Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins

Verônica Lisboa Santos; Juliana Bueno da Silva; Franciane Cristina de Figueiredo; Carlos Paulo Henrique Ronchi; Edenilse Gopinger

doi:doi:10.11648/j.avs.20221004.11

Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger

Published in Animal and Veterinary Sciences (Volume 10, Issue 4)

Received: 27 June 2022 Accepted: 14 July 2022 Published: 26 July 2022

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Abstract

Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.

Published in	Animal and Veterinary Sciences (Volume 10, Issue 4)
DOI	10.11648/j.avs.20221004.11
Page(s)	78-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Beta-Glucans, Immunity, Interleukin, Silymarin, Organic Selenium

References

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APA Style

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger. (2022). Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Animal and Veterinary Sciences, 10(4), 78-82. https://doi.org/10.11648/j.avs.20221004.11

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ACS Style

Verônica Lisboa Santos; Juliana Bueno da Silva; Franciane Cristina de Figueiredo; Carlos Paulo Henrique Ronchi; Edenilse Gopinger. Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Anim. Vet. Sci. 2022, 10(4), 78-82. doi: 10.11648/j.avs.20221004.11

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AMA Style

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger. Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Anim Vet Sci. 2022;10(4):78-82. doi: 10.11648/j.avs.20221004.11

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@article{10.11648/j.avs.20221004.11,
  author = {Verônica Lisboa Santos and Juliana Bueno da Silva and Franciane Cristina de Figueiredo and Carlos Paulo Henrique Ronchi and Edenilse Gopinger},
  title = {Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins},
  journal = {Animal and Veterinary Sciences},
  volume = {10},
  number = {4},
  pages = {78-82},
  doi = {10.11648/j.avs.20221004.11},
  url = {https://doi.org/10.11648/j.avs.20221004.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20221004.11},
  abstract = {Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.},
 year = {2022}
}

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TY  - JOUR
T1  - Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins
AU  - Verônica Lisboa Santos
AU  - Juliana Bueno da Silva
AU  - Franciane Cristina de Figueiredo
AU  - Carlos Paulo Henrique Ronchi
AU  - Edenilse Gopinger
Y1  - 2022/07/26
PY  - 2022
N1  - https://doi.org/10.11648/j.avs.20221004.11
DO  - 10.11648/j.avs.20221004.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 78
EP  - 82
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20221004.11
AB  - Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.
VL  - 10
IS  - 4
ER  -

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Author Information

Verônica Lisboa Santos
Juliana Bueno da Silva

Yessinergy do Brasil Agroindustrial Ltda, S?o Paulo, Brazil
Franciane Cristina de Figueiredo

Yessinergy do Brasil Agroindustrial Ltda, S?o Paulo, Brazil
Carlos Paulo Henrique Ronchi

Yessinergy do Brasil Agroindustrial Ltda, S?o Paulo, Brazil
Edenilse Gopinger

Zootechnics Department, Federal University of Pelotas, Pelotas, Brazil

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APA Style

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger. (2022). Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Animal and Veterinary Sciences, 10(4), 78-82. https://doi.org/10.11648/j.avs.20221004.11

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ACS Style

Verônica Lisboa Santos; Juliana Bueno da Silva; Franciane Cristina de Figueiredo; Carlos Paulo Henrique Ronchi; Edenilse Gopinger. Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Anim. Vet. Sci. 2022, 10(4), 78-82. doi: 10.11648/j.avs.20221004.11

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AMA Style

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger. Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Anim Vet Sci. 2022;10(4):78-82. doi: 10.11648/j.avs.20221004.11

Copy | Download

@article{10.11648/j.avs.20221004.11,
  author = {Verônica Lisboa Santos and Juliana Bueno da Silva and Franciane Cristina de Figueiredo and Carlos Paulo Henrique Ronchi and Edenilse Gopinger},
  title = {Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins},
  journal = {Animal and Veterinary Sciences},
  volume = {10},
  number = {4},
  pages = {78-82},
  doi = {10.11648/j.avs.20221004.11},
  url = {https://doi.org/10.11648/j.avs.20221004.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20221004.11},
  abstract = {Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins
AU  - Verônica Lisboa Santos
AU  - Juliana Bueno da Silva
AU  - Franciane Cristina de Figueiredo
AU  - Carlos Paulo Henrique Ronchi
AU  - Edenilse Gopinger
Y1  - 2022/07/26
PY  - 2022
N1  - https://doi.org/10.11648/j.avs.20221004.11
DO  - 10.11648/j.avs.20221004.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 78
EP  - 82
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20221004.11
AB  - Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.
VL  - 10
IS  - 4
ER  -

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