Atopy may be defined as the production of abnormal amounts of Immunoglobulin E (IgE) antibodies in response to aeroallergens, assessed by positive skin-prick test (SPT) responses. Allergic asthma is the result of a complex multifactorial interplay between genes and the environment. The interaction of genetic and environmental factors may increase the complexity associated with allergic asthma. We investigated the degree of association of skin reactivity and their association with seven polymorphisms in IL-4, IL-5, IL-13, and IL-9 genes on chromosome 5q31-q33 region, and three polymorphisms in the IL-4Rα gene on chromosome 16 p contributing to allergic asthma in a population-based sample of Sudanese families. 70 families, including (150 cases and 140 controls), were recruited. Asthmatic status was confirmed by pulmonary function tests (PFTs); a SPT was done using 14 aeroallergens. Genotyping for 10 single-nucleotide polymorphisms (SNPs); one SNP in the IL-4 gene; one SNP in the IL-5 gene; two SNPs in the IL-13 gene; three SNPs in the IL-9 gene, three SNPs in the IL-4Rα gene were obtained by using multiplex PCR Mass ARRAY matrix MALDI-TOF mass spectrometry;. For the SPT reactivity, out of the 14 allergens, seven showed a significant hypersensitivity in the majority of asthmatics, including; house dust mites (HDM), mixed moulds, D. Peterenyssinus (DP), mixed ragweed, grass pollens, and cat hair. The strongest sensitizer was HDM (P = 0.001). The analysis of SNPs for association with SPT reactivity revealed the following; the SNPs in IL-13 in addition, IL-4Rα showed evidence association with SPT reactivity symptoms, but SNPs in the IL-4 gene associated with marginally significant (P = 0.05). An interaction of (A−646G) allele of IL-13 with minor alleles of IL-5, IL-9, and IL-4Rα genes, showed a significant association with reactivity SPT. Asthmatics showed hypersensitivity to common aeroallergens, the more prevalent allergen was mixed moulds; the most significant one was HDM. More reactive alleles associated with skin reactivity were; (A-G) of the IL-13 gene and (G-A) of the IL-4R gene. An interaction of the IL-13 gene with IL-5, IL-9, and IL-4Rα genes, have a significant association with reactivity SPT. There is a correlation between the IL-13 gene, atopy, and asthma in Sudanese.
| Published in | Biochemistry and Molecular Biology (Volume 8, Issue 2) |
| DOI | 10.11648/j.bmb.20230802.11 |
| Page(s) | 21-28 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Allergic Asthma, Allergens, Skin-Prick Hypersensitivity, Interleukin, Gene, Polymorphism, 5q31-q33, 16p11
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APA Style
Amel Osman Gundi, Fatima Omer Hamed, Maha Hassan Agraa, Hiba Salaheldin Mohamed. (2023). Reactivity Skin-Prick Test with Common Allergens and Their Genetic Association with Polymorphisms in IL-9, IL-13, IL-4, IL-5 & IL-4Rα Genes among Asthmatics in Sudan. Biochemistry and Molecular Biology, 8(2), 21-28. https://doi.org/10.11648/j.bmb.20230802.11
ACS Style
Amel Osman Gundi; Fatima Omer Hamed; Maha Hassan Agraa; Hiba Salaheldin Mohamed. Reactivity Skin-Prick Test with Common Allergens and Their Genetic Association with Polymorphisms in IL-9, IL-13, IL-4, IL-5 & IL-4Rα Genes among Asthmatics in Sudan. Biochem. Mol. Biol. 2023, 8(2), 21-28. doi: 10.11648/j.bmb.20230802.11
AMA Style
Amel Osman Gundi, Fatima Omer Hamed, Maha Hassan Agraa, Hiba Salaheldin Mohamed. Reactivity Skin-Prick Test with Common Allergens and Their Genetic Association with Polymorphisms in IL-9, IL-13, IL-4, IL-5 & IL-4Rα Genes among Asthmatics in Sudan. Biochem Mol Biol. 2023;8(2):21-28. doi: 10.11648/j.bmb.20230802.11
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Download@article{10.11648/j.bmb.20230802.11,
author = {Amel Osman Gundi and Fatima Omer Hamed and Maha Hassan Agraa and Hiba Salaheldin Mohamed},
title = {Reactivity Skin-Prick Test with Common Allergens and Their Genetic Association with Polymorphisms in IL-9, IL-13, IL-4, IL-5 & IL-4Rα Genes among Asthmatics in Sudan},
journal = {Biochemistry and Molecular Biology},
volume = {8},
number = {2},
pages = {21-28},
doi = {10.11648/j.bmb.20230802.11},
url = {https://doi.org/10.11648/j.bmb.20230802.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20230802.11},
abstract = {Atopy may be defined as the production of abnormal amounts of Immunoglobulin E (IgE) antibodies in response to aeroallergens, assessed by positive skin-prick test (SPT) responses. Allergic asthma is the result of a complex multifactorial interplay between genes and the environment. The interaction of genetic and environmental factors may increase the complexity associated with allergic asthma. We investigated the degree of association of skin reactivity and their association with seven polymorphisms in IL-4, IL-5, IL-13, and IL-9 genes on chromosome 5q31-q33 region, and three polymorphisms in the IL-4Rα gene on chromosome 16 p contributing to allergic asthma in a population-based sample of Sudanese families. 70 families, including (150 cases and 140 controls), were recruited. Asthmatic status was confirmed by pulmonary function tests (PFTs); a SPT was done using 14 aeroallergens. Genotyping for 10 single-nucleotide polymorphisms (SNPs); one SNP in the IL-4 gene; one SNP in the IL-5 gene; two SNPs in the IL-13 gene; three SNPs in the IL-9 gene, three SNPs in the IL-4Rα gene were obtained by using multiplex PCR Mass ARRAY matrix MALDI-TOF mass spectrometry;. For the SPT reactivity, out of the 14 allergens, seven showed a significant hypersensitivity in the majority of asthmatics, including; house dust mites (HDM), mixed moulds, D. Peterenyssinus (DP), mixed ragweed, grass pollens, and cat hair. The strongest sensitizer was HDM (P = 0.001). The analysis of SNPs for association with SPT reactivity revealed the following; the SNPs in IL-13 in addition, IL-4Rα showed evidence association with SPT reactivity symptoms, but SNPs in the IL-4 gene associated with marginally significant (P = 0.05). An interaction of (A−646G) allele of IL-13 with minor alleles of IL-5, IL-9, and IL-4Rα genes, showed a significant association with reactivity SPT. Asthmatics showed hypersensitivity to common aeroallergens, the more prevalent allergen was mixed moulds; the most significant one was HDM. More reactive alleles associated with skin reactivity were; (A-G) of the IL-13 gene and (G-A) of the IL-4R gene. An interaction of the IL-13 gene with IL-5, IL-9, and IL-4Rα genes, have a significant association with reactivity SPT. There is a correlation between the IL-13 gene, atopy, and asthma in Sudanese.},
year = {2023}
}
TY - JOUR T1 - Reactivity Skin-Prick Test with Common Allergens and Their Genetic Association with Polymorphisms in IL-9, IL-13, IL-4, IL-5 & IL-4Rα Genes among Asthmatics in Sudan AU - Amel Osman Gundi AU - Fatima Omer Hamed AU - Maha Hassan Agraa AU - Hiba Salaheldin Mohamed Y1 - 2023/06/05 PY - 2023 N1 - https://doi.org/10.11648/j.bmb.20230802.11 DO - 10.11648/j.bmb.20230802.11 T2 - Biochemistry and Molecular Biology JF - Biochemistry and Molecular Biology JO - Biochemistry and Molecular Biology SP - 21 EP - 28 PB - Science Publishing Group SN - 2575-5048 UR - https://doi.org/10.11648/j.bmb.20230802.11 AB - Atopy may be defined as the production of abnormal amounts of Immunoglobulin E (IgE) antibodies in response to aeroallergens, assessed by positive skin-prick test (SPT) responses. Allergic asthma is the result of a complex multifactorial interplay between genes and the environment. The interaction of genetic and environmental factors may increase the complexity associated with allergic asthma. We investigated the degree of association of skin reactivity and their association with seven polymorphisms in IL-4, IL-5, IL-13, and IL-9 genes on chromosome 5q31-q33 region, and three polymorphisms in the IL-4Rα gene on chromosome 16 p contributing to allergic asthma in a population-based sample of Sudanese families. 70 families, including (150 cases and 140 controls), were recruited. Asthmatic status was confirmed by pulmonary function tests (PFTs); a SPT was done using 14 aeroallergens. Genotyping for 10 single-nucleotide polymorphisms (SNPs); one SNP in the IL-4 gene; one SNP in the IL-5 gene; two SNPs in the IL-13 gene; three SNPs in the IL-9 gene, three SNPs in the IL-4Rα gene were obtained by using multiplex PCR Mass ARRAY matrix MALDI-TOF mass spectrometry;. For the SPT reactivity, out of the 14 allergens, seven showed a significant hypersensitivity in the majority of asthmatics, including; house dust mites (HDM), mixed moulds, D. Peterenyssinus (DP), mixed ragweed, grass pollens, and cat hair. The strongest sensitizer was HDM (P = 0.001). The analysis of SNPs for association with SPT reactivity revealed the following; the SNPs in IL-13 in addition, IL-4Rα showed evidence association with SPT reactivity symptoms, but SNPs in the IL-4 gene associated with marginally significant (P = 0.05). An interaction of (A−646G) allele of IL-13 with minor alleles of IL-5, IL-9, and IL-4Rα genes, showed a significant association with reactivity SPT. Asthmatics showed hypersensitivity to common aeroallergens, the more prevalent allergen was mixed moulds; the most significant one was HDM. More reactive alleles associated with skin reactivity were; (A-G) of the IL-13 gene and (G-A) of the IL-4R gene. An interaction of the IL-13 gene with IL-5, IL-9, and IL-4Rα genes, have a significant association with reactivity SPT. There is a correlation between the IL-13 gene, atopy, and asthma in Sudanese. VL - 8 IS - 2 ER -
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