Synechococcuselongatus at 3.8 A resolution", Nature, Vol. 409, pp. 739-772, 2001. |
| [4] | Germano M, Shkuropatov AY, and Permenttier H, "Pigment Organisation and their Interactions in Reaction Centers of Photosystem II", Biochemistry, Vol. 40, pp. 114-196, 2001. |
| [5] | Barber J, and Archer MD, "P680 the primary electron donor of Photosystem II", J. Photoch. Photobio. A., Vol. 42, pp. 97-106, 2001. |
| [6] | Andrizhiyevskaya EG, Chojnicka A, Diner JA, Grondelle R, and Dekker JP, "Origin of the F685 and F695 Fluorescence in Photosystem II", Photosynthesis Research, Springer, Vol. 84, pp. 173-180, 2005. |
| [7] | Furukawa Y, Moriuchi T, and Morishima K, “Direct Photo Synthetic/Metabolic Bio-Fuel Cells (Dpbfc) ", power MEMS 2005 Conference Paper No 184-8588 Japan. |
| [8] | Osman ZA, Elsanousi SM, and Elsheikh E, "Extraction of ferredoxin from spinach leaves and its effect on Clostridium perfringens (anaerobe) growth”, European Journal of Experimental Biology, 3 (4), pp. 267-285, 2013. |
| [9] | Hosikian A, Lim S, Halim R, and Danquah MK, "Chlorophyll Extraction from Microalgae: A Review on the Process Engineering Aspects", Review Article, International Journal of Chemical Engineering, Hindawi Publishing Corporation, Vol. 2010, Article ID 391632, 2010. |
| [10] | Abdelrazek EM, El Damrawi G, Elashmawi IS, and El-Shahawy A, "The influence of g-irradiation on some physical properties of chlorophyll/PMMA films", Applied Surface Science, Elsevier, Vol. 256, pp. 2711–2718, 2010. |
| [11] | Würfel P, "Solar Cell Operational Principles", Physics of Solar Cells: From Principles to New Concepts, Wiley-VCH, Weinheim, Ch.4. p 4.1., 2005. |
| [12] | Yang C, Chang K, Yin M, and Huang H, "Methods for Determination of the Chlorophylls and their Derivatives", Taiwania, Vol. 43 (2) pp. 116-122, 1988. |
| [13] | Dere Ș, Geuneș T and Sivaci R, "Spectrophotometric Determination of Chlorophyll A, B and Total Carotenoid Content of Some Algae Species Using Different Solvents", Tr. J. of Botany, Vol. 22 pp. 13-17, 1998. |
| [14] | Anderson AFH, "Extraction and Purification of Chlorophyll", Patent No. 3,258,467 USPO, June, 28, 1966. |
| [15] | McLaughlin R, Masters K, William G, Hao T, and Robert L, "Extraction and Thin-Layer Chromatography of Chlorophyll A and B from Spinach", J. Chem. Ed., Vol. 81, pp. 385-387, 2004. |
| [16] | Dikio ED and Isabirye DA, "Short Communication: Isolation of Chlorophyll a from Spinach Leaves", Bull. Chem. Soc. Ethiop., ISSN 1011-3924, Vol. 22 (2), pp. 301-304, 2008. |
| [17] | Murata N, Araki S, Fujita Y, Suzuki K, Kuwabara T, and Mathis P, "Stoichiometric determination of pheophytin in photosystem II of oxygenic photosynthesis", Martinus Ni]hoff/Dr. W. Junk Publishers, Dordrecht, Photosynthesis Research, Vol. 9, pp. 63-70, 1986. |
| [18] | Hornero-Mendez D, and Minguez-Mosquera MI, "Spectra Determination of Chlorophylls and Carotenoids", J. Agric. Food Chem., Vol. 48, pp. 1617-1623, 2000. |
| [19] | Watanabe T, Hongu A, and Honda K, "Separation of Chlorophylls", Anal. Chem., Vol. 56, pp. 251-259, 1984. |
| [20] | Jeffrey SW, "Chromatographic Separation of Chlorophylls and Carotenoids from Marine Algae", Biochem. J., Vol. 80, pp. 336-342, 1961. |
| [21] | Schmid VHR, Thome P, Reuhle W, Paulsen H, Keuhlbrandt W, and Rog H, "Chlorophyll b is involved in long-wavelength spectral properties of light-harvesting complexes LHC I and LHC II", FEBS 24932, FEBS Letters Vol. 499, pp. 27-31, 2001. |
| [22] | Goedheer JC, Vernon LP, and Seeley GR, "The Chlorophylls", Academic Press, NY, pp. 143-184, 1966. |
| [23] | Klimov VV, Klevanik AV, and Shuvalov VA, "Reduction of Pheophytin in the Primary Light Reaction of Photosystem", FEBS LETTERS, Volume 82, number 2, pp. 183-186, October 1977. |
| [24] | King LL, "Chlorophyll Diagenesis in the Water Column and Sediments of the Black Sea", PhD dissertation at MIT and WHOI, pp. 45-90, 1992. |
| [25] | Ramamurthy N, and Kannan S, "Fourier Transform Infrared Spectroscopic Analysis of a Plant (CALOTROPIS GIGANTEA Linn) from an Industrial Village, Cuddalore Dt, Tamilnadu, INDIA", ROMANIAN J. BIOPHYS., Vol. 17, No. 4, P. 269–276, BUCHAREST, 2007. |
| [26] | Holt AS, and Jacobs EE, "Infra-Red Absorption Spectra of Chlorophylls and Dervatives", PLANT PHYSIOLOGY, pp. 553-559, 1955. |
| [27] | Goodwin TW, "Chemistry and Biochemistry of Plant Pigments", Vol. 2, 2nd ed., Academic Press: London; pp 1-32, 1976. |
| [28] | Boucher LJ, Strain HH, and Katz JJ, "Infrared Spectra of Plant Pigments", J. Am. Chem. Soc., 88, 1341, 1966. |
| [29] | Boucher LJ and Katz JJ, "Infrared of chlorophylls", J. Am. Chem. Soc., 89, 1340, 1966. |
| [30] | Ballschmiter K, and Katz JJ, "An infrared study of chlorophyll-chlorophyll and chlorophyll-water interactions", J. Am. Chem. Soc., 91, 2661, 1969. |
| [31] | Rao CNR, "Chemical applications of infrared spectroscopy", Academic Press, New York and London, 1963. |
| [32] | Bellamy LJ, "Infrared spectra of complex molecules", Chapman Hall, London, 1975. |
APA Style
Osayed S. M. Abu-Elyazeed, Mohamed A. El-Sayed, Youssef A. Attai, Mohamed A. Nawar, Hatem M. Sadek. (2017). On the Performance of a Novel Bio-photovoltaic Hydrogen Cell from Green Leaves. Engineering and Applied Sciences, 1(4), 87-98. https://doi.org/10.11648/j.eas.20160104.13
ACS Style
Osayed S. M. Abu-Elyazeed; Mohamed A. El-Sayed; Youssef A. Attai; Mohamed A. Nawar; Hatem M. Sadek. On the Performance of a Novel Bio-photovoltaic Hydrogen Cell from Green Leaves. Eng. Appl. Sci. 2017, 1(4), 87-98. doi: 10.11648/j.eas.20160104.13
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.eas.20160104.13,
author = {Osayed S. M. Abu-Elyazeed and Mohamed A. El-Sayed and Youssef A. Attai and Mohamed A. Nawar and Hatem M. Sadek},
title = {On the Performance of a Novel Bio-photovoltaic Hydrogen Cell from Green Leaves},
journal = {Engineering and Applied Sciences},
volume = {1},
number = {4},
pages = {87-98},
doi = {10.11648/j.eas.20160104.13},
url = {https://doi.org/10.11648/j.eas.20160104.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.eas.20160104.13},
abstract = {The main objective of this work was the design and construction of a bio-based novel photovoltaic. For this purpose, two prototypes were designed and constructed. The first was an efficient extraction prototype of active pigments, and then the validation of the pureness of the extracted pigments took place by using the Ultra Violet Visible region (UV-V) spectra and Infra-Red (IR) spectrum. And, the second prototype as novel bio-photovoltaic hydrogen cell (NB-PV) was consisting of three glass sections donor, acceptor and membrane. These sections were containing a certain water type dissolved active pigments extracted from spinach (Spinacia Oleracea) by using the first prototype. So, both water dissolved chlorophyll a (Chl a) and chlorophyll b (Chl b), and the water dissolved pheophytin (pheo) were contained in the donor and acceptor sections respectively. In addition, the third section enclosed a precipitated ferredoxin (Fd) on rice straw as membrane. The membrane design allowed only electron transportation from donor section to the acceptor section. This second prototype was used also for water dissociation (2H+ and O-) as byproducts of such novel cell. Hence, the performance of the novel cell was determined based on measuring the average output voltage and lifetime of such cell. The experimental program was designed to investigate different variables that affect the performance of the present cell such as operating pressure, water type and light intensity. The results showed that the average voltage output flux diminishes with any slight increase of the operating pressure above the atmospheric one. The maximum values of the output flux were achieved when the active pigments were dissolved in sea water (SW). The results also, indicated that the light intensity had a minimal effect on the average voltage output flux.},
year = {2017}
}
TY - JOUR T1 - On the Performance of a Novel Bio-photovoltaic Hydrogen Cell from Green Leaves AU - Osayed S. M. Abu-Elyazeed AU - Mohamed A. El-Sayed AU - Youssef A. Attai AU - Mohamed A. Nawar AU - Hatem M. Sadek Y1 - 2017/01/23 PY - 2017 N1 - https://doi.org/10.11648/j.eas.20160104.13 DO - 10.11648/j.eas.20160104.13 T2 - Engineering and Applied Sciences JF - Engineering and Applied Sciences JO - Engineering and Applied Sciences SP - 87 EP - 98 PB - Science Publishing Group SN - 2575-1468 UR - https://doi.org/10.11648/j.eas.20160104.13 AB - The main objective of this work was the design and construction of a bio-based novel photovoltaic. For this purpose, two prototypes were designed and constructed. The first was an efficient extraction prototype of active pigments, and then the validation of the pureness of the extracted pigments took place by using the Ultra Violet Visible region (UV-V) spectra and Infra-Red (IR) spectrum. And, the second prototype as novel bio-photovoltaic hydrogen cell (NB-PV) was consisting of three glass sections donor, acceptor and membrane. These sections were containing a certain water type dissolved active pigments extracted from spinach (Spinacia Oleracea) by using the first prototype. So, both water dissolved chlorophyll a (Chl a) and chlorophyll b (Chl b), and the water dissolved pheophytin (pheo) were contained in the donor and acceptor sections respectively. In addition, the third section enclosed a precipitated ferredoxin (Fd) on rice straw as membrane. The membrane design allowed only electron transportation from donor section to the acceptor section. This second prototype was used also for water dissociation (2H+ and O-) as byproducts of such novel cell. Hence, the performance of the novel cell was determined based on measuring the average output voltage and lifetime of such cell. The experimental program was designed to investigate different variables that affect the performance of the present cell such as operating pressure, water type and light intensity. The results showed that the average voltage output flux diminishes with any slight increase of the operating pressure above the atmospheric one. The maximum values of the output flux were achieved when the active pigments were dissolved in sea water (SW). The results also, indicated that the light intensity had a minimal effect on the average voltage output flux. VL - 1 IS - 4 ER -
Information
Email: