Background: Homocysteine is an intermediate sulfur amino acid of methionine metabolism. Hyperhomocysteinemia, characterized by increased level of homocysteine, is an independent and modifiable vascular risk factor which metabolic pathway involves vitamins B6, folate and vitamin B12. Objective: We compared the prevalence of MTHFRC677T polymorphism, homocysteine folate and vitamin B12 in ischemic stroke patient’s subgroups. Methods: We conducted a cross-sectional analytical study. The study included 128 consecutive ischemic stroke patients associated with hyperhomocysteinemia. The MTHFRC677T polymorphism was investigated by TaqMan probes (thermos Fisher Scientific) combined with polymerase chain reaction (PCR). We compared the prevalence of MTHFRC677T polymorphism and homocysteine level in ischemic stroke patient’s subgroups. We adjusted the variable homocysteine level to the covariates, MTHFR polymorphism, folate and vitamin B12 with ANCOVA. Results: The sex ratio (men/women) was 1.5 with an average age of 60 years. The prevalence of MTHFRC677T polymorphism was 19.5% with 18% CT and 1.5% TT. Homocysteine level was 29.89 µmol/l in wildtype patients, 26.54 µmol/l in patients with CT genotype, and 56.17 µmol/l in patients with TT genotype (t=2.04, p=0.033, CI 95% [0.017; 0.407]). The MTHFR polymorphism prevalence, homocysteine, folate and vitamin B12 level did not differ between large brain infarction and multiple small brain infarction patients respectively (chi square: Qobs=0.05, p=0.94, 95% CI; ttest: t=0.716, df=126, p=0.475, 95% CI). Conclusion: The MTHFRT677T genotype increases homocysteine level. MTHFR polymorphism and homocysteine did not influence the subtypes of brain ischemic stroke.
| Published in | Clinical Neurology and Neuroscience (Volume 5, Issue 3) |
| DOI | 10.11648/j.cnn.20210503.14 |
| Page(s) | 55-59 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
MTHFRC677T Polymorphism, Homocysteine, Ischemic Stroke
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APA Style
Damelan Kombate, Sirui Zhou, Panabalo Waklatsi, David Ksc Ahanogbe, Komi Assogba, et al. (2021). MTHFRC677T Polymorphism and Hyperhomocysteinemia in Ischemic Stroke Patients. Clinical Neurology and Neuroscience, 5(3), 55-59. https://doi.org/10.11648/j.cnn.20210503.14
ACS Style
Damelan Kombate; Sirui Zhou; Panabalo Waklatsi; David Ksc Ahanogbe; Komi Assogba, et al. MTHFRC677T Polymorphism and Hyperhomocysteinemia in Ischemic Stroke Patients. Clin. Neurol. Neurosci. 2021, 5(3), 55-59. doi: 10.11648/j.cnn.20210503.14
AMA Style
Damelan Kombate, Sirui Zhou, Panabalo Waklatsi, David Ksc Ahanogbe, Komi Assogba, et al. MTHFRC677T Polymorphism and Hyperhomocysteinemia in Ischemic Stroke Patients. Clin Neurol Neurosci. 2021;5(3):55-59. doi: 10.11648/j.cnn.20210503.14
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Download@article{10.11648/j.cnn.20210503.14,
author = {Damelan Kombate and Sirui Zhou and Panabalo Waklatsi and David Ksc Ahanogbe and Komi Assogba and Emile Kou’santa Amouzou and Agnon Ayélola Koffi Balogou and Guy Armand Rouleau},
title = {MTHFRC677T Polymorphism and Hyperhomocysteinemia in Ischemic Stroke Patients},
journal = {Clinical Neurology and Neuroscience},
volume = {5},
number = {3},
pages = {55-59},
doi = {10.11648/j.cnn.20210503.14},
url = {https://doi.org/10.11648/j.cnn.20210503.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cnn.20210503.14},
abstract = {Background: Homocysteine is an intermediate sulfur amino acid of methionine metabolism. Hyperhomocysteinemia, characterized by increased level of homocysteine, is an independent and modifiable vascular risk factor which metabolic pathway involves vitamins B6, folate and vitamin B12. Objective: We compared the prevalence of MTHFRC677T polymorphism, homocysteine folate and vitamin B12 in ischemic stroke patient’s subgroups. Methods: We conducted a cross-sectional analytical study. The study included 128 consecutive ischemic stroke patients associated with hyperhomocysteinemia. The MTHFRC677T polymorphism was investigated by TaqMan probes (thermos Fisher Scientific) combined with polymerase chain reaction (PCR). We compared the prevalence of MTHFRC677T polymorphism and homocysteine level in ischemic stroke patient’s subgroups. We adjusted the variable homocysteine level to the covariates, MTHFR polymorphism, folate and vitamin B12 with ANCOVA. Results: The sex ratio (men/women) was 1.5 with an average age of 60 years. The prevalence of MTHFRC677T polymorphism was 19.5% with 18% CT and 1.5% TT. Homocysteine level was 29.89 µmol/l in wildtype patients, 26.54 µmol/l in patients with CT genotype, and 56.17 µmol/l in patients with TT genotype (t=2.04, p=0.033, CI 95% [0.017; 0.407]). The MTHFR polymorphism prevalence, homocysteine, folate and vitamin B12 level did not differ between large brain infarction and multiple small brain infarction patients respectively (chi square: Qobs=0.05, p=0.94, 95% CI; ttest: t=0.716, df=126, p=0.475, 95% CI). Conclusion: The MTHFRT677T genotype increases homocysteine level. MTHFR polymorphism and homocysteine did not influence the subtypes of brain ischemic stroke.},
year = {2021}
}
TY - JOUR T1 - MTHFRC677T Polymorphism and Hyperhomocysteinemia in Ischemic Stroke Patients AU - Damelan Kombate AU - Sirui Zhou AU - Panabalo Waklatsi AU - David Ksc Ahanogbe AU - Komi Assogba AU - Emile Kou’santa Amouzou AU - Agnon Ayélola Koffi Balogou AU - Guy Armand Rouleau Y1 - 2021/08/18 PY - 2021 N1 - https://doi.org/10.11648/j.cnn.20210503.14 DO - 10.11648/j.cnn.20210503.14 T2 - Clinical Neurology and Neuroscience JF - Clinical Neurology and Neuroscience JO - Clinical Neurology and Neuroscience SP - 55 EP - 59 PB - Science Publishing Group SN - 2578-8930 UR - https://doi.org/10.11648/j.cnn.20210503.14 AB - Background: Homocysteine is an intermediate sulfur amino acid of methionine metabolism. Hyperhomocysteinemia, characterized by increased level of homocysteine, is an independent and modifiable vascular risk factor which metabolic pathway involves vitamins B6, folate and vitamin B12. Objective: We compared the prevalence of MTHFRC677T polymorphism, homocysteine folate and vitamin B12 in ischemic stroke patient’s subgroups. Methods: We conducted a cross-sectional analytical study. The study included 128 consecutive ischemic stroke patients associated with hyperhomocysteinemia. The MTHFRC677T polymorphism was investigated by TaqMan probes (thermos Fisher Scientific) combined with polymerase chain reaction (PCR). We compared the prevalence of MTHFRC677T polymorphism and homocysteine level in ischemic stroke patient’s subgroups. We adjusted the variable homocysteine level to the covariates, MTHFR polymorphism, folate and vitamin B12 with ANCOVA. Results: The sex ratio (men/women) was 1.5 with an average age of 60 years. The prevalence of MTHFRC677T polymorphism was 19.5% with 18% CT and 1.5% TT. Homocysteine level was 29.89 µmol/l in wildtype patients, 26.54 µmol/l in patients with CT genotype, and 56.17 µmol/l in patients with TT genotype (t=2.04, p=0.033, CI 95% [0.017; 0.407]). The MTHFR polymorphism prevalence, homocysteine, folate and vitamin B12 level did not differ between large brain infarction and multiple small brain infarction patients respectively (chi square: Qobs=0.05, p=0.94, 95% CI; ttest: t=0.716, df=126, p=0.475, 95% CI). Conclusion: The MTHFRT677T genotype increases homocysteine level. MTHFR polymorphism and homocysteine did not influence the subtypes of brain ischemic stroke. VL - 5 IS - 3 ER -
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