Microorganisms are present on all surfaces, to which they were carried by many direct and indirect methods of transmission. Bacteria and Parasites contaminating office door handles in Nnamdi Azikiwe University were assessed between June and November, 2019. The buildings and the door handles were randomly selected. Sterile swabs moistened in sterile normal saline were used to swab the door handles in a tri-directional manner. The swabs were cultured and subcultured onto nutrient, MacConkey and Salmonella-Shigella agar plates for bacterial growth. The specimens were then examined for parasites using Zinc Sulphate floatation and formol-ether sedimentation techniques. Of the 432 door handles examined, 136 (30.8%) were contaminated. The highest number of contaminated doors were found in Medical Centre 41 (30.1%) and the least in Faculty of Social Sciences Complex 10 (7.4%). E. histolytica 52 (38.2%) was the highest parasite encountered while E. vermicularis 2 (1.5%) was the least. The proportion of infected doors as well as the distribution of different species parasites in the door handles of different complexes examined was significant (P<0.05). Of 432 office door handles examined, 263 (60.9%) yielded bacterial growth. Female hostel 72 (27.4%) had more bacterial contamination while Faculty of Social Sciences 25 (9.5%) had the least. Staphylococcus spp 54 (20.5%) was the highest bacteria isolated, followed by Escherichia 53 (20.2%) while Klebsiella 53 (20.2%) was the least. The distribution of different species of bacteria in the door handles in different complexes was significant (P<0.05). Infectious diseases from microbial contaminated door handles are a potential threat to public health and safety therefore certain measures should be taken such as aggressive and frequent cleaning methods and good hand washing to reduce the incidence of bacterial and parasite transmission and contamination.
| Published in | American Journal of Biomedical and Life Sciences (Volume 9, Issue 2) |
| DOI | 10.11648/j.ajbls.20210902.13 |
| Page(s) | 120-127 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Bacteria, Parasites, Contamination, Door Handles
| [1] | Eltablawy S. Y. and Elhinfnawi H. N. (2009). Microbial Contamination of Some Computer Keyboards and Mice in National Center for Radiation Research and Technology (NCRRT). World Applied Sciences Journal 6 (2): 162-167. |
| [2] | Apichai, S., Boonchai, W., Chittima, P., Oralak, S., Fukuda, C. D., Ladaporn, B. and Mason, C. J. (2005). Re-evaluation of commercially Available Enzyme-Linked immunosorbent assay for the detection of Giardia lamblia and Cryptosporidium spp. from stool specimens. Southeast Asian Journal of Tropical Medicine and Public Health, 36 (4): 26-29. |
| [3] | Kraay A. N. M., Michael A. L. Hayashi M. A. L., Hernandez-Ceron N., Spicknall I. H., Eisenberg M. C., Meza R and Eisenberg J. N. S (2018). Fomite-mediated transmission as a sufficient pathway: a comparative analysis across three viral pathogens. BMC Infectious Diseases 18: 540. |
| [4] | Neely A. and Sittig D. F. (2002). Basic microbiologic and infection control information to reduce the potential transmission of pathogens to patients via computer hardware. Journal of the American Medical Informatics Association 9 (5): 500-508. |
| [5] | Odigie A. B., Ekhiase F. O., Orjiakor P. I. and Omozuwa S. (2017). The role of door handles in the spread of microorganisms of public health consequences in university of Benin teaching hospital (UBTH), Benin City, Edo State. Pharmaceutical Science and Technology 2 (2): 15-21. |
| [6] | Li S., Eiseberg J. N. S., Sicknall I. H. and Koopman J. S. (2009): Dynamics and control of infections transmitted from person to person through the environment. American Journal of Epidemiology, 170 (2): 257-265. |
| [7] | Eiref S. D, Leitman I. M. and Riley W. (2012). Hand Sanitizer Dispensers and associated Hospital-acquired infections: Friend or Formite? Surgical Infections 13 (3): 137-140. |
| [8] | Lopez G. U., Gerba C. P., Tamimi A. H., Kitajima M., Maxwell S. L. and Rose J. B. (2013). Transfer efficiency of bacteria and viruses from porous and nonporous fomites to fingers under different relative humidity conditions. Applied and Environmental Microbiology 79, 5728-34 (2013). |
| [9] | Osterholm M. T., Hederg C. W. and MacDonald K. L. (1995): Epidemiology of infectious diseases. In: Mandell, Douglas and Bennett’s principles and Practice of Infectious diseases Vol. I, 4th edition. Churchill-Livingstone, New York. P. 165. |
| [10] | Bright KR, Boone SA, Gerba CP. (2010): Occurrence of bacteria and viruses on Elementary classroom surfaces and the potential role of Elementary Classroom hygiene in the spread of infectious diseases. The Journal of School Nursing, 26 (1): 33-41. |
| [11] | Boone, S. A. and Gerba, C. P. (2010): The prevalence of human Para-influenza virus on indoor office fomites. Food and Environmental Virology 2 (1): 41-46. |
| [12] | Shiferaw T., Beyene G., Kassa T. and Sewunet T. (2013). Bacterial contamination, Bacterial Profile and Antimicrobial Susceptibility Pattern of Isolates from Stethoscopes at Jimma University Specialized Hospital. Annals of Clinical Microbiology and Antimicrobials: 12: 39. |
| [13] | Schmidt M. G., Attaway H. H., Sharpe P. A., John J., Sepkowitz K. A. and Morgan A. (2012). Sustained reduction of microbial burden on common hospital surfaces through introduction of copper. Journal of Clinical Microbiology 50: 2217–2223. |
| [14] | Kramer A., Schwebke I. and Kampf G. (2006). How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infectious Diseases 6: 130. |
| [15] | Carter M. J. (2005). Enterically infecting viruses: Pathogenicity, Transmission and Significance for food and water borne infections. Journal of Applied Microbiology 98: 1354–1380. |
| [16] | Reynolds K. A. (2005): Hygiene of Environmental Surfaces. International Journal of Environmental Health Research, 15 (3): 225-234. |
| [17] | Rusin, P., Maxwell, S. and Gerba, C. (2002). Comparative surface to hand and fingertip to-mouth transfer efficiency of gram-positive bacterial, gram- negative bacterial and phage. Journal of Applied Microbiology, 93: 585-592. |
| [18] | Nworie. A., Ayeni. J. A., Eze U. A., and Azi. S. O. (2012). Bacterial contamination of door handles/knobs in selected public conveniences in abuja metropolis, nigeria; a public health threat. Continental Journal of Medical Research 6 (1): 7-11. |
| [19] | Tiku, D. R., Bassey, I. U. and Asikong, E. B. E (2019). Evaluation of Public Health Hazards of Door Handles in University of Calabar Community. International Journal of Scientific and Engineering Research 10 (Issue 6): 39-71. |
| [20] | Tanner, D. (2009). Reduction in infection risk through treatment of microbially contaminated suffices with a novel, portable, saturated steam vapor disinfection system. American Journal of Infection Control, 37: 20-27. |
| [21] | Clauditz, A., Resch, A., Wieland, K. P., Peschel, A. and Gotz, F. (2006). Staphyloxantin plays a roles in the fitness of Staphylococcus aureus and its ability to cope with oxidative stress. Infection and Immunity 74 (8), 4950-4953. |
| [22] | Aiello A. E., Marshall B., Levy, S, B., Della-Latta P and Larson E. (2004). Relationship between Triclosan and susceptibilities of bacteria isolated from hands in the community. Antimicrobial Agents of Chemotherapy. 48 (8): 2973-2979. |
| [23] | Chen, Y., Jackson, K. M., Chea, F. P. and Schaffner, D. W. (2001): Quantification and variability analysis of bacterial cross- contamination rates of common food service tasks. Journal of Food Protection, 64: 72-80. |
| [24] | Roberts, L. and Janovy, J. (2009). Foundations of Parasitology. New York: McGraw Hill. |
| [25] | Noble, E., Noble, G. A. Schad, G. A. and MacInnes, A. J. (1989). Parasitology. Philadelphia: Lea and Febiger. Nock I. H and Geneve A. I (2002): Public health significance of parasite cysts and egg on water closet handles. The Nigeria Journal of Parasitology 17: 1-94. |
| [26] | Current, W. L. and Garcia, L. S. (1991). Cryptosporidiosis, Clinical Microbiology Reviews, 4: 325–358. |
| [27] | World Health Organization (1997). Amoebiasis. WHO Weekly Epidemiologic Records, 72: 97-100. |
| [28] | Akogun O. B. (1989): Some social aspects of helminthiasis among the people of Gumaru district, Bauchi State. Nigeria Journal of Tropical Medicine and Hygiene; 92 (3): 193-196. |
| [29] | Ngoda F. (2017). Assessment of Bacterial contamination of toilet and bathroom door handles/knobs at Daeyang Luke Hospital. Pharmaceutical and Biological Evaluations 4 (4): 193-197. |
| [30] | Sattar S. A. (2001). Survival of microorganisms on animate and inanimate surfaces and their disinfection. In Rutala WA, editor, Disinfection, Sterilization and Antisepsis: principles and practices in healthcare facilities. Association for Professionals in Infection Control and Epidemiology, Inc., Washington D. C: 195-205. |
| [31] | Oranusi S. U., Dahunsi S. O., Owoso O. O. and Olatile T. (2013). Microbial profiles of Hands, Foods, Easy contact surfaces and Food contact surfaces: A case study of a University Campus. Novus International Journal of Biotechnology and Bioscience 2 (1): 30-38. |
| [32] | Cheesbrough, M. (2006a). Parasitological Tests. District Laboratory Practice in Tropical Countries Part 1. Cambridge Low-Price Edition, Cambridge University Press, United Kingdom: 1-258. |
| [33] | Cheesbrough, M. (2006b). Microbiological tests, District Laboratory Practice in Tropical Countries Part 2, Second Edition, Cambridge Low-Price Edition, Cambridge University Press, United Kingdom: 1-154. |
| [34] | Holt J. G., Krieg N. R., Sneath P. H., Staley J. T. and Williams S. T. (1994). Bergey’s manual of Determinative Bacteriology, 9th edition. Baltimore, Md: The Williams & Wilkins Co, Pp 787. |
| [35] | Ndams, I. S. and Jimoh, O. I. (2006). Prevalence of Parasite Eggs and Cysts on Computer Mouse. Keyboards and Users of Internet Cafes, in Samaru, Zaria, Nigeria. The Zoologist, 1 (4): 80-85. |
| [36] | Orji, N., Esiaka, E., Anyaegbunam, L., Obi, R. and Ezeagwuna, D. (2012). Parasite Contamination of Nigerian Currency (Paper and Polymer Notes) in Ihiala Local Government Area of Anambra State, Nigeria. The Internet Journal of Infectious Diseases, 10 (1): 20-25. |
| [37] | Aminu M., Usman-Sani H. and Usman M. A. (2014). Characterization and determination of antibiotic susceptibility pattern of bacteria isolated from some fomites in a Teaching Hospital in Northern Nigeria. African Journal of Microbiology Research 8 (8): 814-818. |
| [38] | Ajero, C. M., Nwoko, B. E. B., Nwoke, E. A. and Ukaga, C. N. (2008). Human Amoebiasis: Distribution and Burden; and the Nigerian environment. International Science Research Journal, 1 (2): 130-134. |
| [39] | Ibidapo, C. A. and Okwa, O. (2008). The prevalence and intensity of soil transmitted helminthes in a rural community, Lagos suburb, south west Nigeria. International Journal of Agriculture and Biology, 10: 89–92. |
| [40] | Centers for Disease Control and Prevention - CDC (2010). Cryptosporidiosis surveillance and Giardiasis Surveillance. Surveillance Summaries, United States, 2006–2008. Morbidity and Mortality Weekly Report, 59: (No. SS-#). |
| [41] | Gaspard, P., Wiart, J. and Schwartzbrod, J. (1997). Parasitological contamination of urban sludge used for agricultural purposes. Waste Management Research 15: 429-436. |
| [42] | Umeanaeto, P. U, Nwofor, O. F, Iwueze, M. O., Ugwuanyi, I. K., Egbuche, C. M. and Igbodika, C. M. (2016). Parasite Contamination of Edible Vegetables sold in Onitsha Markets. The Bioscientist 4 (1): 21-31. |
| [43] | Abiose O. F. (2019). Bacterial Contamination of Selected Public Toilet Door Handles within Adekunle Ajasin University Campus, Akungba-Akoko, Ondo State, Nigeria. International Journal of Sciences: Basic and Applied Research (IJSBAR) 43 (1): 76-86. |
| [44] | Al-Harbi M., Anderson A., Elmi A. (2017). Evaluation of microbial contamination in frequently used Fomites in Kuwait. Biodiversity International Journal 1 (Issue 3): 80-86. |
| [45] | Hisham A. A. S. (2015). Assessment of Gram-Negative Bacterial Contamination on Door Handles of Service Offices in Sudanese Universities, Khartoum State. A dissertation submitted in partial fulfillment for the requirements of MSc in Medical Laboratory Science (Microbiology): 1-34. |
| [46] | Omololu-Aso, J., Kolawole, D. O., Omololu-Aso, O. O., and Ajisebutu, S O. (2011). Antibiotics sensitivity pattern of staphylococcus aureus from fomites in the ObafemiAwolowo University Teaching Hospital Complex (OAUTHC) Nigeria. International Journal of Medicine and Medical Sciences 3 (2): 32-36. |
| [47] | Ajenifuja, O. A. and Ajibade, V. A (2012). Prevalence of Parasitic Eggs and Parasites Cysts on Computer Mouse and Keyboard in School of Science and Computer Studies of Federal Polytechnic, Ado-Ekiti, Nigeria. Global Journal of Science Frontier Research Bio-Tech and Genetics 12 (issue 4) version 1: 26-30. |
| [48] | Alonge, O. O; Auwal B. M, Aboh M. I. (2019). Bacterial Contamination of Toilet door handles on Baze University Campus Abuja, Nigeria. African Journal of Clinical and Experimental Microbiology 20 (1): 35-41. |
| [49] | Lincy M., Vidhya, Arunadevi, Ali P. S. S. and John J (2016). Determination of bacterial and fungal loads and antibiotic susceptibility testing of bacteria isolated from public toilet door handles in Vellore district, Tamilnadu, India. International Journal of Research in Biosciences 5 (Issue 4): 69-78. |
| [50] | Bhatta D. R, Hamal D, Shrestha R, Subramanya SH, Baral N, Singh RK, Nayak N, and Gokhale S (2018). Bacterial contamination of frequently touched objects in a tertiary care hospital of Pokhara, Nepal: how safe are our hands? Antimicrobial Resistance and Infection Control. 7: 97. |
| [51] | Baker J. and Bloomfield S. F. (2000). Survival of Salmonella in bathroom and toilets in homes. Journal of Applied Microbiology 89: 137-44. |
| [52] | Maori L., Agbor V. O. and Ahmed W. A. (2013). The prevalence of bacterial organisms on toilet door handles in secondary schools in Bokkos L. G. A., Jos, Plateau Sate, Nigeria. Journal of Pharmaceutical and Biological Sciences 8 (4): 85-91. |
| [53] | Lindberg E., Adlerberth B., Hesselmar R., Saalman I., Strannegared N. and Aberg A. (2004). High rate of transfer of Staphylococcus aureus from parental skin to infant gut flora. Journal of Clinical Microbiology 42: 530-534. |
| [54] | Flores G. E., Bates S. T., Knights D., Lauber C. L., Stombaugh J., Knight R. and Fierer N. (2011). Microbial Biogeography of Public Restroom Surfaces. PLoS ONE 6 (11): e28132. |
| [55] | Alonge O. O., Wakkala F. I., Ogbaga C. C., Akindele K. A. (2017). Bacterial analysis of barbecued meat (Suya) from selected locations within Abuja, Nigeria. Proceedings of the 13th International Conference on Electronics, Computer and Computation (ICECCO), Abuja, Nigeria. pp. 1-5. |
| [56] | Willey J. M., Sherwood LM, Woolverton C. J. (2008). Prescott, Harley, and Klein’s Microbiology, 7th edition. McGraw Hill Company Incorporated. New York. pp. 1088. |
| [57] | Baadhaim M., Kusner, D. and Ahmed H. (2011). Distribution and Prevalence of Bacteria Found on the Door Handles of Olin Hall, Drake University. Conference Poster. |
APA Style
Pauline Ukamaka Umeanaeto, Ugochukwu Chukwuma Okafor, Miracle Chisom Unam, Chidiogo Chidimma Ilo, Chukwudinma Chigozie Okoli, et al. (2021). Assessment of Parasites and Bacterial Contamination of Office Door Handles in Nnamdi Azikiwe University, Awka, Anambra State. American Journal of Biomedical and Life Sciences, 9(2), 120-127. https://doi.org/10.11648/j.ajbls.20210902.13
ACS Style
Pauline Ukamaka Umeanaeto; Ugochukwu Chukwuma Okafor; Miracle Chisom Unam; Chidiogo Chidimma Ilo; Chukwudinma Chigozie Okoli, et al. Assessment of Parasites and Bacterial Contamination of Office Door Handles in Nnamdi Azikiwe University, Awka, Anambra State. Am. J. Biomed. Life Sci. 2021, 9(2), 120-127. doi: 10.11648/j.ajbls.20210902.13
AMA Style
Pauline Ukamaka Umeanaeto, Ugochukwu Chukwuma Okafor, Miracle Chisom Unam, Chidiogo Chidimma Ilo, Chukwudinma Chigozie Okoli, et al. Assessment of Parasites and Bacterial Contamination of Office Door Handles in Nnamdi Azikiwe University, Awka, Anambra State. Am J Biomed Life Sci. 2021;9(2):120-127. doi: 10.11648/j.ajbls.20210902.13
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Download@article{10.11648/j.ajbls.20210902.13,
author = {Pauline Ukamaka Umeanaeto and Ugochukwu Chukwuma Okafor and Miracle Chisom Unam and Chidiogo Chidimma Ilo and Chukwudinma Chigozie Okoli and Stella Chinyere Afulukwe and Nnamdi Enoch Nwakoby},
title = {Assessment of Parasites and Bacterial Contamination of Office Door Handles in Nnamdi Azikiwe University, Awka, Anambra State},
journal = {American Journal of Biomedical and Life Sciences},
volume = {9},
number = {2},
pages = {120-127},
doi = {10.11648/j.ajbls.20210902.13},
url = {https://doi.org/10.11648/j.ajbls.20210902.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210902.13},
abstract = {Microorganisms are present on all surfaces, to which they were carried by many direct and indirect methods of transmission. Bacteria and Parasites contaminating office door handles in Nnamdi Azikiwe University were assessed between June and November, 2019. The buildings and the door handles were randomly selected. Sterile swabs moistened in sterile normal saline were used to swab the door handles in a tri-directional manner. The swabs were cultured and subcultured onto nutrient, MacConkey and Salmonella-Shigella agar plates for bacterial growth. The specimens were then examined for parasites using Zinc Sulphate floatation and formol-ether sedimentation techniques. Of the 432 door handles examined, 136 (30.8%) were contaminated. The highest number of contaminated doors were found in Medical Centre 41 (30.1%) and the least in Faculty of Social Sciences Complex 10 (7.4%). E. histolytica 52 (38.2%) was the highest parasite encountered while E. vermicularis 2 (1.5%) was the least. The proportion of infected doors as well as the distribution of different species parasites in the door handles of different complexes examined was significant (PStaphylococcus spp 54 (20.5%) was the highest bacteria isolated, followed by Escherichia 53 (20.2%) while Klebsiella 53 (20.2%) was the least. The distribution of different species of bacteria in the door handles in different complexes was significant (P<0.05). Infectious diseases from microbial contaminated door handles are a potential threat to public health and safety therefore certain measures should be taken such as aggressive and frequent cleaning methods and good hand washing to reduce the incidence of bacterial and parasite transmission and contamination.},
year = {2021}
}
TY - JOUR T1 - Assessment of Parasites and Bacterial Contamination of Office Door Handles in Nnamdi Azikiwe University, Awka, Anambra State AU - Pauline Ukamaka Umeanaeto AU - Ugochukwu Chukwuma Okafor AU - Miracle Chisom Unam AU - Chidiogo Chidimma Ilo AU - Chukwudinma Chigozie Okoli AU - Stella Chinyere Afulukwe AU - Nnamdi Enoch Nwakoby Y1 - 2021/04/29 PY - 2021 N1 - https://doi.org/10.11648/j.ajbls.20210902.13 DO - 10.11648/j.ajbls.20210902.13 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 120 EP - 127 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20210902.13 AB - Microorganisms are present on all surfaces, to which they were carried by many direct and indirect methods of transmission. Bacteria and Parasites contaminating office door handles in Nnamdi Azikiwe University were assessed between June and November, 2019. The buildings and the door handles were randomly selected. Sterile swabs moistened in sterile normal saline were used to swab the door handles in a tri-directional manner. The swabs were cultured and subcultured onto nutrient, MacConkey and Salmonella-Shigella agar plates for bacterial growth. The specimens were then examined for parasites using Zinc Sulphate floatation and formol-ether sedimentation techniques. Of the 432 door handles examined, 136 (30.8%) were contaminated. The highest number of contaminated doors were found in Medical Centre 41 (30.1%) and the least in Faculty of Social Sciences Complex 10 (7.4%). E. histolytica 52 (38.2%) was the highest parasite encountered while E. vermicularis 2 (1.5%) was the least. The proportion of infected doors as well as the distribution of different species parasites in the door handles of different complexes examined was significant (PStaphylococcus spp 54 (20.5%) was the highest bacteria isolated, followed by Escherichia 53 (20.2%) while Klebsiella 53 (20.2%) was the least. The distribution of different species of bacteria in the door handles in different complexes was significant (P<0.05). Infectious diseases from microbial contaminated door handles are a potential threat to public health and safety therefore certain measures should be taken such as aggressive and frequent cleaning methods and good hand washing to reduce the incidence of bacterial and parasite transmission and contamination. VL - 9 IS - 2 ER -
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