Anthelmintic and Antimicrobial Activities of Tannin Extracts of <i>Mitragyna inermis </i>(Willd.) Kuntze (Rubiaceae) and <i>Combretum glutinosum </i>Perr. ex DC (Combretaceae)

Eléonore Chikani Ladekan-Yayi; Placide Mahougnan Toklo; Durand Dah-Nouvlessounon; Mahoudo Fidèle Assogba; Steven Collins Njonté Wouamba; Billy Toussie Tchegnitegni; Géorcelin Goué Alowanou; Lamine Baba-Moussa; Sylvie Hounzangbe-Adote; Bruno Ndjakou Lenta; Siméon Fogue Kouam; Gbenou Joachim Djimon

doi:doi:10.11648/j.ajac.20210905.14

Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae)

Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, Billy Toussie Tchegnitegni, Géorcelin Goué Alowanou, Lamine Baba-Moussa, Sylvie Hounzangbe-Adote, Bruno Ndjakou Lenta, Siméon Fogue Kouam, Gbenou Joachim Djimon

Published in American Journal of Applied Chemistry (Volume 9, Issue 5)

Received: 30 August 2021 Accepted: 15 September 2021 Published: 26 September 2021

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Abstract

Combretum glutinosum and Mitragyna inermis are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested in vitro on Haemonchus contortus larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on H. contortus larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, M. inermis extract had an effect only on Pseudomonas aeruginosa with a MIC of 2.5 mg/mL. C. glutinosum extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of C. glutinosum (IC₅₀ = 8.04 µg/mL) and M. inermis (IC₅₀ = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of C. glutinosum and could explain its activities. The results obtained from this work revealed that the tannins extracted from C. glutinosum showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from M. inermis.

Published in	American Journal of Applied Chemistry (Volume 9, Issue 5)
DOI	10.11648/j.ajac.20210905.14
Page(s)	145-153
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

M. inermis, C. glutinosum, Total Tannins, Anthelmintics, Antimicrobials, Antioxidants, LC-MS

References

[1]	Anthoula A. (2003). Plantes Aromatiques et Médicinales. Projet “Assistance au Recensement Agricole”. FAO.
[2]	Meziti A. (2009). Activité antioxydante des extraits des graines de Nigella sativa L. étude in vitro et in vivo. Master en Biochimie appliquée. Université El-haj Lakhdar Batna, pp. 105.
[3]	WHO. (2007). Stratégie de l’OMS pour la Médecine Traditionnelle pour 2002–2005. Organisation Mondiale de la Santé Genève.
[4]	Koné MW, Kamanzi AK. (2006). Inventaire ethnomédical et évaluation de l'activité anthelminthique des plantes médicinales utilisées en Côte d'ivoire contre les helminthiases intestinales. Pham. Méd. Trad. Afr, 10 (5): 55-72.
[5]	Dedehou VFGN. (2015). Utilisation de Parkia biglobosa et de Pterocarpus erinaceus dans la gestion du parasitisme à Haemonchus contortus chez le mouton Djallonké au Bénin. Thèse de doctorat es Science de la faculté des Sciences Agronomique de l’Université d’Abomey-Calavi, pp. 270.
[6]	Mangambu MJD, Mushagalusa KL, Kadima NJ. (2014). Contribution à l'étude phytochimique de quelques plantes médicinales antidiabétiques de la ville de Bukavu et ses environs (SudKivu, R. D. Congo). Journal of Applied Biosciences, 57: 6211 – 6220.
[7]	Adjanohoun E, Ahyi MRA, Ake AL, Dramane K, Elewude JA, Fadoju SO, Gbile ZO, Goudote E, Johnson CLA, Keita A, Morakinyo O, Ojewole JAO, Olatunjia AO, Sofowora EA. (1991). Contribution to ethnobotanical and floristic studies in Western Nigeria. CSTR-OUA, pp. 420.
[8]	Konkon NG, Ouatara D, Kpan WB and Kouakou TH. (2017). Medicinal plants used for treatment of diabetes by traditional practitioners in the markets of Abidjan district in Côte d'Ivoire. Journal of Medicinal Plants Studies, 5 (2): 39-48.
[9]	Kerharo J, Adam JG. (1974). La pharmacopée sénégalaise traditionnelle. Paris.
[10]	Attindéhou S, Houngnimassoun MA, Salifou S, Biaou CF. (2012). Inventorying of herbal remedies used to control small ruminant’s parasites in Southern Benin. Int. Multidisci. Res. J, 2 (8): 14-16.
[11]	Kabore A, Tamboura HH, Belem AMG, Traore A. (2007). Traitements ethno-vétérinaires des parasitoses digestives des petits ruminants dans le plateau central du Burkina Faso. Int. J. Biol. Chem. Sci, 1 (3): 297-304.
[12]	Feknous S, Saidi F, Mohamed SR. (2014). Extraction, caractérisation et identification de quelques métabolites secondaires actifs de la mélisse (Melissa officinalis L.). Nature et Technologie, 11: 7-13.
[13]	Houghton PJ, Raman A. (1998). Laboratory handbook for the fractionation of natural extracts. New York: Chapman and hall, pp. 208.
[14]	Rabel B, McGregor P, Dough G. (1994). Improved bioassay for estimation of effects of ovine gastrointestinal inhibitory mucus and on nematode larval migration anthelminthic. International Journal of Parasitology, 24: 671-676.
[15]	Toklo PM, Yayi E, Sakirigui A, Alowanou GG, Assogba MF, Kakpo B, Hounzangbé-Adoté S, Gbenou JD. (2019). Phytochemical screening and in vitro effect of watery triturated extract of Mitragyna inermis (Willd.) Kuntz (Rubiaceae) on Haemonchus contortus. Int. J. Adv. Res. 7 (12): 762-767.
[16]	Hounzangbe-Adote MS, Paolini V, Fouraste I, Moutairou K, Hoste H. (2005). In vitro effects of four tropical plants on three life-cycle stages of the parasitic nematode, Haemonchus contortus. Research in Veterinary Science, 78: 155–160.
[17]	Toklo PM, Ladekan-Yayi EC, Sakirigui A, Alowanou GG, Assogba MF, Challaton KP, Hounzangbe-Adote S, Gbenou JD. (2021). Antioxidant and antiparasitic activities on Trichostrongylus colubriformis of the aqueous extract of fresh leaves of Mitragyna inermis (Willd.) Kuntze (Rubiaceae). The Pharmaceutical and Chemical Journal. 8 (1): 120-128.
[18]	Bauer AW, Kirby WM, Sherris JC, Turck M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45: 493-496.
[19]	Delarras C. (1998). Microbiologie. 90 heures de travaux pratiques. Gaétan Morien Editeur, pp. 169-178.
[20]	Lamien-Meda A, Lamien CE, Compaore MMY, Meda RNT, Kiendrebeogo M. (2008). Polyphenol content and antioxidant activity of fourteen wild edible fruits from Burkina Faso. Molecules, 13: 581-594.
[21]	Hinneburg I, Dorman, HJD, Hiltunen R. (2006). Antioxidant activities of extracts from selected culinary herbs and spices. Food chemistry, 97, 122-129.
[22]	Bakasso S. (2009). Études phytochimiques et potentialités biologiques de cinq espèces d'indigofera (fabaceae) utilisées en médecine traditionnelle au Burkina Faso. Thèse de Doctorat. Université d’Ouagadougou, pp. 135.
[23]	Arbenz A, Avérous L. (2015). Chemical modification of tannins to elaborate aromatic biobased macromolecular architectures. Green Chem, 17, 2626–2646.
[24]	Adnyana IK, Tezuka Y, Awale S, Banskota AH, Tran KQ, Kadota S. (2001). 1-O-Galloyl-6-O-(4-hydroxy-3,5-dimethoxy)benzoyl-β-D-glucose, a New Hepatoprotective Constituent from Combretum quadrangulare. Planta Medica, 67 (4), 370–371.
[25]	Jossang A, Pousset JL, Bow B. (1994). Combreglutinin, a hydrolyzable tannin from Combretum glutlnosum. Journal of Natural Products, 57 (6): 732-737.
[26]	Benabdallah FZ, Kouamé RO, El Bentchikou M, Zellagui A, Gherraf N. (2015). Ethnobotanical and phytochemical Studies and assessment of antimicrobial activity of the leaves of pistachio oleoresin of the atlas (Pistacia atlantica Desf.). Phytothérapie. DOI 10.1007/s10298-015-0926-2.
[27]	Hoste H, Brunet S, Paolini V, Bahuaud D, Chauveau S, Fouraste I, Lefrileux Y. (2009). Compared in vitro anthelmintic effects of eight tannin-rich plants browsed by goats in the southern part of France. Nutritional and foraging ecology of sheep and goats. Zaragoza: CIHEAM / FAO / NAGREF, pp. 431 – 436.
[28]	Vauzour D. (2004). Étude des propriétés biologiques des constituants des vins de Champagne. Doctorat de l’Université Montpellier I, pp. 258.
[29]	Hadj Salem J. (2018). Extraction, identification, caractérisation des activités biologiques de flavonoïdes de Nitraria retusa et synthèse de dérivés acylés de ces molécules par voie enzymatique. Thèse de doctorat à l’Institut National Polytechnique de Lorraine, pp. 271.
[30]	Brunet S, Jackson F, Hoste H. (2008). Effects of sainfoin (Onobrychis viciifolia) extract and monomers of condensed tannins on the association of abomasal nematode larvae with fundic explants. Int. J. Parasitol, 38: 783-790.
[31]	Alowanou GG, Olounladé PA, Akouèdegni GC, Faihun AML, Koudandé DO, Hounzangbé-Adoté S. (2019). In vitro anthelmintic effects of Bridelia ferruginea, Combretum glutinosum, and Mitragyna inermis leaf extracts on Haemonchus contortus, an abomasal nematode of small ruminants. Helminthology. Parasitology Research. https://doi.org/10.1007/s00436-019-06262-5
[32]	Asase A, Kokubun T, Grayer RJ, Kite G, Simmonds MSJ, Oteng-Yeboah AA, Odamtten GT. (2008). Chemical constituents and antimicrobial activity of medicinal plants from Ghana: Cassia sieberiana, Haematostaphis barteri, Mitragyna inermis and Pseudocedrela kotschyi. Phytotherapy Research, 22 (8): 1013-16.
[33]	Zumbes HJ, Babalola OB, Nanle CD, Katnap RS, David EV, Anejo-Okopi AJ, Dabo DA, Gokir JD, Nvau JB. (2017). Antibacterial Potency of Combined Extracts of Mitragyna inermis (Linn) and Monotes kerstingii (Linn) on Salmonella Typhi and Salmonella Paratyphi A and its in vivo toxicity against Swiss Albino Mice. Journal of Microbiology Research, 2: 122-129.
[34]	Wakirwa JH, Yawate UE, Zakama SG, Muazu J, Madu SJ. (2013). Phytochemical and antibacterial screening of the methanol leaf extract of Mitragyna inermis (Wild o. Ktze Rubiaceae). International Journal of Pharmaceutical Research and Innovation, 6: 1-6.
[35]	Usman H, Sadiq FA, Mohammed B, Umar HA, Tijjani MA, Pindiga NY, Zadva AI, Thliza BA, Ahmed IA. (2017). Phytochemical and in vitro antimicrobial efficacies of the crude flavonoids and saponins rootbark extract of Combretum glutinosum perrot. Ex. Dc. Chemistry Research Journal, 2 (4): 31-36.
[36]	Yahaya O, Yabefa AJ, Usman B. (2012). Phytochemical Screening and Antibacterial Activity of Combretum glutinosum extract against Some Human Pathogens. British Journal of Pharmacology and Toxicology, 3 (5): 233-236.
[37]	Manallah A. (2012). Activités antioxydante et anticoagulante des polyphénols de la pulpe d’olive Olea europaea L. Master de l’Université Ferhat Abbas-Sétif, pp. 132.

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Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, et al. (2021). Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). American Journal of Applied Chemistry, 9(5), 145-153. https://doi.org/10.11648/j.ajac.20210905.14

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Eléonore Chikani Ladekan-Yayi; Placide Mahougnan Toklo; Durand Dah-Nouvlessounon; Mahoudo Fidèle Assogba; Steven Collins Njonté Wouamba, et al. Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). Am. J. Appl. Chem. 2021, 9(5), 145-153. doi: 10.11648/j.ajac.20210905.14

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AMA Style

Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, et al. Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). Am J Appl Chem. 2021;9(5):145-153. doi: 10.11648/j.ajac.20210905.14

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@article{10.11648/j.ajac.20210905.14,
  author = {Eléonore Chikani Ladekan-Yayi and Placide Mahougnan Toklo and Durand Dah-Nouvlessounon and Mahoudo Fidèle Assogba and Steven Collins Njonté Wouamba and Billy Toussie Tchegnitegni and Géorcelin Goué Alowanou and Lamine Baba-Moussa and Sylvie Hounzangbe-Adote and Bruno Ndjakou Lenta and Siméon Fogue Kouam and Gbenou Joachim Djimon},
  title = {Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae)},
  journal = {American Journal of Applied Chemistry},
  volume = {9},
  number = {5},
  pages = {145-153},
  doi = {10.11648/j.ajac.20210905.14},
  url = {https://doi.org/10.11648/j.ajac.20210905.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20210905.14},
  abstract = {Combretum glutinosum and Mitragyna inermis are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested in vitro on Haemonchus contortus larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on H. contortus larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, M. inermis extract had an effect only on Pseudomonas aeruginosa with a MIC of 2.5 mg/mL. C. glutinosum extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of C. glutinosum (IC50 = 8.04 µg/mL) and M. inermis (IC50 = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of C. glutinosum and could explain its activities. The results obtained from this work revealed that the tannins extracted from C. glutinosum showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from M. inermis.},
 year = {2021}
}

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TY - JOUR
T1 - Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae)
AU - Eléonore Chikani Ladekan-Yayi
AU - Placide Mahougnan Toklo
AU - Durand Dah-Nouvlessounon
AU - Mahoudo Fidèle Assogba
AU - Steven Collins Njonté Wouamba
AU - Billy Toussie Tchegnitegni
AU - Géorcelin Goué Alowanou
AU - Lamine Baba-Moussa
AU - Sylvie Hounzangbe-Adote
AU - Bruno Ndjakou Lenta
AU - Siméon Fogue Kouam
AU - Gbenou Joachim Djimon
Y1 - 2021/09/26
PY - 2021
N1 - https://doi.org/10.11648/j.ajac.20210905.14
DO - 10.11648/j.ajac.20210905.14
T2 - American Journal of Applied Chemistry
JF - American Journal of Applied Chemistry
JO - American Journal of Applied Chemistry
SP - 145
EP - 153
PB - Science Publishing Group
SN - 2330-8745
UR - https://doi.org/10.11648/j.ajac.20210905.14
AB - Combretum glutinosum and Mitragyna inermis are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested in vitro on Haemonchus contortus larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on H. contortus larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, M. inermis extract had an effect only on Pseudomonas aeruginosa with a MIC of 2.5 mg/mL. C. glutinosum extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of C. glutinosum (IC50 = 8.04 µg/mL) and M. inermis (IC50 = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of C. glutinosum and could explain its activities. The results obtained from this work revealed that the tannins extracted from C. glutinosum showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from M. inermis.
VL - 9
IS - 5
ER -

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Author Information

Eléonore Chikani Ladekan-Yayi

Department of Chemistry, University of Abomey-Calavi, Abomey-Calavi, Benin
Placide Mahougnan Toklo

Department of Chemistry, University of Abomey-Calavi, Abomey-Calavi, Benin
Durand Dah-Nouvlessounon

Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin
Mahoudo Fidèle Assogba

Department of Chemistry, University of Abomey-Calavi, Abomey-Calavi, Benin
Steven Collins Njonté Wouamba

Department of Chemistry, University of Yaounde I, Yaounde, Cameroon
Billy Toussie Tchegnitegni

Department of Chemistry, University of Dschang, Dschang, Cameroon
Géorcelin Goué Alowanou

Department of Science and Technnology of Animal Production, University of Abomey Calavi, Abomey-Calavi, Benin
Lamine Baba-Moussa

Faculty of Sciences and Technics, University of Abomey-Calavi, Abomey-Calavi, Benin
Sylvie Hounzangbe-Adote

Department of Science and Technnology of Animal Production, University of Abomey Calavi, Abomey-Calavi, Benin
Bruno Ndjakou Lenta

Department of Chemistry, University of Yaounde I, Yaounde, Cameroon
Siméon Fogue Kouam

Department of Chemistry, University of Yaounde I, Yaounde, Cameroon
Gbenou Joachim Djimon

Department of Chemistry, National University of Science, Technology, Engineering and Mathematics, Abomey, Benin

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Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, et al. (2021). Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). American Journal of Applied Chemistry, 9(5), 145-153. https://doi.org/10.11648/j.ajac.20210905.14

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ACS Style

Eléonore Chikani Ladekan-Yayi; Placide Mahougnan Toklo; Durand Dah-Nouvlessounon; Mahoudo Fidèle Assogba; Steven Collins Njonté Wouamba, et al. Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). Am. J. Appl. Chem. 2021, 9(5), 145-153. doi: 10.11648/j.ajac.20210905.14

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AMA Style

Eléonore Chikani Ladekan-Yayi, Placide Mahougnan Toklo, Durand Dah-Nouvlessounon, Mahoudo Fidèle Assogba, Steven Collins Njonté Wouamba, et al. Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae). Am J Appl Chem. 2021;9(5):145-153. doi: 10.11648/j.ajac.20210905.14

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@article{10.11648/j.ajac.20210905.14,
  author = {Eléonore Chikani Ladekan-Yayi and Placide Mahougnan Toklo and Durand Dah-Nouvlessounon and Mahoudo Fidèle Assogba and Steven Collins Njonté Wouamba and Billy Toussie Tchegnitegni and Géorcelin Goué Alowanou and Lamine Baba-Moussa and Sylvie Hounzangbe-Adote and Bruno Ndjakou Lenta and Siméon Fogue Kouam and Gbenou Joachim Djimon},
  title = {Anthelmintic and Antimicrobial Activities of Tannin Extracts of Mitragyna inermis (Willd.) Kuntze (Rubiaceae) and Combretum glutinosum Perr. ex DC (Combretaceae)},
  journal = {American Journal of Applied Chemistry},
  volume = {9},
  number = {5},
  pages = {145-153},
  doi = {10.11648/j.ajac.20210905.14},
  url = {https://doi.org/10.11648/j.ajac.20210905.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajac.20210905.14},
  abstract = {Combretum glutinosum and Mitragyna inermis are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested in vitro on Haemonchus contortus larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on H. contortus larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, M. inermis extract had an effect only on Pseudomonas aeruginosa with a MIC of 2.5 mg/mL. C. glutinosum extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of C. glutinosum (IC50 = 8.04 µg/mL) and M. inermis (IC50 = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of C. glutinosum and could explain its activities. The results obtained from this work revealed that the tannins extracted from C. glutinosum showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from M. inermis.},
 year = {2021}
}

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