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Retention of Seed Storage Potential Using Ascorbic Acid

Received: 12 March 2022    Accepted: 30 March 2022    Published: 12 May 2022
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Abstract

An investigation was carried out on prolongation of seed vigour of a black gram species by using a selected chemical. Black gram seeds lost viability at a rapid pace under accelerated ageing condition. Pretreatment of black gram (Vigna mungo L.) seeds with ascorbic acid for 6 hours (3+3) before accelerated ageing treatment (100% RH and 32±2°C) for different durations (0 to 30 days) slowed down the ageing-induced rapid loss of germination. The chemical also significantly arrested the reduction of protein, insoluble carbohydrate levels as well as activity of catalase enzyme of seed kernels during forced ageing period was ameliorated to a significant extent in the chemical-pretreated seed. Conversely, ageing-induced stimulation of the activity of amylase enzyme was alleviated by the seed pretreating agent. Seed potential was found to be much better in the pretreatments as evidenced from the treatment-induced higher protein and activity of catalase enzyme in spite of adverse storage situation. Results, therefore, pointed out that the ascorbic acid pretreated seeds retained higher seed vigour of black gram species. The promising effects of the experimental chemical on storage potentiation of the seed is apparent in this investigation.

Published in American Journal of Life Sciences (Volume 10, Issue 2)
DOI 10.11648/j.ajls.20221002.12
Page(s) 28-30
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Black Gram, Ascorbic Acid, Catalase, Seed Potential, Accelerated Ageing

References
[1] Pati CK. & Bhattacharjee A. (2012). Sunflower seed invigoration by chemical manipulation, Agricultural Journal, 7 (1): 26-31.
[2] Christensen CM. & Kaufmann HH. (1965). Deterioration of stored grain by fungi, Annal Review of Phytopathology, 3: 69-84.
[3] Aziz NH. & Shair AAM. (1997). Influence of other fungi on aflatoxin production by Aspergillus flavus in maize kernels, Journal of Food Safety, 17 (2): 113-123.
[4] Copelan LO &, M. B. McDonald MB. (1995). Principles of Seed Science and Technology, (3rd ed.), Chapman and Hall, New York.
[5] Ojha S., Pati CK. & A. Bhattacharjee A. (2012). Seed invigouration and plant potentiation of two pulse crop cultivars under stressful storage condition, Journal of Botanical Society of Bengal, 66 (1): 63-67.
[6] Chhetri DR., Rai AS &, A. Bhattacharjee A. (1993). Chemical manipulation of seed longevity of four crop species in an unfavourable storage environment, Seed Science and Technology, 21: 31-44.
[7] Basu RN. (1994). An appraisal of research on wet and dry physiological seed treatments and their applicability with special reference to tropical and sub-tropical countries, Seed Science and Technology, 22: 107-126.
[8] Pati CK. & Bhattacharjee A. (2013). Chemical Manipulation for Storage Potentiation of Crop Seeds, LAP LAMBERT Academic Publishing, Germany.
[9] Heydecker W. (1972. Vigour in Viability of Seeds, (ed. E. H. Roberts), pp. 209-252. Chapmann and Hall Ltd., London.
[10] Pati CK., Mishra VK. & Bhattacharjee A. (2004). Problem of seed storage of grass pea and black gram cultivars under stressful storage environment, Journal of Science and Technology, XVI (A): 11-17.
[11] Pati CK. (2007). Seed invigouration, plant potentiation and yield augmentation of two promising pulse crops (Lathurus sativus L. and Vinna mungo (L.) Hepper) by chemical manipulation, Ph.D. thesis, Vidyasagar University, West Bengal, India.
[12] International Seed Testing Association. (1976). International Rules for Seed Testing, Seed Science and Technology, 4: 51-177.
[13] Coolbear P., Francis A. & Grierson D. (1984). The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds, Journal of Experimental Botany, 35: 1609-1617.
[14] McCready, RM, Guggloz. J, Silviera, V and Owens, HS. (1950). Determination of starch and amylase in vegetables. Analyt. Chem. 22: 1156-1158.
[15] Lowry OH., Rosebrough NJ., Farr, AL. & Randall RJ. (1951). Protein measurement with the Folin-phenol reagent, Journal of Biological Chemistry, 193: 265-275.
[16] Snell FD. & Snell CT. (1971). Colorimetric methods of analysis, 4AAA: 7-145. Van Nostrand Reinhold Co., New York.
[17] Khan AA. & Faust MA. (1967). Effect of growth retardants on a-amylase production in germinating barley seeds, Physiologia Plantarum, 20: 673-681.
[18] Fick NG. & Qualset CO. (1975). Genetic control of endosperm amylase activity and gibberellin responses in standard height and short statured wheat, Proceedings of National Academy of Science, USA, 72: 892-895.
[19] Panse VG. & Sukhatme PT. (1967). Statistical methods for agricultural workers, 2ed., pp. 150-157, Indian Council of Agricultural Research, New Delhi.
[20] Powell AA. & Matthews S. (1977). Deteriorative changes in pea seeds stored in humid or dry conditions, Journal of Experimental Botany, 28: 225-234.
[21] Pati CK. (2020). Enhancement of Plant Potential using IAA, International Research Journal of Biological Sciences, 9 (1): 25-26.
[22] Pati CK. & A. Bhattacharjee A. (2014). Prolongation of seed vigour of maize species under stressful storage environment using selected chemicals, Indian Journal of Research in Multidisciplinary Studies, 1 (1): 16-22.
[23] Simon EW. (1974). Phospholipids and plant membrane permeability, New Phytologist, 73: 377-420.
[24] Abdul-Baki AA. & Anderson JD. (1972). Physiological and biochemical deterioration of seeds, In Seed Biology (ed. T. T. Kozlowski), 2: 283-315, Academic Press, New York.
[25] Kole S. & Gupta K. (1982). Biochemical changes in safflower (Carthamus tinctorius) seeds under accelerated ageing, Seed Science and Technology, 10: 47-54.
[26] Fridovich I. (1976). Oxygen radicals, hydrogen peroxide, and oxygen toxicity. In Free Radicals in Biology, (ed. W.A. Prior), Vol. 1 pp. 239-277, Academic Press, New York.
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    Chandan Kumar Pati. (2022). Retention of Seed Storage Potential Using Ascorbic Acid. American Journal of Life Sciences, 10(2), 28-30. https://doi.org/10.11648/j.ajls.20221002.12

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    Chandan Kumar Pati. Retention of Seed Storage Potential Using Ascorbic Acid. Am. J. Life Sci. 2022, 10(2), 28-30. doi: 10.11648/j.ajls.20221002.12

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    AMA Style

    Chandan Kumar Pati. Retention of Seed Storage Potential Using Ascorbic Acid. Am J Life Sci. 2022;10(2):28-30. doi: 10.11648/j.ajls.20221002.12

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  • @article{10.11648/j.ajls.20221002.12,
      author = {Chandan Kumar Pati},
      title = {Retention of Seed Storage Potential Using Ascorbic Acid},
      journal = {American Journal of Life Sciences},
      volume = {10},
      number = {2},
      pages = {28-30},
      doi = {10.11648/j.ajls.20221002.12},
      url = {https://doi.org/10.11648/j.ajls.20221002.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20221002.12},
      abstract = {An investigation was carried out on prolongation of seed vigour of a black gram species by using a selected chemical. Black gram seeds lost viability at a rapid pace under accelerated ageing condition. Pretreatment of black gram (Vigna mungo L.) seeds with ascorbic acid for 6 hours (3+3) before accelerated ageing treatment (100% RH and 32±2°C) for different durations (0 to 30 days) slowed down the ageing-induced rapid loss of germination. The chemical also significantly arrested the reduction of protein, insoluble carbohydrate levels as well as activity of catalase enzyme of seed kernels during forced ageing period was ameliorated to a significant extent in the chemical-pretreated seed. Conversely, ageing-induced stimulation of the activity of amylase enzyme was alleviated by the seed pretreating agent. Seed potential was found to be much better in the pretreatments as evidenced from the treatment-induced higher protein and activity of catalase enzyme in spite of adverse storage situation. Results, therefore, pointed out that the ascorbic acid pretreated seeds retained higher seed vigour of black gram species. The promising effects of the experimental chemical on storage potentiation of the seed is apparent in this investigation.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Retention of Seed Storage Potential Using Ascorbic Acid
    AU  - Chandan Kumar Pati
    Y1  - 2022/05/12
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ajls.20221002.12
    DO  - 10.11648/j.ajls.20221002.12
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 28
    EP  - 30
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20221002.12
    AB  - An investigation was carried out on prolongation of seed vigour of a black gram species by using a selected chemical. Black gram seeds lost viability at a rapid pace under accelerated ageing condition. Pretreatment of black gram (Vigna mungo L.) seeds with ascorbic acid for 6 hours (3+3) before accelerated ageing treatment (100% RH and 32±2°C) for different durations (0 to 30 days) slowed down the ageing-induced rapid loss of germination. The chemical also significantly arrested the reduction of protein, insoluble carbohydrate levels as well as activity of catalase enzyme of seed kernels during forced ageing period was ameliorated to a significant extent in the chemical-pretreated seed. Conversely, ageing-induced stimulation of the activity of amylase enzyme was alleviated by the seed pretreating agent. Seed potential was found to be much better in the pretreatments as evidenced from the treatment-induced higher protein and activity of catalase enzyme in spite of adverse storage situation. Results, therefore, pointed out that the ascorbic acid pretreated seeds retained higher seed vigour of black gram species. The promising effects of the experimental chemical on storage potentiation of the seed is apparent in this investigation.
    VL  - 10
    IS  - 2
    ER  - 

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Author Information
  • Department of Botany, Saldiha College (Affiliated to Bankura University), Bankura, India

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