Protective and Curative Potentials of <i>Sida corymbosa</i> Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride

Charles Chijioke Dike; Francis Chukwuemeka Ezeonu; Chinenye Enoch Oguazu; Emmanuel Nonso Ezeokafor; Hugh Clifford Chima Maduka; Aloysius Ngozika Okpogba; Akpoghene Eyeghre Onoriode; Sunday Jacob Asebioyo; Emilia Chika Nwankwo; Ebelechukwu Nwaoma Ihekwoaba; Aronu Cecilia Nkechi

doi:doi:10.11648/j.ajbls.20210902.11

Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, Aloysius Ngozika Okpogba, Akpoghene Eyeghre Onoriode, Sunday Jacob Asebioyo, Emilia Chika Nwankwo, Ebelechukwu Nwaoma Ihekwoaba, Aronu Cecilia Nkechi

Published in American Journal of Biomedical and Life Sciences (Volume 9, Issue 2)

Received: 27 May 2020 Accepted: 15 June 2020 Published: 4 March 2021

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Abstract

Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl₄). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A₁-H₄) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl₄ only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl₄ administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (P<0.05) in the treatment groups from 7-28 d, when compared with the negative control. Oxidative assay carried out showed that serum malondialdehyde (MDA) levels were significantly lower (P<0.05) in treatment groups than in negative control groups. These may be suggesting that the extract may have improved the antioxidant levels of the rats. Sida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl₄.

Published in	American Journal of Biomedical and Life Sciences (Volume 9, Issue 2)
DOI	10.11648/j.ajbls.20210902.11
Page(s)	97-110
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Sida Species, Antioxidant Results in Rats, Oxidative Stress

References

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APA Style

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. (2021). Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. American Journal of Biomedical and Life Sciences, 9(2), 97-110. https://doi.org/10.11648/j.ajbls.20210902.11

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ACS Style

Charles Chijioke Dike; Francis Chukwuemeka Ezeonu; Chinenye Enoch Oguazu; Emmanuel Nonso Ezeokafor; Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am. J. Biomed. Life Sci. 2021, 9(2), 97-110. doi: 10.11648/j.ajbls.20210902.11

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AMA Style

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am J Biomed Life Sci. 2021;9(2):97-110. doi: 10.11648/j.ajbls.20210902.11

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@article{10.11648/j.ajbls.20210902.11,
  author = {Charles Chijioke Dike and Francis Chukwuemeka Ezeonu and Chinenye Enoch Oguazu and Emmanuel Nonso Ezeokafor and Hugh Clifford Chima Maduka and Aloysius Ngozika Okpogba and Akpoghene Eyeghre Onoriode and Sunday Jacob Asebioyo and Emilia Chika Nwankwo and Ebelechukwu Nwaoma Ihekwoaba and Aronu Cecilia Nkechi},
  title = {Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {9},
  number = {2},
  pages = {97-110},
  doi = {10.11648/j.ajbls.20210902.11},
  url = {https://doi.org/10.11648/j.ajbls.20210902.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210902.11},
  abstract = {Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.},
 year = {2021}
}

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TY  - JOUR
T1  - Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride
AU  - Charles Chijioke Dike
AU  - Francis Chukwuemeka Ezeonu
AU  - Chinenye Enoch Oguazu
AU  - Emmanuel Nonso Ezeokafor
AU  - Hugh Clifford Chima Maduka
AU  - Aloysius Ngozika Okpogba
AU  - Akpoghene Eyeghre Onoriode
AU  - Sunday Jacob Asebioyo
AU  - Emilia Chika Nwankwo
AU  - Ebelechukwu Nwaoma Ihekwoaba
AU  - Aronu Cecilia Nkechi
Y1  - 2021/03/04
PY  - 2021
N1  - https://doi.org/10.11648/j.ajbls.20210902.11
DO  - 10.11648/j.ajbls.20210902.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 97
EP  - 110
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20210902.11
AB  - Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.
VL  - 9
IS  - 2
ER  -

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Author Information

Charles Chijioke Dike

Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Francis Chukwuemeka Ezeonu

Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria
Chinenye Enoch Oguazu

Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria
Emmanuel Nonso Ezeokafor

Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Hugh Clifford Chima Maduka

Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Aloysius Ngozika Okpogba

Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Akpoghene Eyeghre Onoriode

Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Sunday Jacob Asebioyo

Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Emilia Chika Nwankwo

Department of Human Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Ebelechukwu Nwaoma Ihekwoaba

Department of Environmental Health Sciences, Faculty of Health Sciences and Technology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria
Aronu Cecilia Nkechi

Department of Environmental Health Sciences, Faculty of Health Sciences and Technology, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria

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Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. (2021). Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. American Journal of Biomedical and Life Sciences, 9(2), 97-110. https://doi.org/10.11648/j.ajbls.20210902.11

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Charles Chijioke Dike; Francis Chukwuemeka Ezeonu; Chinenye Enoch Oguazu; Emmanuel Nonso Ezeokafor; Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am. J. Biomed. Life Sci. 2021, 9(2), 97-110. doi: 10.11648/j.ajbls.20210902.11

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AMA Style

Charles Chijioke Dike, Francis Chukwuemeka Ezeonu, Chinenye Enoch Oguazu, Emmanuel Nonso Ezeokafor, Hugh Clifford Chima Maduka, et al. Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride. Am J Biomed Life Sci. 2021;9(2):97-110. doi: 10.11648/j.ajbls.20210902.11

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@article{10.11648/j.ajbls.20210902.11,
  author = {Charles Chijioke Dike and Francis Chukwuemeka Ezeonu and Chinenye Enoch Oguazu and Emmanuel Nonso Ezeokafor and Hugh Clifford Chima Maduka and Aloysius Ngozika Okpogba and Akpoghene Eyeghre Onoriode and Sunday Jacob Asebioyo and Emilia Chika Nwankwo and Ebelechukwu Nwaoma Ihekwoaba and Aronu Cecilia Nkechi},
  title = {Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {9},
  number = {2},
  pages = {97-110},
  doi = {10.11648/j.ajbls.20210902.11},
  url = {https://doi.org/10.11648/j.ajbls.20210902.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210902.11},
  abstract = {Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.},
 year = {2021}
}

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TY  - JOUR
T1  - Protective and Curative Potentials of Sida corymbosa Extract Against Antioxidant Depletion in Rats Administered Carbon Tetrachloride
AU  - Charles Chijioke Dike
AU  - Francis Chukwuemeka Ezeonu
AU  - Chinenye Enoch Oguazu
AU  - Emmanuel Nonso Ezeokafor
AU  - Hugh Clifford Chima Maduka
AU  - Aloysius Ngozika Okpogba
AU  - Akpoghene Eyeghre Onoriode
AU  - Sunday Jacob Asebioyo
AU  - Emilia Chika Nwankwo
AU  - Ebelechukwu Nwaoma Ihekwoaba
AU  - Aronu Cecilia Nkechi
Y1  - 2021/03/04
PY  - 2021
N1  - https://doi.org/10.11648/j.ajbls.20210902.11
DO  - 10.11648/j.ajbls.20210902.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 97
EP  - 110
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20210902.11
AB  - Sida corymbosa plant is one of medicinal plants used in many parts of Africa, including Nigeria for the treatment of human diseases. The purpose of this work was to investigate the antioxidant effects of Sida corymbosa ethanolic leaf extract on rats administered carbon tetrachloride (CCl4). One hundred and sixty male-rats between 170-180 g were used. They were grouped into 8 major groups (groups A-H) and 4 subgroups (A1-H4) in each group making it 32 subgroups of 5 rats each. The major groups comprise A (positive control- normal rats giving feed and water only and left for 7-28 d), B (negative control-those administered CCl4 only at 0.4 ml/kgbw and left for 7-28 d), C-H (treatment groups-those administered extract before and after CCl4 administration at 5,000, 3,000 and 1,000 mg/kgbw for 7-28 d). Antioxidant assays were determined using UV-VIS spectrophotometer (model 752g, China). Results of serum antioxidant assays done revealed that levels of serum reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), supraoxide dismutase (SOD) and total antioxidant capacity (TAC) increased significantly (PSida corymbosa ethanolic leaf extract may, therefore, have positive effects on antioxidant status of rats administered CCl4.
VL  - 9
IS  - 2
ER  -

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