In this study, in vitro and in vivo experiments, it was studied soforaflavonozide (SFL) isolated from Alhagi canescens (Regel) B. Keller & Shap (Fabaceae (Leguminosae)), and narcissine isolated from Crocus sativus L. belonging to Iridaceae family. It was studied the effect of narcissine isolated from the plant on ATP-dependent potassium channel (mitoKATP-channel) activity in rat cardiac mitochondria. Animals of experimental group were divided into 4 groups: I control group (healthy), II experimental group (ischemia model), III experiment group (ischemia + narcissin), IV experimental group (ischemia + SFL). In rats with ischemia a 0.1 ml 0.1% solution of 100 mg/kg adrenaline was administered subcutaneously and peritoneally for 3 days in relative to body weight. The rats that underwent the ischemia model were given oral administration of 10 mg/kg of narcissine flavonoid to group III and 10 mg/kg of SFL flavonoid to group IV orally for 7 days. After that, in the experimental animals carried out electrocardiogram. Mitochondria from rat heart tissue were isolated by differential centrifugation. Cardiac mitoKATP-channel activity in the presence of ATP in an incubation medium was studied at concentrations of 10–50 μM of SFL and narcissine. Concentrations of 50 μM of SFL and narcissus and 30 μM of diazoxide were also found to have an activating effect on the mitochondrial channel of the heart. In the adrenaline-induced ischemia model, it was found that narcissine and SFL flavonoids restored the mitochondrial conduction permeability of the rat heart.
| Published in | American Journal of Biomedical and Life Sciences (Volume 10, Issue 2) |
| DOI | 10.11648/j.ajbls.20221002.17 |
| Page(s) | 45-48 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Heart, Mitochondria, MitoKATP-channel, Adrenaline-induced Ischemia, Soforaflavonozide, Narcissine
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APA Style
Mirzaolimov Elmurod Ismoilovich, Pozilov Mamurjon Komiljonovich, Abduqaxarov Abdugaffor Soyibjon O’g’li, Tursunov Davronbek Rahmonjon O’g’li, Afzalova Sayyora Abdulahadovna, et al. (2022). Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model. American Journal of Biomedical and Life Sciences, 10(2), 45-48. https://doi.org/10.11648/j.ajbls.20221002.17
ACS Style
Mirzaolimov Elmurod Ismoilovich; Pozilov Mamurjon Komiljonovich; Abduqaxarov Abdugaffor Soyibjon O’g’li; Tursunov Davronbek Rahmonjon O’g’li; Afzalova Sayyora Abdulahadovna, et al. Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model. Am. J. Biomed. Life Sci. 2022, 10(2), 45-48. doi: 10.11648/j.ajbls.20221002.17
AMA Style
Mirzaolimov Elmurod Ismoilovich, Pozilov Mamurjon Komiljonovich, Abduqaxarov Abdugaffor Soyibjon O’g’li, Tursunov Davronbek Rahmonjon O’g’li, Afzalova Sayyora Abdulahadovna, et al. Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model. Am J Biomed Life Sci. 2022;10(2):45-48. doi: 10.11648/j.ajbls.20221002.17
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Download@article{10.11648/j.ajbls.20221002.17,
author = {Mirzaolimov Elmurod Ismoilovich and Pozilov Mamurjon Komiljonovich and Abduqaxarov Abdugaffor Soyibjon O’g’li and Tursunov Davronbek Rahmonjon O’g’li and Afzalova Sayyora Abdulahadovna and Nishanbaev Sabir Zaripbaevich},
title = {Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model},
journal = {American Journal of Biomedical and Life Sciences},
volume = {10},
number = {2},
pages = {45-48},
doi = {10.11648/j.ajbls.20221002.17},
url = {https://doi.org/10.11648/j.ajbls.20221002.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221002.17},
abstract = {In this study, in vitro and in vivo experiments, it was studied soforaflavonozide (SFL) isolated from Alhagi canescens (Regel) B. Keller & Shap (Fabaceae (Leguminosae)), and narcissine isolated from Crocus sativus L. belonging to Iridaceae family. It was studied the effect of narcissine isolated from the plant on ATP-dependent potassium channel (mitoKATP-channel) activity in rat cardiac mitochondria. Animals of experimental group were divided into 4 groups: I control group (healthy), II experimental group (ischemia model), III experiment group (ischemia + narcissin), IV experimental group (ischemia + SFL). In rats with ischemia a 0.1 ml 0.1% solution of 100 mg/kg adrenaline was administered subcutaneously and peritoneally for 3 days in relative to body weight. The rats that underwent the ischemia model were given oral administration of 10 mg/kg of narcissine flavonoid to group III and 10 mg/kg of SFL flavonoid to group IV orally for 7 days. After that, in the experimental animals carried out electrocardiogram. Mitochondria from rat heart tissue were isolated by differential centrifugation. Cardiac mitoKATP-channel activity in the presence of ATP in an incubation medium was studied at concentrations of 10–50 μM of SFL and narcissine. Concentrations of 50 μM of SFL and narcissus and 30 μM of diazoxide were also found to have an activating effect on the mitochondrial channel of the heart. In the adrenaline-induced ischemia model, it was found that narcissine and SFL flavonoids restored the mitochondrial conduction permeability of the rat heart.},
year = {2022}
}
TY - JOUR T1 - Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model AU - Mirzaolimov Elmurod Ismoilovich AU - Pozilov Mamurjon Komiljonovich AU - Abduqaxarov Abdugaffor Soyibjon O’g’li AU - Tursunov Davronbek Rahmonjon O’g’li AU - Afzalova Sayyora Abdulahadovna AU - Nishanbaev Sabir Zaripbaevich Y1 - 2022/04/22 PY - 2022 N1 - https://doi.org/10.11648/j.ajbls.20221002.17 DO - 10.11648/j.ajbls.20221002.17 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 45 EP - 48 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20221002.17 AB - In this study, in vitro and in vivo experiments, it was studied soforaflavonozide (SFL) isolated from Alhagi canescens (Regel) B. Keller & Shap (Fabaceae (Leguminosae)), and narcissine isolated from Crocus sativus L. belonging to Iridaceae family. It was studied the effect of narcissine isolated from the plant on ATP-dependent potassium channel (mitoKATP-channel) activity in rat cardiac mitochondria. Animals of experimental group were divided into 4 groups: I control group (healthy), II experimental group (ischemia model), III experiment group (ischemia + narcissin), IV experimental group (ischemia + SFL). In rats with ischemia a 0.1 ml 0.1% solution of 100 mg/kg adrenaline was administered subcutaneously and peritoneally for 3 days in relative to body weight. The rats that underwent the ischemia model were given oral administration of 10 mg/kg of narcissine flavonoid to group III and 10 mg/kg of SFL flavonoid to group IV orally for 7 days. After that, in the experimental animals carried out electrocardiogram. Mitochondria from rat heart tissue were isolated by differential centrifugation. Cardiac mitoKATP-channel activity in the presence of ATP in an incubation medium was studied at concentrations of 10–50 μM of SFL and narcissine. Concentrations of 50 μM of SFL and narcissus and 30 μM of diazoxide were also found to have an activating effect on the mitochondrial channel of the heart. In the adrenaline-induced ischemia model, it was found that narcissine and SFL flavonoids restored the mitochondrial conduction permeability of the rat heart. VL - 10 IS - 2 ER -
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