Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria

Mbah-Omeje Kelechi; Ugwu Celestina Chibuzor

doi:doi:10.11648/j.ajbls.20221002.14

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Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria

Mbah-Omeje Kelechi, Ugwu Celestina Chibuzor

Published in American Journal of Biomedical and Life Sciences (Volume 10, Issue 2)

Received: 23 February 2022 Accepted: 15 March 2022 Published: 23 March 2022

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Abstract

The prevalence rates of Mycoplasma hominis and Ureaplasma Spp and the evaluation of edible herbs against genital mycoplasmas were determined among university students in Enugu state, Nigeria. Specimens from 2400 subjects comprising of 1200 male and female subjects were tested for Mycoplasma hominis and Ureaplasma Spp. Cultures were done on Mycoplasma agar, A7agar and urea-arginine LYO2 broth. Antimicrobial susceptibility pattern was determined by Mycoplasma IST2 kit. Preliminary qualitative phytochemical analysis was done using standard methods to reveal the presence of basic phytochemicals. Rizomes of Curcuma longa, garlic and ginger, cloves and seeds of Garcina kola were extracted with water, methanol and ethanol sequentially and reconstituted with dimethyl -sulfoxide to concentrations (mg/ml) of 200, 100, 50, 25, 12.5, 6.25, 3.125 and 1.56. Mycoplasma isolates were screened for sensitivity to the extracts using agar well diffusion and broth diffusion methods. Mycoplasma hominis occurred more in women at (81.6%) while Ureaplasma Spp occurred more in men at (88%). Mycoplasma hominis (98%) and Ureaplasma Spp (91.6%) showed high sensitivityto ciprofloxacin. The test plants all showed presence of alkaloids, tannis, flavonoids, terpenoids, saponins, phenols and cardiac glycosides. At 200mg/ml Garcina kola and Curcurma longa showed higher zones of inhibition at 35mm on Mycoplasma hominis and ureaplasma Spp. Syngestic activities of ethanolic extracts of Curcuma longa and garlic showed high zones of inhibition at 45mm on Mycoplasma hominis while at 200mg/ml Garcina kola and cloves showed zones of inhibition at 45mm on ureaplasma spp. The minimum inhibitory concentration (MIC) results of the syngestic plants were 1.56mg/ml on all the isolates. The Results from the study showed that there is high prevalence of Mycoplasma hominis in women while Ureaplasma Spp while Ureaplasma Spp occurred more in men in the study area. The synergistic activities of selected edible plants showed higher efficacy on resistant isolates and suggest its use in the treatment of genital Mycoplasmas.

Published in	American Journal of Biomedical and Life Sciences (Volume 10, Issue 2)
DOI	10.11648/j.ajbls.20221002.14
Page(s)	28-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Genital Mycoplasmas, Syngestic Activities, Phytochemical, Edible Plant, Antibiotics

References

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Mbah-Omeje Kelechi, Ugwu Celestina Chibuzor. (2022). Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria. American Journal of Biomedical and Life Sciences, 10(2), 28-35. https://doi.org/10.11648/j.ajbls.20221002.14

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Mbah-Omeje Kelechi; Ugwu Celestina Chibuzor. Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria. Am. J. Biomed. Life Sci. 2022, 10(2), 28-35. doi: 10.11648/j.ajbls.20221002.14

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Mbah-Omeje Kelechi, Ugwu Celestina Chibuzor. Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria. Am J Biomed Life Sci. 2022;10(2):28-35. doi: 10.11648/j.ajbls.20221002.14

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@article{10.11648/j.ajbls.20221002.14,
  author = {Mbah-Omeje Kelechi and Ugwu Celestina Chibuzor},
  title = {Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {10},
  number = {2},
  pages = {28-35},
  doi = {10.11648/j.ajbls.20221002.14},
  url = {https://doi.org/10.11648/j.ajbls.20221002.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221002.14},
  abstract = {The prevalence rates of Mycoplasma hominis and Ureaplasma Spp and the evaluation of edible herbs against genital mycoplasmas were determined among university students in Enugu state, Nigeria. Specimens from 2400 subjects comprising of 1200 male and female subjects were tested for Mycoplasma hominis and Ureaplasma Spp. Cultures were done on Mycoplasma agar, A7agar and urea-arginine LYO2 broth. Antimicrobial susceptibility pattern was determined by Mycoplasma IST2 kit. Preliminary qualitative phytochemical analysis was done using standard methods to reveal the presence of basic phytochemicals. Rizomes of Curcuma longa, garlic and ginger, cloves and seeds of Garcina kola were extracted with water, methanol and ethanol sequentially and reconstituted with dimethyl -sulfoxide to concentrations (mg/ml) of 200, 100, 50, 25, 12.5, 6.25, 3.125 and 1.56. Mycoplasma isolates were screened for sensitivity to the extracts using agar well diffusion and broth diffusion methods. Mycoplasma hominis occurred more in women at (81.6%) while Ureaplasma Spp occurred more in men at (88%). Mycoplasma hominis (98%) and Ureaplasma Spp (91.6%) showed high sensitivityto ciprofloxacin. The test plants all showed presence of alkaloids, tannis, flavonoids, terpenoids, saponins, phenols and cardiac glycosides. At 200mg/ml Garcina kola and Curcurma longa showed higher zones of inhibition at 35mm on Mycoplasma hominis and ureaplasma Spp. Syngestic activities of ethanolic extracts of Curcuma longa and garlic showed high zones of inhibition at 45mm on Mycoplasma hominis while at 200mg/ml Garcina kola and cloves showed zones of inhibition at 45mm on ureaplasma spp. The minimum inhibitory concentration (MIC) results of the syngestic plants were 1.56mg/ml on all the isolates. The Results from the study showed that there is high prevalence of Mycoplasma hominis in women while Ureaplasma Spp while Ureaplasma Spp occurred more in men in the study area. The synergistic activities of selected edible plants showed higher efficacy on resistant isolates and suggest its use in the treatment of genital Mycoplasmas.},
 year = {2022}
}

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TY - JOUR
T1 - Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria
AU - Mbah-Omeje Kelechi
AU - Ugwu Celestina Chibuzor
Y1 - 2022/03/23
PY - 2022
N1 - https://doi.org/10.11648/j.ajbls.20221002.14
DO - 10.11648/j.ajbls.20221002.14
T2 - American Journal of Biomedical and Life Sciences
JF - American Journal of Biomedical and Life Sciences
JO - American Journal of Biomedical and Life Sciences
SP - 28
EP - 35
PB - Science Publishing Group
SN - 2330-880X
UR - https://doi.org/10.11648/j.ajbls.20221002.14
AB - The prevalence rates of Mycoplasma hominis and Ureaplasma Spp and the evaluation of edible herbs against genital mycoplasmas were determined among university students in Enugu state, Nigeria. Specimens from 2400 subjects comprising of 1200 male and female subjects were tested for Mycoplasma hominis and Ureaplasma Spp. Cultures were done on Mycoplasma agar, A7agar and urea-arginine LYO2 broth. Antimicrobial susceptibility pattern was determined by Mycoplasma IST2 kit. Preliminary qualitative phytochemical analysis was done using standard methods to reveal the presence of basic phytochemicals. Rizomes of Curcuma longa, garlic and ginger, cloves and seeds of Garcina kola were extracted with water, methanol and ethanol sequentially and reconstituted with dimethyl -sulfoxide to concentrations (mg/ml) of 200, 100, 50, 25, 12.5, 6.25, 3.125 and 1.56. Mycoplasma isolates were screened for sensitivity to the extracts using agar well diffusion and broth diffusion methods. Mycoplasma hominis occurred more in women at (81.6%) while Ureaplasma Spp occurred more in men at (88%). Mycoplasma hominis (98%) and Ureaplasma Spp (91.6%) showed high sensitivityto ciprofloxacin. The test plants all showed presence of alkaloids, tannis, flavonoids, terpenoids, saponins, phenols and cardiac glycosides. At 200mg/ml Garcina kola and Curcurma longa showed higher zones of inhibition at 35mm on Mycoplasma hominis and ureaplasma Spp. Syngestic activities of ethanolic extracts of Curcuma longa and garlic showed high zones of inhibition at 45mm on Mycoplasma hominis while at 200mg/ml Garcina kola and cloves showed zones of inhibition at 45mm on ureaplasma spp. The minimum inhibitory concentration (MIC) results of the syngestic plants were 1.56mg/ml on all the isolates. The Results from the study showed that there is high prevalence of Mycoplasma hominis in women while Ureaplasma Spp while Ureaplasma Spp occurred more in men in the study area. The synergistic activities of selected edible plants showed higher efficacy on resistant isolates and suggest its use in the treatment of genital Mycoplasmas.
VL - 10
IS - 2
ER -

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Author Information

Mbah-Omeje Kelechi

Department of Applied Microbiology and Brewing, Enugu State University of Science and Technology, Enugu, Nigeria
Ugwu Celestina Chibuzor

Department of Applied Microbiology and Brewing, Enugu State University of Science and Technology, Enugu, Nigeria

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Mbah-Omeje Kelechi, Ugwu Celestina Chibuzor. (2022). Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria. American Journal of Biomedical and Life Sciences, 10(2), 28-35. https://doi.org/10.11648/j.ajbls.20221002.14

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ACS Style

Mbah-Omeje Kelechi; Ugwu Celestina Chibuzor. Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria. Am. J. Biomed. Life Sci. 2022, 10(2), 28-35. doi: 10.11648/j.ajbls.20221002.14

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AMA Style

Mbah-Omeje Kelechi, Ugwu Celestina Chibuzor. Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria. Am J Biomed Life Sci. 2022;10(2):28-35. doi: 10.11648/j.ajbls.20221002.14

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@article{10.11648/j.ajbls.20221002.14,
  author = {Mbah-Omeje Kelechi and Ugwu Celestina Chibuzor},
  title = {Evaluation of Antibacterial Activity of Selected Edible Herbs Against Genital Mycoplasmas Among University Students in Enugu State, Nigeria},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {10},
  number = {2},
  pages = {28-35},
  doi = {10.11648/j.ajbls.20221002.14},
  url = {https://doi.org/10.11648/j.ajbls.20221002.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221002.14},
  abstract = {The prevalence rates of Mycoplasma hominis and Ureaplasma Spp and the evaluation of edible herbs against genital mycoplasmas were determined among university students in Enugu state, Nigeria. Specimens from 2400 subjects comprising of 1200 male and female subjects were tested for Mycoplasma hominis and Ureaplasma Spp. Cultures were done on Mycoplasma agar, A7agar and urea-arginine LYO2 broth. Antimicrobial susceptibility pattern was determined by Mycoplasma IST2 kit. Preliminary qualitative phytochemical analysis was done using standard methods to reveal the presence of basic phytochemicals. Rizomes of Curcuma longa, garlic and ginger, cloves and seeds of Garcina kola were extracted with water, methanol and ethanol sequentially and reconstituted with dimethyl -sulfoxide to concentrations (mg/ml) of 200, 100, 50, 25, 12.5, 6.25, 3.125 and 1.56. Mycoplasma isolates were screened for sensitivity to the extracts using agar well diffusion and broth diffusion methods. Mycoplasma hominis occurred more in women at (81.6%) while Ureaplasma Spp occurred more in men at (88%). Mycoplasma hominis (98%) and Ureaplasma Spp (91.6%) showed high sensitivityto ciprofloxacin. The test plants all showed presence of alkaloids, tannis, flavonoids, terpenoids, saponins, phenols and cardiac glycosides. At 200mg/ml Garcina kola and Curcurma longa showed higher zones of inhibition at 35mm on Mycoplasma hominis and ureaplasma Spp. Syngestic activities of ethanolic extracts of Curcuma longa and garlic showed high zones of inhibition at 45mm on Mycoplasma hominis while at 200mg/ml Garcina kola and cloves showed zones of inhibition at 45mm on ureaplasma spp. The minimum inhibitory concentration (MIC) results of the syngestic plants were 1.56mg/ml on all the isolates. The Results from the study showed that there is high prevalence of Mycoplasma hominis in women while Ureaplasma Spp while Ureaplasma Spp occurred more in men in the study area. The synergistic activities of selected edible plants showed higher efficacy on resistant isolates and suggest its use in the treatment of genital Mycoplasmas.},
 year = {2022}
}

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