<i>Khaya grandifololia</i> Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats

Fils Armand Ella; Simon Ngamli Fewou; Mario Robillard Didier Koloko; Pascal Owona; Frederic Nico Njayou; Paul Fewou Moundipa

doi:doi:10.11648/j.ajbls.20221003.15

Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats

Fils Armand Ella, Simon Ngamli Fewou, Mario Robillard Didier Koloko, Pascal Owona, Frederic Nico Njayou, Paul Fewou Moundipa

Published in American Journal of Biomedical and Life Sciences (Volume 10, Issue 3)

Received: 23 May 2022 Accepted: 14 June 2022 Published: 27 June 2022

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Abstract

Alzheimer’s disease (AD) is a multifactorial, progressive neurodegenerative disorder with dementia and persistent impairment of cognitive functions as the main clinical characteristics. Although signs of progress are being made in developing AD therapy, there is no effective drug capable of stopping and/or slowing down the progression of the disease. We have previously indicated in an in vitro setting of AD that Khaya grandifololia (KG) crude extract possesses antioxidant, anti-inflammatory, and neuroprotective activities. In the current work, we have evaluated the activity of KG hydroethanolic (KG-HE) extract in preventing cognitive impairment and promoting memory improvement in vivo. Results from behavioral tests indicated a significant improvement in memory performance and a delay in depression-like behavior upon treatment of rats with KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) as standard. Because scopolamine (1 mg/kg) impaired cognitive performance in the tail suspension test, Morris Water Maze test, and Novelty Suppressed Feeding Test, KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) treatment prevented scopolamine-induced performance impairment. Moreover, both KG-HE extract (5, 25, and 50 mg/kg) and donepezil (1 mg/kg) prevented the scopolamine-induced cognitive impairment by inhibiting the acetylcholinesterase activity. In addition, the brain parameters of stress oxidation (SOD, CAT, and GSH) reduced by scopolamine treatment were regulated by the administration of KG-HE extract or the standard drug donepezil. An increase in the MDA level and the phosphatase activity both in the serum and brain due to scopolamine treatment was restored by the administration of KG-HE extract or donepezil. Taken together, these results suggest that KG-HE extract improves cognition and relieves the scopolamine-induced cognitive impairment via activation of the cholinergic and anti-oxidation systems in rats.

Published in	American Journal of Biomedical and Life Sciences (Volume 10, Issue 3)
DOI	10.11648/j.ajbls.20221003.15
Page(s)	84-96
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Khaya grandifololia Hydroethanolic Extract, Alzheimer Disease, Cognition, Neuroprotection, Anxiety, Depression, Cholinergic System, Stress Oxidation

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Fils Armand Ella, Simon Ngamli Fewou, Mario Robillard Didier Koloko, Pascal Owona, Frederic Nico Njayou, et al. (2022). Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats. American Journal of Biomedical and Life Sciences, 10(3), 84-96. https://doi.org/10.11648/j.ajbls.20221003.15

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Fils Armand Ella; Simon Ngamli Fewou; Mario Robillard Didier Koloko; Pascal Owona; Frederic Nico Njayou, et al. Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats. Am. J. Biomed. Life Sci. 2022, 10(3), 84-96. doi: 10.11648/j.ajbls.20221003.15

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Fils Armand Ella, Simon Ngamli Fewou, Mario Robillard Didier Koloko, Pascal Owona, Frederic Nico Njayou, et al. Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats. Am J Biomed Life Sci. 2022;10(3):84-96. doi: 10.11648/j.ajbls.20221003.15

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@article{10.11648/j.ajbls.20221003.15,
  author = {Fils Armand Ella and Simon Ngamli Fewou and Mario Robillard Didier Koloko and Pascal Owona and Frederic Nico Njayou and Paul Fewou Moundipa},
  title = {Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {10},
  number = {3},
  pages = {84-96},
  doi = {10.11648/j.ajbls.20221003.15},
  url = {https://doi.org/10.11648/j.ajbls.20221003.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221003.15},
  abstract = {Alzheimer’s disease (AD) is a multifactorial, progressive neurodegenerative disorder with dementia and persistent impairment of cognitive functions as the main clinical characteristics. Although signs of progress are being made in developing AD therapy, there is no effective drug capable of stopping and/or slowing down the progression of the disease. We have previously indicated in an in vitro setting of AD that Khaya grandifololia (KG) crude extract possesses antioxidant, anti-inflammatory, and neuroprotective activities. In the current work, we have evaluated the activity of KG hydroethanolic (KG-HE) extract in preventing cognitive impairment and promoting memory improvement in vivo. Results from behavioral tests indicated a significant improvement in memory performance and a delay in depression-like behavior upon treatment of rats with KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) as standard. Because scopolamine (1 mg/kg) impaired cognitive performance in the tail suspension test, Morris Water Maze test, and Novelty Suppressed Feeding Test, KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) treatment prevented scopolamine-induced performance impairment. Moreover, both KG-HE extract (5, 25, and 50 mg/kg) and donepezil (1 mg/kg) prevented the scopolamine-induced cognitive impairment by inhibiting the acetylcholinesterase activity. In addition, the brain parameters of stress oxidation (SOD, CAT, and GSH) reduced by scopolamine treatment were regulated by the administration of KG-HE extract or the standard drug donepezil. An increase in the MDA level and the phosphatase activity both in the serum and brain due to scopolamine treatment was restored by the administration of KG-HE extract or donepezil. Taken together, these results suggest that KG-HE extract improves cognition and relieves the scopolamine-induced cognitive impairment via activation of the cholinergic and anti-oxidation systems in rats.},
 year = {2022}
}

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TY  - JOUR
T1  - Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats
AU  - Fils Armand Ella
AU  - Simon Ngamli Fewou
AU  - Mario Robillard Didier Koloko
AU  - Pascal Owona
AU  - Frederic Nico Njayou
AU  - Paul Fewou Moundipa
Y1  - 2022/06/27
PY  - 2022
N1  - https://doi.org/10.11648/j.ajbls.20221003.15
DO  - 10.11648/j.ajbls.20221003.15
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 84
EP  - 96
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20221003.15
AB  - Alzheimer’s disease (AD) is a multifactorial, progressive neurodegenerative disorder with dementia and persistent impairment of cognitive functions as the main clinical characteristics. Although signs of progress are being made in developing AD therapy, there is no effective drug capable of stopping and/or slowing down the progression of the disease. We have previously indicated in an in vitro setting of AD that Khaya grandifololia (KG) crude extract possesses antioxidant, anti-inflammatory, and neuroprotective activities. In the current work, we have evaluated the activity of KG hydroethanolic (KG-HE) extract in preventing cognitive impairment and promoting memory improvement in vivo. Results from behavioral tests indicated a significant improvement in memory performance and a delay in depression-like behavior upon treatment of rats with KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) as standard. Because scopolamine (1 mg/kg) impaired cognitive performance in the tail suspension test, Morris Water Maze test, and Novelty Suppressed Feeding Test, KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) treatment prevented scopolamine-induced performance impairment. Moreover, both KG-HE extract (5, 25, and 50 mg/kg) and donepezil (1 mg/kg) prevented the scopolamine-induced cognitive impairment by inhibiting the acetylcholinesterase activity. In addition, the brain parameters of stress oxidation (SOD, CAT, and GSH) reduced by scopolamine treatment were regulated by the administration of KG-HE extract or the standard drug donepezil. An increase in the MDA level and the phosphatase activity both in the serum and brain due to scopolamine treatment was restored by the administration of KG-HE extract or donepezil. Taken together, these results suggest that KG-HE extract improves cognition and relieves the scopolamine-induced cognitive impairment via activation of the cholinergic and anti-oxidation systems in rats.
VL  - 10
IS  - 3
ER  -

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Fils Armand Ella

Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
Simon Ngamli Fewou

Faculty of Health Science, Université des Montagnes, Bangangté, Cameroon
Mario Robillard Didier Koloko

Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
Pascal Owona

Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
Frederic Nico Njayou

Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
Paul Fewou Moundipa

Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon

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APA Style

Fils Armand Ella, Simon Ngamli Fewou, Mario Robillard Didier Koloko, Pascal Owona, Frederic Nico Njayou, et al. (2022). Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats. American Journal of Biomedical and Life Sciences, 10(3), 84-96. https://doi.org/10.11648/j.ajbls.20221003.15

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ACS Style

Fils Armand Ella; Simon Ngamli Fewou; Mario Robillard Didier Koloko; Pascal Owona; Frederic Nico Njayou, et al. Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats. Am. J. Biomed. Life Sci. 2022, 10(3), 84-96. doi: 10.11648/j.ajbls.20221003.15

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AMA Style

Fils Armand Ella, Simon Ngamli Fewou, Mario Robillard Didier Koloko, Pascal Owona, Frederic Nico Njayou, et al. Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats. Am J Biomed Life Sci. 2022;10(3):84-96. doi: 10.11648/j.ajbls.20221003.15

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@article{10.11648/j.ajbls.20221003.15,
  author = {Fils Armand Ella and Simon Ngamli Fewou and Mario Robillard Didier Koloko and Pascal Owona and Frederic Nico Njayou and Paul Fewou Moundipa},
  title = {Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {10},
  number = {3},
  pages = {84-96},
  doi = {10.11648/j.ajbls.20221003.15},
  url = {https://doi.org/10.11648/j.ajbls.20221003.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221003.15},
  abstract = {Alzheimer’s disease (AD) is a multifactorial, progressive neurodegenerative disorder with dementia and persistent impairment of cognitive functions as the main clinical characteristics. Although signs of progress are being made in developing AD therapy, there is no effective drug capable of stopping and/or slowing down the progression of the disease. We have previously indicated in an in vitro setting of AD that Khaya grandifololia (KG) crude extract possesses antioxidant, anti-inflammatory, and neuroprotective activities. In the current work, we have evaluated the activity of KG hydroethanolic (KG-HE) extract in preventing cognitive impairment and promoting memory improvement in vivo. Results from behavioral tests indicated a significant improvement in memory performance and a delay in depression-like behavior upon treatment of rats with KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) as standard. Because scopolamine (1 mg/kg) impaired cognitive performance in the tail suspension test, Morris Water Maze test, and Novelty Suppressed Feeding Test, KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) treatment prevented scopolamine-induced performance impairment. Moreover, both KG-HE extract (5, 25, and 50 mg/kg) and donepezil (1 mg/kg) prevented the scopolamine-induced cognitive impairment by inhibiting the acetylcholinesterase activity. In addition, the brain parameters of stress oxidation (SOD, CAT, and GSH) reduced by scopolamine treatment were regulated by the administration of KG-HE extract or the standard drug donepezil. An increase in the MDA level and the phosphatase activity both in the serum and brain due to scopolamine treatment was restored by the administration of KG-HE extract or donepezil. Taken together, these results suggest that KG-HE extract improves cognition and relieves the scopolamine-induced cognitive impairment via activation of the cholinergic and anti-oxidation systems in rats.},
 year = {2022}
}

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TY  - JOUR
T1  - Khaya grandifololia Improves Cognition and Prevents Scopolamine-Induced Impairment of Brain Functions by Activating the Cholinergic and Antioxidant Systems in Rats
AU  - Fils Armand Ella
AU  - Simon Ngamli Fewou
AU  - Mario Robillard Didier Koloko
AU  - Pascal Owona
AU  - Frederic Nico Njayou
AU  - Paul Fewou Moundipa
Y1  - 2022/06/27
PY  - 2022
N1  - https://doi.org/10.11648/j.ajbls.20221003.15
DO  - 10.11648/j.ajbls.20221003.15
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 84
EP  - 96
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20221003.15
AB  - Alzheimer’s disease (AD) is a multifactorial, progressive neurodegenerative disorder with dementia and persistent impairment of cognitive functions as the main clinical characteristics. Although signs of progress are being made in developing AD therapy, there is no effective drug capable of stopping and/or slowing down the progression of the disease. We have previously indicated in an in vitro setting of AD that Khaya grandifololia (KG) crude extract possesses antioxidant, anti-inflammatory, and neuroprotective activities. In the current work, we have evaluated the activity of KG hydroethanolic (KG-HE) extract in preventing cognitive impairment and promoting memory improvement in vivo. Results from behavioral tests indicated a significant improvement in memory performance and a delay in depression-like behavior upon treatment of rats with KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) as standard. Because scopolamine (1 mg/kg) impaired cognitive performance in the tail suspension test, Morris Water Maze test, and Novelty Suppressed Feeding Test, KG-HE extract (5, 25, and 50 mg/kg) or donepezil (1 mg/kg) treatment prevented scopolamine-induced performance impairment. Moreover, both KG-HE extract (5, 25, and 50 mg/kg) and donepezil (1 mg/kg) prevented the scopolamine-induced cognitive impairment by inhibiting the acetylcholinesterase activity. In addition, the brain parameters of stress oxidation (SOD, CAT, and GSH) reduced by scopolamine treatment were regulated by the administration of KG-HE extract or the standard drug donepezil. An increase in the MDA level and the phosphatase activity both in the serum and brain due to scopolamine treatment was restored by the administration of KG-HE extract or donepezil. Taken together, these results suggest that KG-HE extract improves cognition and relieves the scopolamine-induced cognitive impairment via activation of the cholinergic and anti-oxidation systems in rats.
VL  - 10
IS  - 3
ER  -

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