Foot-and-mouth disease is an endemic and economically important, contagious viral infection of cloven-hoofed animals that poses a huge economic impact. This study was an outbreak investigation study to detect and isolate the circulating foot-and-mouth disease serotype in central Ethiopia between December 2020 and March 2021. For this 35-sample outbreak (26 epithelium and 9 swabs), samples were purposively collected. Virus isolation was performed on baby hamster kidney cells line (BHK-21), followed by nucleic acid detection of the agent in the samples using real-time and conventional polymerase chain reactions. Serotyping was done by antigen detection sandwich enzyme-linked immunosorbent assay (ELISA). In the present study, out of 35 samples, only 24 (68.75%) showed a cytopathic effect on a monolayer of the BHK-21 cell line. The nucleic acid of the virus was detected in 24 samples targeting a specific gene called 3D Pol. With regard to the serotype distribution, four serotypes were identified (O, A, SAT2, and SAT1). The dominant serotype circulating in the study area was serotype O (87.5%), followed by serotype A (25%), SAT2 (12.5%), and SAT1 (12.5%). Based on this finding, we recommend that regular outbreak investigation encompassing all the regions and districts be done in order to identify the circulating serotype and that further sequence based analysis of the topotypes be done. This will help with the prevention and control of the diseases through vaccination.
| Published in | American Journal of Life Sciences (Volume 11, Issue 3) |
| DOI | 10.11648/j.ajls.20231103.12 |
| Page(s) | 36-43 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Foot-and-Mouth Disease, Virus, Ethiopia
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APA Style
Demessa Negessu, Ibsa Ebisa, Abde Mohamad, Tsion Bilata, Ayelech Muluneh, et al. (2023). Isolation, Molecular Detection and Genotyping of Foot-and-Mouth Disease Virus from Naturally Infected Cattle in Central Ethiopia. American Journal of Life Sciences, 11(3), 36-43. https://doi.org/10.11648/j.ajls.20231103.12
ACS Style
Demessa Negessu; Ibsa Ebisa; Abde Mohamad; Tsion Bilata; Ayelech Muluneh, et al. Isolation, Molecular Detection and Genotyping of Foot-and-Mouth Disease Virus from Naturally Infected Cattle in Central Ethiopia. Am. J. Life Sci. 2023, 11(3), 36-43. doi: 10.11648/j.ajls.20231103.12
AMA Style
Demessa Negessu, Ibsa Ebisa, Abde Mohamad, Tsion Bilata, Ayelech Muluneh, et al. Isolation, Molecular Detection and Genotyping of Foot-and-Mouth Disease Virus from Naturally Infected Cattle in Central Ethiopia. Am J Life Sci. 2023;11(3):36-43. doi: 10.11648/j.ajls.20231103.12
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Download@article{10.11648/j.ajls.20231103.12,
author = {Demessa Negessu and Ibsa Ebisa and Abde Mohamad and Tsion Bilata and Ayelech Muluneh and Dereje Shagu and Bekele Yalew and Fanos Tadesse Woldemariyam},
title = {Isolation, Molecular Detection and Genotyping of Foot-and-Mouth Disease Virus from Naturally Infected Cattle in Central Ethiopia},
journal = {American Journal of Life Sciences},
volume = {11},
number = {3},
pages = {36-43},
doi = {10.11648/j.ajls.20231103.12},
url = {https://doi.org/10.11648/j.ajls.20231103.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20231103.12},
abstract = {Foot-and-mouth disease is an endemic and economically important, contagious viral infection of cloven-hoofed animals that poses a huge economic impact. This study was an outbreak investigation study to detect and isolate the circulating foot-and-mouth disease serotype in central Ethiopia between December 2020 and March 2021. For this 35-sample outbreak (26 epithelium and 9 swabs), samples were purposively collected. Virus isolation was performed on baby hamster kidney cells line (BHK-21), followed by nucleic acid detection of the agent in the samples using real-time and conventional polymerase chain reactions. Serotyping was done by antigen detection sandwich enzyme-linked immunosorbent assay (ELISA). In the present study, out of 35 samples, only 24 (68.75%) showed a cytopathic effect on a monolayer of the BHK-21 cell line. The nucleic acid of the virus was detected in 24 samples targeting a specific gene called 3D Pol. With regard to the serotype distribution, four serotypes were identified (O, A, SAT2, and SAT1). The dominant serotype circulating in the study area was serotype O (87.5%), followed by serotype A (25%), SAT2 (12.5%), and SAT1 (12.5%). Based on this finding, we recommend that regular outbreak investigation encompassing all the regions and districts be done in order to identify the circulating serotype and that further sequence based analysis of the topotypes be done. This will help with the prevention and control of the diseases through vaccination.},
year = {2023}
}
TY - JOUR T1 - Isolation, Molecular Detection and Genotyping of Foot-and-Mouth Disease Virus from Naturally Infected Cattle in Central Ethiopia AU - Demessa Negessu AU - Ibsa Ebisa AU - Abde Mohamad AU - Tsion Bilata AU - Ayelech Muluneh AU - Dereje Shagu AU - Bekele Yalew AU - Fanos Tadesse Woldemariyam Y1 - 2023/06/27 PY - 2023 N1 - https://doi.org/10.11648/j.ajls.20231103.12 DO - 10.11648/j.ajls.20231103.12 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 36 EP - 43 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20231103.12 AB - Foot-and-mouth disease is an endemic and economically important, contagious viral infection of cloven-hoofed animals that poses a huge economic impact. This study was an outbreak investigation study to detect and isolate the circulating foot-and-mouth disease serotype in central Ethiopia between December 2020 and March 2021. For this 35-sample outbreak (26 epithelium and 9 swabs), samples were purposively collected. Virus isolation was performed on baby hamster kidney cells line (BHK-21), followed by nucleic acid detection of the agent in the samples using real-time and conventional polymerase chain reactions. Serotyping was done by antigen detection sandwich enzyme-linked immunosorbent assay (ELISA). In the present study, out of 35 samples, only 24 (68.75%) showed a cytopathic effect on a monolayer of the BHK-21 cell line. The nucleic acid of the virus was detected in 24 samples targeting a specific gene called 3D Pol. With regard to the serotype distribution, four serotypes were identified (O, A, SAT2, and SAT1). The dominant serotype circulating in the study area was serotype O (87.5%), followed by serotype A (25%), SAT2 (12.5%), and SAT1 (12.5%). Based on this finding, we recommend that regular outbreak investigation encompassing all the regions and districts be done in order to identify the circulating serotype and that further sequence based analysis of the topotypes be done. This will help with the prevention and control of the diseases through vaccination. VL - 11 IS - 3 ER -
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